重组Anti-Mesothelin抗体[EPR2685(2)] (ab134109)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2685(2)] to Mesothelin
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Mesothelin抗体[EPR2685(2)]
参阅全部 Mesothelin 一抗 -
描述
兔单克隆抗体[EPR2685(2)] to Mesothelin -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details
不适用于: ICC/IF -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Mesothelin aa 550 to the C-terminus. The exact sequence is proprietary.
Database link: Q13421 -
阳性对照
- WB: HeLa whole cell lysate (ab150035); Human fetal lung lysate. OVCAR-3 cell lysate IHC-P: Human mesothelioma and ovarian carcinoma tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 5.50 x 10 -12 M Learn more about KD -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2685(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134109于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 40, 69 kDa (predicted molecular weight: 69 kDa).
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IP |
1/10 - 1/100.
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 40, 69 kDa (predicted molecular weight: 69 kDa). |
IP
1/10 - 1/100. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Membrane-anchored forms may play a role in cellular adhesion.
Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro. -
组织特异性
Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level). -
疾病相关
Note=Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer. -
序列相似性
Belongs to the mesothelin family. -
翻译后修饰
Both MPF and the cleaved form of mesothelin are N-glycosylated.
Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin. -
细胞定位
Secreted and Cell membrane. Golgi apparatus. - Information by UniProt
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数据库链接
- Entrez Gene: 10232 Human
- Omim: 601051 Human
- SwissProt: Q13421 Human
- Unigene: 408488 Human
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别名
- CAK 1 antibody
- CAK1 antibody
- CAK1 antigen antibody
see all
图片
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All lanes : Anti-Mesothelin antibody [EPR2685(2)] (ab134109) at 1/1000 dilution
Lane 1 : OVCAR-3 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A549 cell lysate
Lane 4 : PC-3 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-Mesothelin antibody [EPR2685(2)] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab134109 was shown to bind specifically to Mesothelin. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR2685(2)] (ab134109)Immunohistochemical staining of paraffin embedded human skeletal muscle with purified ab134109 at a working dilution of 1/800. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Anti-Mesothelin antibody [EPR2685(2)] (ab134109) at 1/1000 dilution (purified) + HeLa whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 69 kDa
Observed band size: 69 kDa
Additional bands at: 40 kDa (possible cleavage fragment)Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
ab134109 (purified) at 1/40 immunoprecipitating mesothelin in 10 μg HeLa cell lysate (Lanes 1 and 2, observed at 40 and 69 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR2685(2)] (ab134109)
Immunohistochemical staining of paraffin embedded human mesothelioma with purified ab134109 at a working dilution of 1/800. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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All lanes : Anti-Mesothelin antibody [EPR2685(2)] (ab134109) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : Human fetal lung lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 69 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR2685(2)] (ab134109)
Immunohistochemical analysis of paraffin embedded Human mesothelioma tissue labelling Mesothelin with unpurified ab134109 at 1/1000.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR2685(2)] (ab134109)
Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue labelling Mesothelin with unpurified ab134109 at 1/1000.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR2685(2)] (ab134109)
Immunohistochemical analysis of paraffin embedded normal Human pancreas tissue using unpurified ab134109 showing -ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR2685(2)] (ab134109)
Immunohistochemical analysis of paraffin embedded Human Pancreatic carcinoma tissue using unpurified ab134109 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR2685(2)] (ab134109)
Immunohistochemical analysis of paraffin embedded Human Greator omentum tissue using unpurified ab134109 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab134109 被引用在 1 文献中.
- Hassan R et al. Clinical Response of Live-Attenuated, Listeria monocytogenes Expressing Mesothelin (CRS-207) with Chemotherapy in Patients with Malignant Pleural Mesothelioma. Clin Cancer Res 25:5787-5798 (2019). PubMed: 31263030