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AB281942

重组Anti-Metabotropic Glutamate Receptor 2+3/MGLUR2+MGLUR3抗体[EPR24635-155] - BSA and Azide free

Anti-Metabotropic Glutamate Receptor 2+3/MGLUR2+MGLUR3 antibody [EPR24635-155] - BSA and Azide free

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Rabbit Recombinant Monoclonal Metabotropic Glutamate Receptor 3/MGLUR3 antibody. Carrier free. Suitable for WB, IHC-P, IHC-Fr, ICC/IF and reacts with Mouse, Rat, Human samples.

查看别名

GPRC1C, MGLUR3, GRM3, Metabotropic glutamate receptor 3, mGluR3

12 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 2+3/MGLUR2+MGLUR3 antibody [EPR24635-155] - BSA and Azide free (AB281942)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 2+3/MGLUR2+MGLUR3 antibody [EPR24635-155] - BSA and Azide free (AB281942)

This data was developed using ab281922, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling Metabotropic glutamate receptor 3/MGLUR3 with ab281922 at 1/1000 dilution (0.342 ug/ml) followed by a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (ab209101). Cytoplasmic staining on human cerebrum. The section was incubated with ab281922 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 2+3/MGLUR2+MGLUR3 antibody [EPR24635-155] - BSA and Azide free (AB281942)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 2+3/MGLUR2+MGLUR3 antibody [EPR24635-155] - BSA and Azide free (AB281942)

This data was developed using ab281922, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling Metabotropic glutamate receptor 3/MGLUR3 with ab281922 at 1/1000 dilution (0.342 ug/ml) followed by a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat cerebrum. The section was incubated with ab281922 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 2+3/MGLUR2+MGLUR3 antibody [EPR24635-155] - BSA and Azide free (AB281942)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 2+3/MGLUR2+MGLUR3 antibody [EPR24635-155] - BSA and Azide free (AB281942)

This data was developed using ab281922, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling Metabotropic glutamate receptor 3/MGLUR3 with ab281922 at 1/1000 dilution (0.342 ug/ml) followed by a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse cerebrum. The section was incubated with ab281922 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 2+3/MGLUR2+MGLUR3 antibody [EPR24635-155] - BSA and Azide free (AB281942)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 2+3/MGLUR2+MGLUR3 antibody [EPR24635-155] - BSA and Azide free (AB281942)

This data was developed using ab281922, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labelling Metabotropic glutamate receptor 3/MGLUR3 with ab281922 at 1/1000 dilution (0.342 ug/ml) followed by a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse cerebellum. The section was incubated with ab281922 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 2+3/MGLUR2+MGLUR3 antibody [EPR24635-155] - BSA and Azide free (AB281942)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 2+3/MGLUR2+MGLUR3 antibody [EPR24635-155] - BSA and Azide free (AB281942)

This data was developed using ab281922, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labelling Metabotropic glutamate receptor 3/MGLUR3 with ab281922 at 1/1000 dilution (0.342 ug/ml) followed by a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (ab209101). Negative control : No staining on mouse liver. The section was incubated with ab281922 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 2+3/MGLUR2+MGLUR3 antibody [EPR24635-155] - BSA and Azide free (AB281942)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 2+3/MGLUR2+MGLUR3 antibody [EPR24635-155] - BSA and Azide free (AB281942)

This data was developed using ab281922, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver tissue labeling Metabotropic glutamate receptor 3/MGLUR3 with ab281922 at 1/100 dilution (6.84 ug/ml) followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Negative control : No staining on mouse liver (PMID : 9001707) is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 2+3/MGLUR2+MGLUR3 antibody [EPR24635-155] - BSA and Azide free (AB281942)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 2+3/MGLUR2+MGLUR3 antibody [EPR24635-155] - BSA and Azide free (AB281942)

This data was developed using ab281922, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus tissue labeling Metabotropic glutamate receptor 3/MGLUR3 with ab281922 at 1/100 dilution (3.42 ug/ml) followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Cytoplasmic staining on rat hippocampus is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 2+3/MGLUR2+MGLUR3 antibody [EPR24635-155] - BSA and Azide free (AB281942)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 2+3/MGLUR2+MGLUR3 antibody [EPR24635-155] - BSA and Azide free (AB281942)

This data was developed using ab281922, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver tissue labeling Metabotropic glutamate receptor 3/MGLUR3 with ab281922 at 1/100 dilution (6.84 ug/ml) followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Negative control : No staining on rat liver (PMID : 9001707) is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 2+3/MGLUR2+MGLUR3 antibody [EPR24635-155] - BSA and Azide free (AB281942)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 2+3/MGLUR2+MGLUR3 antibody [EPR24635-155] - BSA and Azide free (AB281942)

