重组Anti-MELK抗体[EPR3981] - BSA and Azide free (ab172889)
![Western blot - Anti-MELK antibody [EPR3981] - BSA and Azide free (ab172889)](https://www.abcam.cn/ps/products/172/ab172889/Images/ab172889-448182-anti-melk-antibody-epr3981-bsa-and-azide-free-western-blot-hct116-hct116-melk-knockout.jpg "Western blot - Anti-MELK antibody [EPR3981] - BSA and Azide free (ab172889)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3981] to MELK - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-MELK抗体[EPR3981] - BSA and Azide free
参阅全部 MELK 一抗 -
描述
兔单克隆抗体[EPR3981] to MELK - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: HCT116, HAP1, HeLa, K562, Jurkat and HEK-293T cell lysates.
-
常规说明
ab172889 is the carrier-free version of ab108529.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3981 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Conjugation kits
-
Isotype control
-
KO cell lines
-
KO cell lysates
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab172889于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 75 kDa.
|
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 75 kDa. |
靶标
-
功能
Phosphorylates ZNF622 and may contribute to its redirection to the nucleus. May be involved in the inhibition of spliceosome assembly during mitosis. -
组织特异性
Expressed in placenta, kidney, thymus, testis, ovary and intestine. -
序列相似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 KA1 (kinase-associated) domain.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated. Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8. -
细胞定位
Cytoplasm. - Information by UniProt
-
数据库链接
- Entrez Gene: 9833 Human
- Omim: 607025 Human
- SwissProt: Q14680 Human
- Unigene: 184339 Human
-
别名
- AI327312 antibody
- hMELK antibody
- HPK 38 antibody
see all
图片
-
Western blot - Anti-MELK antibody [EPR3981] - BSA and Azide free (ab172889)All lanes : Anti-MELK antibody [EPR3981] (ab108529) at 1/1000 dilution
Lane 1 : HCT116 cell lysate
Lane 2 : MELK knockout HCT116 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 75 kDa
Observed band size: 75 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab108529).
Lanes 1 - 2: Merged signal (red and green). Green - ab108529 observed at 75 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab108529 was shown to react with MELK in wild-type HCT116 cells in western blot with loss of signal observed in MELK knockout cell line ab266896 (MELK knockout cell lysate ab257537). Wild-type and MELK knockout HCT116 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab108529 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
![Western blot - Anti-MELK antibody [EPR3981] - BSA and Azide free (ab172889)](https://www.abcam.cn/ps/products/172/ab172889/Images/ab172889-403267-anti-melk-antibody-epr3981-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-MELK antibody [EPR3981] - BSA and Azide free (ab172889)This data was developed using ab108529, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: MELK knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: HCT116 cell lysate (20 µg)
Lanes 1 - 4 Merged signal (red and green). Green - ab108529 observed at 75 kDa. Red - loading control, ab8226, observed at 42 kDa.
ab108529 was shown to recognize MELK when MELK knockout samples were used, along with additional cross-reactive bands. Wild-type and MELK knockout samples were subjected to SDS-PAGE. ab108529 and ab8226 (loading control to beta Actin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
-
![Western blot - Anti-MELK antibody [EPR3981] - BSA and Azide free (ab172889)](https://www.abcam.cn/ps/products/172/ab172889/Images/ab172889-398186-anti-melk-antibody-epr3981-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-MELK antibody [EPR3981] - BSA and Azide free (ab172889)All lanes : Anti-MELK antibody [EPR3981] (ab108529) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 75 kDaThis data was developed using ab108529, the same antibody clone in a different buffer formulation.
-
![Anti-MELK antibody [EPR3981] - BSA and Azide free (ab172889)](https://www.abcam.cn/ps/products/172/ab172889/Images/ab172889-3-benefits-of-recombinant-antibodies.png)
Anti-MELK antibody [EPR3981] - BSA and Azide free (ab172889)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
Datasheet download
Certificate of Compliance
文献 (0)
ab172889 尚未被引用在任何文献中。
