Anti-Metabotropic Glutamate Receptor 5抗体(ab53090)
Key features and details
- Rabbit polyclonal to Metabotropic Glutamate Receptor 5
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Metabotropic Glutamate Receptor 5抗体
参阅全部 Metabotropic Glutamate Receptor 5 一抗 -
描述
兔多克隆抗体to Metabotropic Glutamate Receptor 5 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat
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免疫原
N-terminal synthetic peptide derived from human Metabotropic Glutamate Receptor 5.
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阳性对照
- WB: Mouse brain extracts. IHC-P: Human brain tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab53090 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53090于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (8) |
1/50 - 1/100.
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| WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 132 kDa.
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| 说明 |
|---|
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IHC-P
1/50 - 1/100. |
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WB
1/500 - 1/1000. Predicted molecular weight: 132 kDa. |
靶标
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功能
G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity. -
序列相似性
Belongs to the G-protein coupled receptor 3 family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2915 Human
- Entrez Gene: 108071 Mouse
- Entrez Gene: 24418 Rat
- Omim: 604102 Human
- SwissProt: P41594 Human
- SwissProt: Q3UVX5 Mouse
- SwissProt: P31424 Rat
- Unigene: 147361 Human
see all -
别名
- Glutamate receptor metabotropic 5 antibody
- GPRC1E antibody
- Grm5 antibody
see all
图片
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Western blot - Anti-Metabotropic Glutamate Receptor 5 antibody (ab53090)All lanes : Anti-Metabotropic Glutamate Receptor 5 antibody (ab53090) at 1/300 dilution
Lane 1 : Mouse brain extracts
Lane 2 : Mouse brain extracts with blocking peptide
Predicted band size: 132 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody (ab53090)Immunohistochemical analysis of paraffin embedded human brain tissue using ab53090 at 1/50 dilution, in the presence (right) and absence (left) of blocking peptide.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab53090 被引用在 16 文献中.
- Wang S et al. Electroacupuncture Regulates Pain Transition by Inhibiting the mGluR5-PKCe Signaling Pathway in the Dorsal Root Ganglia. J Pain Res 13:1471-1483 (2020). PubMed: 32606913
- Borgstedt L et al. Neurotoxicity of different amyloid beta subspecies in mice and their interaction with isoflurane anaesthesia. PLoS One 15:e0242989 (2020). PubMed: 33270674
- Bonifacino T et al. Enhanced Function and Overexpression of Metabotropic Glutamate Receptors 1 and 5 in the Spinal Cord of the SOD1G93A Mouse Model of Amyotrophic Lateral Sclerosis during Disease Progression. Int J Mol Sci 20:N/A (2019). PubMed: 31540330
- Luo WY et al. PDZ Scaffold Protein CAL Couples with Metabotropic Glutamate Receptor 5 to Protect Against Cell Apoptosis and Is a Potential Target in the Treatment of Parkinson's Disease. Neurotherapeutics 16:761-783 (2019). PubMed: 31073978
- Reichenbach B et al. Glutamate transporter Slc1a3 mediates inter-niche stem cell activation during skin growth. EMBO J 37:N/A (2018). PubMed: 29615452