This data was developed using ab281922, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus tissue labeling Metabotropic glutamate receptor 3/MGLUR3 with ab281922 at 1/100 dilution (3.42 ug/ml) followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Cytoplasmic staining on mouse hippocampus is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

Western blot - Anti-Metabotropic Glutamate Receptor 2+3/MGLUR2+MGLUR3 antibody [EPR24635-155] - BSA and Azide free (AB281942)
  • WB

Lab

Western blot - Anti-Metabotropic Glutamate Receptor 2+3/MGLUR2+MGLUR3 antibody [EPR24635-155] - BSA and Azide free (AB281942)

This data was developed using 281922, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The molecular weight observed is consistent with what has been described in the literature (PMID : 27130562).

Samples are non-boiled as boiling may cause protein aggregates.

Exposure time : 10 seconds.

All lanes:

Western blot - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR24635-155] (<a href='/products/primary-antibodies/metabotropic-glutamate-receptor-2-3-mglur2-mglur3-antibody-epr24635-155-ab281922'>ab281922</a>) at 1/1000 dilution

All lanes:

Human brain tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 100 kDa,250 kDa

false

Western blot - Anti-Metabotropic Glutamate Receptor 2+3/MGLUR2+MGLUR3 antibody [EPR24635-155] - BSA and Azide free (AB281942)
  • WB

Lab

Western blot - Anti-Metabotropic Glutamate Receptor 2+3/MGLUR2+MGLUR3 antibody [EPR24635-155] - BSA and Azide free (AB281942)

This data was developed using 281922, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Negative control : liver (PMID : 9001707).

The molecular weight observed is consistent with what has been described in the literature (PMID : 27130562).

Samples are non-boiled as boiling may cause protein aggregates.

Exposure time : 3.25 seconds.

All lanes:

Western blot - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR24635-155] (<a href='/products/primary-antibodies/metabotropic-glutamate-receptor-2-3-mglur2-mglur3-antibody-epr24635-155-ab281922'>ab281922</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 40 µg

Lane 3:

Rat brain tissue lysate at 20 µg

Lane 4:

Rat liver tissue lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 100 kDa,250 kDa

false

Western blot - Anti-Metabotropic Glutamate Receptor 2+3/MGLUR2+MGLUR3 antibody [EPR24635-155] - BSA and Azide free (AB281942)
  • WB

Lab

Western blot - Anti-Metabotropic Glutamate Receptor 2+3/MGLUR2+MGLUR3 antibody [EPR24635-155] - BSA and Azide free (AB281942)

This data was developed using 281922, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The molecular weight observed is consistent with what has been described in the literature (PMID : 27130562).

Samples are non-boiled as boiling may cause protein aggregates.

Exposure time : 1 seconds.

All lanes:

Western blot - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR24635-155] (<a href='/products/primary-antibodies/metabotropic-glutamate-receptor-2-3-mglur2-mglur3-antibody-epr24635-155-ab281922'>ab281922</a>) at 1/1000 dilution

Lane 1:

HEK-293T (human embryonic kidney) transfected with an empty vector, containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

HEK-293T transfected with Metabotropic glutamate receptor 3 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 100 kDa,250 kDa

false

不同偶联物与剂型 (1)

  • Unconjugated

    Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR24635-155]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR24635-155

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse, Rat, Human

应用

ICC/IF, WB, IHC-Fr, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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产品详情

ab281942 is the carrier-free version of ab281922.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity.
See full target information GRM3

其他靶点

GRM2
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搭配使用产品

Assay Kits

AB309279

Human Metabotropic Glutamate Receptor 3/MGLUR3 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Metabotropic Glutamate Receptor 3/MGLUR3 ELISA Kit (AB309279)

  • 0
  • 0
Primary Antibodies

AB150387

Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (AB150387)

  • 5
  • 1
Primary Antibodies

AB76316

Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (AB76316)

  • 5
  • 6
Primary Antibodies

AB109450

Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479]

RabMAb|Recombinant

Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (AB109450)

  • 5
  • 2
Primary Antibodies

AB109182

Anti-NMDAR1 antibody [EPR2481(2)] - Neuronal Marker

20ul selling size|KO Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-NMDAR1 antibody [EPR2481(2)] - Neuronal Marker (AB109182)

  • 4
  • 4

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

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