AB93620

Anti-Mesothelin 抗体 [SP74]

Name: Mesothelin抗体[SP74] (ab93620)| Abcam中文官网
Brand: Abcam Limited
SKU: AB93620
Price: 1576 CNY
Availability: InStock
Rating: 5 (2 reviews)

Anti-Mesothelin antibody [SP74]

20ul selling size
RabMAb
Recombinant
了解详情

(2 Reviews)

(6 Publications)

Rabbit Recombinant Monoclonal Mesothelin antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 6 publications.

查看别名

MPF, MSLN, Mesothelin, CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [SP74] (AB93620)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [SP74] (AB93620)

Human Mesothelioma stained with Anti-Mesothelin antibody [SP74] (ab93620)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [SP74] (AB93620)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human mesothelioma tissue sections labeling Mesothelin with Purified ab93620 at 1/100 dilution (1.4 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Lab

Western blot - Anti-Mesothelin antibody [SP74] (AB93620)

Blocking/Diluting buffer and concentration : 5% NFDM /TBST

All lanes:

Western blot - Anti-Mesothelin antibody [SP74] (ab93620) at 0.322 µg/mL

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate at 20 µg

Lane 2:

BxPC-3 (Human pancreas adenocarcinoma epithelial cell) cell lysate at 20 µg

Lane 3:

PC3 (Human prostate adenocarcinoma epithelial cell) cell lysate at 20 µg

Lane 4:

A549 (Human lung carcinoma epithelial cell) cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 0.05 µg/mL

Predicted band size: 69 kDa

Observed band size: 49 kDa,69 kDa

false

不同偶联物与剂型 (7)

Carrier free

Anti-Mesothelin antibody [SP74] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Mesothelin antibody [SP74]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Mesothelin antibody [SP74]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Mesothelin antibody [SP74]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Mesothelin antibody [SP74]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Mesothelin antibody [SP74]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Mesothelin antibody [SP74]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

SP74

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.322 µg/mL", "WB-species-notes": "<p></p>" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A/G

纯化说明

Purified from TCS by protein A/G.

存储溶液

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

运输条件

Conditional Ambient

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Membrane-anchored forms may play a role in cellular adhesion.. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.

See full target information MSLN

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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:5902 PubMed40593704

2025

Generation of mature epicardium derived from human-induced pluripotent stem cells via inhibition of mTOR signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Tian,Antonio Lucena-Cacace,Kanae Tani,Amanda Putri Elvandari,Rodolfo S Allendes Osorio,Megumi Narita,Yasuko Matsumura,Ian Costa Paixao,Yutaro Miyoshi,Azusa Inagaki,Julia Junghof,Yoshinori Yoshida

Cancers 15: PubMed37345150

2023

Genomic and Transcriptomic Analyses of Malignant Pleural Mesothelioma (MPM) Samples Reveal Crucial Insights for Preclinical Testing.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander Laure,Angelica Rigutto,Michaela B Kirschner,Lennart Opitz,Linda Grob,Isabelle Opitz,Emanuela Felley-Bosco,Stefanie Hiltbrunner,Alessandra Curioni-Fontecedro

British journal of cancer 126:754-763 PubMed34876673

2021

Exploiting mesothelin in thymic carcinoma as a drug delivery target for anetumab ravtansine.

Applications

Unspecified application

Species

Unspecified reactive species

Vincent Chen,Shigeki Umemura,Yumin Han,Renuka Raman,Robin Tucker,Joeffrey Chahine,In-Kyu Kim,Christoph Schatz,Sabine Zitzmann-Kolbe,Anette Sommer,Masanori Onda,Trevor Lee,Yongfeng He,Giuseppe Giaccone

Medicine 98:e16207 PubMed31261569

2019

Mesothelin expression and its prognostic role according to microsatellite instability status in colorectal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hyunsung Kim,Yumin Chung,Seung Sam Paik,Kiseok Jang,Su-Jin Shin

The Journal of clinical investigation 127:2176-2191 PubMed28436934

2017

Biopolymers codelivering engineered T cells and STING agonists can eliminate heterogeneous tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Tyrel T Smith,Howell F Moffett,Sirkka B Stephan,Cary F Opel,Amy G Dumigan,Xiuyun Jiang,Venu G Pillarisetty,Smitha P S Pillai,K Dane Wittrup,Matthias T Stephan

International journal of clinical and experimental 6:2476-86 PubMed24228110

2013

Diagnostic and therapeutic implications of a novel immunohistochemical panel detecting duodenal mucosal invasion by pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Sabrina C Sopha,Purva Gopal,Nipun B Merchant,Frank L Revetta,David V Gold,Kay Washington,Chanjuan Shi

View all publications

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Anti-Mesothelin 抗体 [SP74]