重组Anti-MERTK抗体[EPR23248-259] - BSA and Azide free (ab272177)
![Western blot - Anti-MERTK antibody [EPR23248-259] - BSA and Azide free (ab272177)](https://www.abcam.cn/ps/products/272/ab272177/Images/ab272177-392896-anti-mertk-antibody-epr23248-259-western-blot-ovary-testis-human.jpg "Western blot - Anti-MERTK antibody [EPR23248-259] - BSA and Azide free (ab272177)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23248-259] to MERTK - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-MERTK抗体[EPR23248-259] - BSA and Azide free
参阅全部 MERTK 一抗 -
描述
兔单克隆抗体[EPR23248-259] to MERTK - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-Fr,IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HEK-293, HepG2, U-937, Human spleen, Human ovary, Human testis, Human liver, Mouse spleen, Mouse kidney and Rat spleen, Rat kidney lysates.
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常规说明
ab272177 is the carrier-free version of ab270448.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23248-259 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272177于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 110 kDa.
|
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 110 kDa. |
靶标
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功能
In case of filovirus infection, seems to function as a cell entry factor. -
组织特异性
Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines. -
疾病相关
Defects in MERTK are the cause of retinitis pigmentosa type 38 (RP38) [MIM:613862]. RP38 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 10461 Human
- Entrez Gene: 17289 Mouse
- Entrez Gene: 65037 Rat
- Omim: 604705 Human
- SwissProt: Q12866 Human
- SwissProt: Q60805 Mouse
- SwissProt: P57097 Rat
- Unigene: 306178 Human
see all -
别名
- c MER antibody
- c mer proto oncogene tyrosine kinase antibody
- c-mer antibody
see all
图片
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Western blot - Anti-MERTK antibody [EPR23248-259] - BSA and Azide free (ab272177)All lanes : Anti-MERTK antibody [EPR23248-259] (ab270448) at 1/1000 dilution
Lane 1 : Human ovary tissue lysate
Lane 2 : Human testis tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 110 kDa
Observed band size: 150, 210 kDa why is the actual band size different from the predicted?Exposure time:
Lane 1: 3 minutes
Lane 2: 70 secondsBlocking/Diluting buffer: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270448).
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![Anti-MERTK antibody [EPR23248-259] - BSA and Azide free (ab272177)](https://www.abcam.cn/ps/products/272/ab272177/Images/ab272177-4-benefits-of-recombinant-antibodies.png)
Anti-MERTK antibody [EPR23248-259] - BSA and Azide free (ab272177)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab272177 尚未被引用在任何文献中。
