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AB316333

重组Anti-MERS-Cov Nucleocapsid蛋白抗体[abd211] - BSA and Azide free

Anti-MERS-Cov Nucleocapsid protein antibody [abd211] - BSA and Azide free

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Human Recombinant Monoclonal NCAP antibody. Carrier free. Suitable for WB, ICC/IF, I-ELISA and reacts with Recombinant fragment - MERS-CoV, Transfected cell lysate - MERS-CoV, Transfected cell line - MERS-CoV samples.
4 Images
Immunocytochemistry/ Immunofluorescence - Anti-MERS-Cov Nucleocapsid protein antibody [abd211] - BSA and Azide free (AB316333)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MERS-Cov Nucleocapsid protein antibody [abd211] - BSA and Azide free (AB316333)

This data was developed using ab316332, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) transfected with a MERS-CoV Nucleocapsid protein expression vector containing a myc tag. cells labelling MERS-Cov Nucleocapsid protein with ab316332 at 1/200 (5.08 ug/ml) dilution, followed by Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) antibody at 1/100 (5ug/ml) dilution (Green).

Confocal image showing cytoplasmic staining in 293T cells transfected with MERS-CoV Nucleocapsid expression vector containing a Myc-his-tag® (shown in green). The counterstain was observed in red. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223895 Anti-Myc tag Mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain at 1/100 (5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) at 1/100 (5ug/ml) dilution.

Indirect ELISA - Anti-MERS-Cov Nucleocapsid protein antibody [abd211] - BSA and Azide free (AB316333)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-MERS-Cov Nucleocapsid protein antibody [abd211] - BSA and Azide free (AB316333)

This data was developed using ab316332, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab316332 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Human IgG (H+L) at 1 : 1000 dilution.

Antigen : MERS-CoV Nucleocapsid protein,HCoV-NL63 Nucleocapsid protein,SARS-CoV Nucleocapsid protein,HCoV-OC43 Nucleocapsid protein,HCoV-HKU1 Nucleocapsid protein,HCoV-229E Nucleocapsid protein.

Antigen concentration : 1000 ng/ml

Western blot - Anti-MERS-Cov Nucleocapsid protein antibody [abd211] - BSA and Azide free (AB316333)
  • WB

Supplier Data

Western blot - Anti-MERS-Cov Nucleocapsid protein antibody [abd211] - BSA and Azide free (AB316333)

This data was developed using ab316332, the same antibody clone in a different buffer formulation.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-MERS-Cov Nucleocapsid protein antibody [abd211] (<a href='/products/primary-antibodies/mers-cov-nucleocapsid-protein-antibody-abd211-ab316332'>ab316332</a>) at 1/1000 dilution

All lanes:

His-tagged MERS-CoV Nucleocapsid recombinant protein at 10 ng with 5% NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Human IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-human-igg-h-l-hrp-ab6858'>ab6858</a>) at 1/5000 dilution

Observed band size: 46 kDa

false

Exposure time: 180s

Western blot - Anti-MERS-Cov Nucleocapsid protein antibody [abd211] - BSA and Azide free (AB316333)
  • WB

Supplier Data

Western blot - Anti-MERS-Cov Nucleocapsid protein antibody [abd211] - BSA and Azide free (AB316333)

This data was developed using ab316332, the same antibody clone in a different buffer formulation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-MERS-Cov Nucleocapsid protein antibody [abd211] (<a href='/products/primary-antibodies/mers-cov-nucleocapsid-protein-antibody-abd211-ab316332'>ab316332</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector (vector control) conteining a myc-His-tag®, whole cell lysate at 20 µg with 5% NFDM/TBST

Lane 2:

293T transfected with a MERS-CoV Nucleocapsid Protein expression vector conteining a myc-His-tag®, whole cell lysate at 20 µg with 5% NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Human IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-human-igg-h-l-hrp-ab6858'>ab6858</a>) at 1/5000 dilution

Observed band size: 46 kDa,36 kDa

false

Exposure time: 1s

关键信息

宿主种属

Human

克隆

Monoclonal

克隆号

abd211

亚型

IgG1

不含载体蛋白

Yes

反应种属

MERS-CoV

应用

WB, ICC/IF, I-ELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

This antibody does not cross-react with recombinant Human coronavirus NL63 nucleocapsid, SARS-CoV nucleocapsid, Human coronavirus OC43 nucleocapsid, Human coronavirus HKU1-Cov nucleocapsid and Human coronavirus 229E nucleocapsid.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "MERS-CoV": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Recombinant fragment - MERS-CoV": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>" }, "Transfected cell line - MERS-CoV": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Transfected cell lysate - MERS-CoV": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" } } }
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产品详情

ab316333 is the carrirer-free version of ab316332.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The MERS-CoV Nucleocapsid protein also called the N protein plays an important role in the assembly process of the Middle East Respiratory Syndrome Coronavirus (MERS-CoV). The N protein is a structural component that packages the viral RNA genome into a helical ribonucleocapsid which assists in protecting the RNA. This protein which has a mass of approximately 48 kDa is expressed during the viral infection in the host cells particularly in the cytoplasm where it facilitates the encapsulation of the viral genome.
Biological function summary

The MERS-CoV Nucleocapsid protein interacts closely with other viral proteins including the membrane (M) protein to ensure successful viral replication and assembly. This protein does not work alone; it is part of the larger ribonucleoprotein complex which plays an essential role in stabilizing the viral RNA. Its interaction with the M protein helps in localizing the N protein to specific sites in the host cell preparing the components necessary for virion assembly and release.

Pathways

The MERS-CoV Nucleocapsid protein engages in processes important for viral pathogenesis and host cell interaction. The protein is part of the coronavirus replication and transcription complex pathway. Within this matrix it associates with non-structural proteins like the RNA-dependent RNA polymerase to enhance RNA synthesis. Additionally it partakes in modulation of host processes to evade the immune response a pivotal aspect of how coronaviruses maintain infection and propagate within the host.

The MERS-CoV Nucleocapsid protein is intimately associated with Middle East Respiratory Syndrome (MERS) a severe respiratory illness. This protein aids the virus in evading the host immune response potentially contributing to disease severity. N protein interactions with host cellular machinery combined with the immune evasion tactics offer insights into viral pathogenicity and might reveal potential therapeutic targets. Understanding these interactions can shed light on how interventions might disrupt the viral life cycle and ameliorate disease impact.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

See full target information MERS-CoV Nucleocapsid
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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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