Anti-Metabotropic Glutamate Receptor 4/MGLUR4抗体(ab53088)
Key features and details
- Rabbit polyclonal to Metabotropic Glutamate Receptor 4/MGLUR4
- Suitable for: ELISA, IHC-Fr, ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Chicken, Cat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-Metabotropic Glutamate Receptor 4/MGLUR4抗体
参阅全部 Metabotropic Glutamate Receptor 4/MGLUR4 一抗 -
描述
兔多克隆抗体to Metabotropic Glutamate Receptor 4/MGLUR4 -
宿主
Rabbit -
经测试应用
适用于: ELISA, IHC-Fr, ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Chicken, Cat, Human -
免疫原
Synthetic peptide corresponding to Human Metabotropic Glutamate Receptor 4/MGLUR4 (C terminal).
Database link: Q14833 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53088于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
|
| IHC-Fr | (5) |
Use at an assay dependent concentration.
|
| ICC/IF |
Use a concentration of 5 µg/ml.
|
|
| IHC-P | (4) |
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| WB | (3) |
1/500 - 1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).
|
| 说明 |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
ICC/IF
Use a concentration of 5 µg/ml. |
|
IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
WB
1/500 - 1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa). |
靶标
-
功能
Receptor for glutamate. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity. -
组织特异性
Strongly expressed in the cerebellum. Expressed at low levels in hippocampus, hypothalamus and thalamus. No expression detected in liver. -
序列相似性
Belongs to the G-protein coupled receptor 3 family. -
细胞定位
Cell membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 2914 Human
- Entrez Gene: 268934 Mouse
- Entrez Gene: 24417 Rat
- Omim: 604100 Human
- SwissProt: Q14833 Human
- SwissProt: Q68EF4 Mouse
- SwissProt: P31423 Rat
- Unigene: 654847 Human
see all -
别名
- Glutamate receptor metabotropic 4 antibody
- GPRC1D antibody
- Grm4 antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody (ab53088)Ab53088 staining Human cerebellum. Staining is localised to the cell membrane.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody (ab53088)All lanes : Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody (ab53088) at 1/500 dilution
Lane 1 : 293 cell extracts, treated
with Forskolin (40nM, 30mins)
Lane 2 : 293 cell extracts, treated
with Forskolin (40nM, 30mins) with peptide
Predicted band size: 102 kDa
Observed band size: 102 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody (ab53088)ICC/IF image of ab53088 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53088, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (10)
ab53088 被引用在 10 文献中.
- Piszczek L et al. Dissociation of impulsive traits by subthalamic metabotropic glutamate receptor 4. Elife 11:N/A (2022). PubMed: 34982027
- Fisher NM et al. A GRM7 mutation associated with developmental delay reduces mGlu7 expression and produces neurological phenotypes. JCI Insight 6:N/A (2021). PubMed: 33476302
- Zhang Z et al. Activation of metabotropic glutamate receptor 4 regulates proliferation and neural differentiation in neural stem/progenitor cells of the rat subventricular zone and increases phosphatase and tensin homolog protein expression. J Neurochem 156:465-480 (2021). PubMed: 32052426
- Xiao B et al. Glutamate metabotropic receptor 4 (GRM4) inhibits cell proliferation, migration and invasion in breast cancer and is regulated by miR-328-3p and miR-370-3p. BMC Cancer 19:891 (2019). PubMed: 31492116
- Wang H et al. Expression patterns of L-amino acid receptors in the murine STC-1 enteroendocrine cell line. Cell Tissue Res 378:471-483 (2019). PubMed: 31410629
- Novati A et al. Environment-dependent striatal gene expression in the BACHD rat model for Huntington disease. Sci Rep 8:5803 (2018). PubMed: 29643462
- Jenkins BG et al. Functional modulation of G-protein coupled receptors during Parkinson disease-like neurodegeneration. Neuropharmacology 108:462-73 (2016). PubMed: 26581500
- Chruscicka B et al. Tetracycline-based system for controlled inducible expression of group III metabotropic glutamate receptors. J Biomol Screen 20:350-8 (2015). PubMed: 25394730
- Betts MJ et al. Allosteric modulation of the group III mGlu receptor 4 provides functional neuroprotection in the 6-OHDA rat model of Parkinson's disease. Br J Pharmacol : (2012). PubMed: 22404342
- Wang H et al. Spinal metabotropic glutamate receptor 4 is involved in neuropathic pain. Neuroreport 22:244-8 (2011). PubMed: 21358553