AB242528

重组Anti-Mesothelin抗体[EPR19025-42] - BSA and Azide free (Capture)

Anti-Mesothelin antibody [EPR19025-42] - BSA and Azide free (Capture)

RabMAb
Recombinant
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Anti-Mesothelin antibody [EPR19025-42] - BSA and Azide free (Capture) (ab242528) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting Mesothelin in Western Blot, ELISA. Suitable for Human.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency

查看别名

MPF, MSLN, Mesothelin, CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor

2 Images

Lab

Western blot - Anti-Mesothelin antibody [EPR19025-42] - BSA and Azide free (Capture) (AB242528)

This image was produced using ab196235, the same clone but in a different formulation.

False colour image of Western blot : Anti-Mesothelin antibody [EPR19025-42] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab196235 was shown to bind specifically to Mesothelin. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-Mesothelin antibody [EPR19025-42] (<a href='/products/primary-antibodies/mesothelin-antibody-epr19025-42-ab196235'>ab196235</a>) at 1/1000 dilution

Lane 1:

OVCAR-3 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

A549 cell lysate at 20 µg

Lane 4:

PC-3 cell lysate at 20 µg

Predicted band size: 69 kDa

Observed band size: 45 kDa

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Sandwich ELISA - Anti-Mesothelin antibody [EPR19025-42] - BSA and Azide free (Capture) (AB242528)

sELISA

Supplier Data

Sandwich ELISA - Anti-Mesothelin antibody [EPR19025-42] - BSA and Azide free (Capture) (AB242528)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab216168).

不同偶联物与剂型 (5)

Unconjugated

Anti-Mesothelin antibody [EPR19025-42]
Carrier free

Anti-Mesothelin antibody [EPR19025-42] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Mesothelin antibody [EPR19025-42]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Mesothelin antibody [EPR19025-42]
578 PE

PE Anti-Mesothelin antibody [EPR19025-42]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR19025-42

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

sELISA, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

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产品详情

ab242528 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Mesothelin. The recommended pair for sandwich ELISA is:
Capture: ab242528, Human Mesothelin Capture Antibody (unconjugated)
Detector: ab242775, Human Mesothelin Detector Antibody (unconjugated)
The reference range value is 15.62 - 1000 pg/ml.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Constituents: PBS

运输条件

Blue Ice

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Membrane-anchored forms may play a role in cellular adhesion.. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.

See full target information MSLN

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Abcam Product Promise

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详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-Mesothelin抗体[EPR19025-42] - BSA and Azide free (Capture)