Anti-MERTK抗体[A311F9G3] (ab52591)
![Flow Cytometry - Anti-MERTK antibody [A311F9G3] (ab52591)](https://www.abcam.cn/ps/products/52/ab52591/Images/ab52591-212986-anti-mertk-antibody-a311f9g3-flow-cytometry.jpg "Flow Cytometry - Anti-MERTK antibody [A311F9G3] (ab52591)")
Key features and details
- Mouse monoclonal [A311F9G3] to MERTK
- Suitable for: Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-MERTK抗体[A311F9G3]
参阅全部 MERTK 一抗 -
描述
小鼠单克隆抗体[A311F9G3] to MERTK -
宿主
Mouse -
经测试应用
适用于: Flow Cyt, WBmore details -
种属反应性
与反应: Human -
免疫原
Human MERTK extracellular domain.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
A311F9G3 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52591于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| WB |
1/1000. Predicted molecular weight: 110 kDa.
|
| 说明 |
|---|
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Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000. Predicted molecular weight: 110 kDa. |
靶标
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功能
In case of filovirus infection, seems to function as a cell entry factor. -
组织特异性
Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines. -
疾病相关
Defects in MERTK are the cause of retinitis pigmentosa type 38 (RP38) [MIM:613862]. RP38 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 10461 Human
- Omim: 604705 Human
- SwissProt: Q12866 Human
- Unigene: 306178 Human
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别名
- c MER antibody
- c mer proto oncogene tyrosine kinase antibody
- c-mer antibody
see all
图片
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Flow Cytometry - Anti-MERTK antibody [A311F9G3] (ab52591)
Overlay histogram showing HEK293 cells stained with ab52591 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52591, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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![Western blot - Anti-MERTK antibody [A311F9G3] (ab52591)](https://www.abcam.cn/ps/products/52/ab52591/Images/ab52591-172408-anti-mertk-antibody-a311f9g3-western-blot.jpg)
Western blot - Anti-MERTK antibody [A311F9G3] (ab52591)ab52591 used at 1/1000
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab52591 被引用在 4 文献中.
- Murase H et al. Progranulin increases phagocytosis by retinal pigment epithelial cells in culture. J Neurosci Res 95:2500-2510 (2017). PubMed: 28509387
- Zahuczky G et al. Differentiation and glucocorticoid regulated apopto-phagocytic gene expression patterns in human macrophages. Role of mertk in enhanced phagocytosis. PLoS One 6:e21349 (2011). WB ; Human . PubMed: 21731712
- Graham DK et al. Ectopic expression of the proto-oncogene Mer in pediatric T-cell acute lymphoblastic leukemia. Clin Cancer Res 12:2662-9 (2006). PubMed: 16675557
- Graham DK et al. Cloning and mRNA expression analysis of a novel human protooncogene, c-mer. Cell Growth Differ 5:647-57 (1994). PubMed: 8086340