AB166608

重组Anti-Metabotropic Glutamate Receptor 3/MGLUR3抗体[EPR9009(2)]

Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)]

20ul selling size
RabMAb
Recombinant
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(7 Publications)

Rabbit Recombinant Monoclonal Metabotropic Glutamate Receptor 3/MGLUR3 antibody. Suitable for WB, ICC/IF, Flow Cyt, I-ELISA and reacts with Rat, Mouse, Human, Recombinant full length protein - Human samples. Cited in 7 publications.

查看别名

GPRC1C, MGLUR3, GRM3, Metabotropic glutamate receptor 3, mGluR3

7 Images

Indirect ELISA - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (AB166608)

I-ELISA

Lab

Indirect ELISA - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (AB166608)

ELISA analysis of Human MGLUR3 recombinant protein at 500ng/ml with ab166608. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

Flow Cytometry - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (AB166608)

Flow Cyt

Lab

Flow Cytometry - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (AB166608)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neuron cells labelling Metabotropic Glutamate Receptor 3/MGLUR3 with ab166608 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Flow Cyt

Lab

Flow Cytometry - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (AB166608)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) cells labelling Metabotropic Glutamate Receptor 3/MGLUR3 with ab166608 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Unknown

Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (AB166608)

All lanes:

Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608) at 1/1000 dilution

Lane 1:

Human cerebellum lysate at 10 µg

Lane 2:

Human hypothalamus lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 99 kDa

Observed band size: 220 kDa

false

Unknown

Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (AB166608)

This blot was developed using a higher sensitivity ECL substrate.

All lanes:

Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608) at 1/1000 dilution

All lanes:

Mouse brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 99 kDa

Observed band size: 220 kDa

false

Supplier Data

Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (AB166608)

Blocking buffer and concentration : 5% NFDM/TBST. Diluting buffer and concentration : 5% NFDM/TBST. We recommend not to boil the samples after lysis to get desired WB results.

All lanes:

Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608) at 1/1000 dilution

Lane 1:

Mouse brain lysate boiled at 20 µg

Lane 2:

Mouse brain lysate unboiled at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 99 kDa

Observed band size: 220 kDa

false

Exposure time: 3s

Supplier Data

Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (AB166608)

Blocking buffer and concentration : 5% NFDM/TBST. Diluting buffer and concentration : 5% NFDM/TBST. We recommend not to boil the samples after lysis to get desired WB results.

All lanes:

Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608) at 1/1000 dilution

Lane 1:

Rat brain lysate boiled at 15 µg

Lane 2:

Rat brain lysate unboiled at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 99 kDa

Observed band size: 220 kDa

false

Exposure time: 7s

不同偶联物与剂型 (1)

Carrier free

Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR9009(2)

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human, Rat

应用

ICC/IF, WB, Flow Cyt, I-ELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity.

See full target information GRM3

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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in aging neuroscience 17:1549770 PubMed40365352

2025

Dysregulated calcium signaling in the aged macaque entorhinal cortex associated with tau hyperphosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Shveta Bathla,Dibyadeep Datta,Dinara Bolat,Elizabeth Woo,Alvaro Duque,Jon I Arellano,Amy F T Arnsten,Angus C Nairn

Cell reports 43:114278 PubMed38795347

2024

Hippocampal astrocytes induce sex-dimorphic effects on memory.

Applications

Unspecified application

Species

Unspecified reactive species

Samantha M Meadows,Fernando Palaguachi,Minwoo Wendy Jang,Avital Licht-Murava,Daniel Barnett,Till S Zimmer,Constance Zhou,Samantha R McDonough,Adam L Orr,Anna G Orr

The Journal of neuroscience : the official journal of the Society for Neuroscience 42:8897-8911 PubMed36202617

2022

Modulation of Hippocampal Network Oscillation by PICK1-Dependent Cell Surface Expression of mGlu3 Receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Pola Tuduri,Nathalie Bouquier,Benoit Girard,Enora Moutin,Maxime Thouaye,Julie Perroy,Federica Bertaso,Jeanne Ster

Frontiers in aging neuroscience 13:760270 PubMed34867287

2021

Glutamate Carboxypeptidase II in Aging Rat Prefrontal Cortex Impairs Working Memory Performance.

Applications

Unspecified application

Species

Unspecified reactive species

Dibyadeep Datta,Shannon N Leslie,Elizabeth Woo,Nishita Amancharla,Ayah Elmansy,Miguel Lepe,Adam P Mecca,Barbara S Slusher,Angus C Nairn,Amy F T Arnsten

Cell reports 31:107605 PubMed32375054

2020

Defining the Homo- and Heterodimerization Propensities of Metabotropic Glutamate Receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Joon Lee,Hermany Munguba,Vanessa A Gutzeit,Deo R Singh,Melanie Kristt,Jeremy S Dittman,Joshua Levitz

Journal of psychopharmacology (Oxford, England) 31:1519-1526 PubMed28655286

2017

A group II metabotropic glutamate receptor 3 (mGlu3, GRM3) isoform implicated in schizophrenia interacts with canonical mGlu3 and reduces ligand binding.

Applications

ICC/IF, WB

Species

Unspecified reactive species, Unspecified reactive species

Aintzane García-Bea,Isabel Bermudez,Paul J Harrison,Tracy A Lane

Schizophrenia research 177:18-27 PubMed27130562

2016

Metabotropic glutamate receptor 3 (mGlu3; mGluR3; GRM3) in schizophrenia: Antibody characterisation and a semi-quantitative western blot study.

Applications

WB, WB, ICC, ICC, IHC, IHC

Species

Human, Mouse, Human, Mouse, Mouse, Human

Aintzane García-Bea,Mary A Walker,Thomas M Hyde,Joel E Kleinman,Paul J Harrison,Tracy A Lane

View all publications

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Abcam Product Promise

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重组Anti-Metabotropic Glutamate Receptor 3/MGLUR3抗体[EPR9009(2)]