Anti-Metallothionein抗体[UC1MT] (ab12228)
![Western blot - Anti-Metallothionein antibody [UC1MT] (ab12228)](https://www.abcam.cn/ps/products/12/ab12228/Images/ab12228-19787-anti-metallothionein-antibody-uc1mt-western-blot.jpg "Western blot - Anti-Metallothionein antibody [UC1MT] (ab12228)")
Key features and details
- Mouse monoclonal [UC1MT] to Metallothionein
- Suitable for: ICC/IF, WB, Flow Cyt
- Reacts with: Rabbit, Human
- Isotype: IgG1
概述
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产品名称
Anti-Metallothionein抗体[UC1MT]
参阅全部 Metallothionein 一抗 -
描述
小鼠单克隆抗体[UC1MT] to Metallothionein -
宿主
Mouse -
经测试应用
适用于: ICC/IF, WB, Flow Cytmore details -
种属反应性
与反应: Rabbit, Human -
免疫原
Full length protein corresponding to Rabbit Metallothionein. Cross-linked rabbit liver Metallothionein I and II.
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阳性对照
- HeLa cell lysate treated with 100uM CdCl2 Rehydrated rabbit liver MTI/MTII
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常规说明
This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: 2.68% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Tissue culture supernatant -
纯化说明
Purified from TCS. -
克隆
单克隆 -
克隆编号
UC1MT -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab12228于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
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| WB | (3) |
Use at an assay dependent concentration. Detects a band of approximately 6-20 kDa (predicted molecular weight: 6 kDa).
Please note: often Western blots done on cell lysates with this antibody produce many bands; we suspect that metallothionein binds to many other proteins, thus producing these results. As the predicted MW is around 6 kDa, use 12.5-20% gel and be sure the protein is not run off the gel during electrophoresis. |
| Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Detects a band of approximately 6-20 kDa (predicted molecular weight: 6 kDa). Please note: often Western blots done on cell lysates with this antibody produce many bands; we suspect that metallothionein binds to many other proteins, thus producing these results. As the predicted MW is around 6 kDa, use 12.5-20% gel and be sure the protein is not run off the gel during electrophoresis. |
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Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids. -
序列相似性
Belongs to the metallothionein superfamily. Type 1 family. -
结构域
Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines. - Information by UniProt
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数据库链接
- Entrez Gene: 4489 Human
- Entrez Gene: 4502 Human
- Omim: 156350 Human
- Omim: 156360 Human
- SwissProt: P02795 Human
- SwissProt: P04731 Human
- SwissProt: P11957 Rabbit
- SwissProt: P18055 Rabbit
see all -
别名
- CES 1 antibody
- CES1 antibody
- Metallothionein 1A antibody
see all
图片
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Western blot - Anti-Metallothionein antibody [UC1MT] (ab12228)Anti-Metallothionein antibody [UC1MT] (ab12228) + Hela cell lysate
Secondary
HRP-conjugated antibody.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 6 kDa
Exposure time: 2 minutesThis image was generated using the ascites version of the product.
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![Immunocytochemistry/ Immunofluorescence - Anti-Metallothionein antibody [UC1MT] (ab12228)](https://www.abcam.cn/ps/products/12/ab12228/Images/ab12228-19784-anti-metallothionein-antibody-uc1mt-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Metallothionein antibody [UC1MT] (ab12228)ICC/IF image of ab12228 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12228, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image was generated using the ascites version of the product.
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![Flow Cytometry - Anti-Metallothionein antibody [UC1MT] (ab12228)](https://www.abcam.cn/ps/products/12/ab12228/Images/ab12228-19782-anti-metallothionein-antibody-uc1mt-flow-cytometry.jpg)
Flow Cytometry - Anti-Metallothionein antibody [UC1MT] (ab12228)Overlay histogram showing HeLA cells stained with ab12228 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12228, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
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![Western blot - Anti-Metallothionein antibody [UC1MT] (ab12228)](https://www.abcam.cn/ps/products/12/ab12228/Images/ab12228-19785-anti-metallothionein-antibody-uc1mt-western-blot.jpg)
Western blot - Anti-Metallothionein antibody [UC1MT] (ab12228)Anti-Metallothionein antibody [UC1MT] (ab12228) at 1/1000 dilution + Rabbit liver lysates
Predicted band size: 6 kDaThis image was generated using the ascites version of the product.
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![Immunocytochemistry/ Immunofluorescence - Anti-Metallothionein antibody [UC1MT] (ab12228)](https://www.abcam.cn/ps/products/12/ab12228/Images/ab12228-19783-anti-metallothionein-antibody-uc1mt-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Metallothionein antibody [UC1MT] (ab12228)ICC/IF image of ab12228 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12228, 10µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-mouse IgG - H&L, pre-adsorbed (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image was generated using the ascites version of the product.
数据表及文件
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SDS download
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Datasheet download
文献 (57)
ab12228 被引用在 57 文献中.
- Kadota Y et al. Metallothionein Gene Deficiency Facilitates the Differentiation of C2C12 Myoblasts into Slow-Twitch Myotubes. Biol Pharm Bull 46:1240-1248 (2023). PubMed: 37661403
- Nie Y et al. Antitumor effects of cadmium against diethylnitrosamine-induced liver tumors in mice. Oncol Lett 23:33 (2022). PubMed: 34966449
- Tamano H et al. Metallothionein synthesis increased by Ninjin-yoei-to, a Kampo medicine protects neuronal death and memory loss after exposure to amyloid β1-42. J Pharm Health Care Sci 8:26 (2022). PubMed: 36316709
- Keenan J et al. Copper toxicity of inflection point in human intestinal cell line Caco-2 dissected: influence of temporal expression patterns. In Vitro Cell Dev Biol Anim 57:359-371 (2021). PubMed: 33559028
- Tamano H et al. Dehydroeffusol Pprevents Amyloid ß1-42-mediated Hippocampal Neurodegeneration via Reducing Intracellular Zn2+ Toxicity. Mol Neurobiol 58:3603-3613 (2021). PubMed: 33770339