重组Anti-Metabotropic Glutamate Receptor 4/MGLUR4抗体[EPR19280] (ab184302)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19280] to Metabotropic Glutamate Receptor 4/MGLUR4
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Metabotropic Glutamate Receptor 4/MGLUR4抗体[EPR19280]
参阅全部 Metabotropic Glutamate Receptor 4/MGLUR4 一抗 -
描述
兔单克隆抗体[EPR19280] to Metabotropic Glutamate Receptor 4/MGLUR4 -
宿主
Rabbit -
经测试应用
适用于: IP, WBmore details
不适用于: Flow Cyt or ICC -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human, mouse and rat cerebellum lysates; mouse cerebellum lysate (2%SDS treated, not boiled). IP: Mouse and rat cerebellum lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR19280 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab184302于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IP |
1/40.
|
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WB |
1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).
|
说明 |
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IP
1/40. |
WB
1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa). |
靶标
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功能
Receptor for glutamate. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity. -
组织特异性
Strongly expressed in the cerebellum. Expressed at low levels in hippocampus, hypothalamus and thalamus. No expression detected in liver. -
序列相似性
Belongs to the G-protein coupled receptor 3 family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2914 Human
- Entrez Gene: 268934 Mouse
- Entrez Gene: 24417 Rat
- Omim: 604100 Human
- SwissProt: Q14833 Human
- SwissProt: Q68EF4 Mouse
- SwissProt: P31423 Rat
- Unigene: 654847 Human
see all -
别名
- Glutamate receptor metabotropic 4 antibody
- GPRC1D antibody
- Grm4 antibody
see all
图片
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All lanes : Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302) at 1/1000 dilution
Lane 1 : Mouse cerebellum tissue lysate
Lane 2 : Rat cerebellum tissue lysate
Lane 3 : Mouse liver tissue lysate
Lane 4 : Rat liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 102 kDa
Observed band size: ~300 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
This protein is specially expressed at cerebellum and the expression pattern is consistent with the literature (PMID:8463825, PMID:22528491), though results may vary depending on percentage acrylamide gel used.
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Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302) at 1/1000 dilution + Mouse cerebellum lysate (2%SDS treated, not boiled) at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 102 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
This protein is specially expressed at cerebellum and the expression pattern is consistent with the literature (PMID:8463825, PMID:22528491), though results may vary depending on percentage acrylamide gel used.
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All lanes : Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302) at 1/1000 dilution
Lane 1 : Human cerebellum lysate
Lane 2 : Human fetal liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution
Predicted band size: 102 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
This protein is specially expressed at cerebellum and the expression pattern is consistent with the literature (PMID:8463825, PMID:22528491), though results may vary depending on percentage acrylamide gel used.
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Metabotropic Glutamate Receptor 4/MGLUR4 was immunoprecipitated from 1 mg of Mouse cerebellum lysate with ab184302 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184302 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: Mouse cerebellum lysate, 10μg (Input).
Lane 2: ab184302 IP in Mouse cerebellum lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab184302 in Mouse cerebellum lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
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Metabotropic Glutamate Receptor 4/MGLUR4 was immunoprecipitated from 1 mg of Rat cerebellum lysate with ab184302 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184302 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: Rat cerebellum lysate, 10μg (Input).
Lane 2: ab184302 IP in Rat cerebellum lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab184302 in Rat cerebellum lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (1)
ab184302 被引用在 1 文献中.
- Meng J et al. Nanobody-based sensors reveal a high proportion of mGlu heterodimers in the brain. Nat Chem Biol 18:894-903 (2022). PubMed: 35681029