AB108529

重组Anti-MELK抗体[EPR3981]

Anti-MELK antibody [EPR3981]

RabMAb
Recombinant
KO Validated
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(16 Publications)

Rabbit Recombinant Monoclonal MELK antibody. Suitable for WB and reacts with Human samples. Cited in 16 publications.

查看别名

KIAA0175, MELK, Maternal embryonic leucine zipper kinase, hMELK, Protein kinase Eg3, Protein kinase PK38, Tyrosine-protein kinase MELK, pEg3 kinase, hPK38

3 Images

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Western blot - Anti-MELK antibody [EPR3981] (AB108529)

All lanes:

Western blot - Anti-MELK antibody [EPR3981] (ab108529) at 1/1000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

HRP-labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 74 kDa

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Lab

Western blot - Anti-MELK antibody [EPR3981] (AB108529)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : MELK knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : HCT116 cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab108529 observed at 75 kDa. Red - loading control, ab8226, observed at 42 kDa.

ab108529 was shown to recognize MELK when MELK knockout samples were used, along with additional cross-reactive bands. Wild-type and MELK knockout samples were subjected to SDS-PAGE. ab108529 and ab8226 (loading control to beta Actin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-MELK antibody [EPR3981] (ab108529)

Predicted band size: 74 kDa

false

Lab

Western blot - Anti-MELK antibody [EPR3981] (AB108529)

Lanes 1 - 2 : Merged signal (red and green). Green - ab108529 observed at 75 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab108529 was shown to react with MELK in wild-type HCT116 cells in western blot with loss of signal observed in MELK knockout cell line ab266896 (MELK knockout cell lysate ab257537). Wild-type and MELK knockout HCT116 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab108529 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-MELK antibody [EPR3981] (ab108529) at 1/1000 dilution

Lane 1:

HCT116 cell lysate at 20 µg

Lane 2:

MELK knockout HCT116 cell lysate at 20 µg

Lane 2:

Western blot - Human MELK knockout HCT116 cell line (<a href='/products/cell-lines/human-melk-knockout-hct116-cell-line-ab266896'>ab266896</a>)

Predicted band size: 74 kDa

Observed band size: 75 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-MELK antibody [EPR3981] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR3981

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Maternal embryonic leucine zipper kinase commonly referred to as MELK is a serine/threonine protein kinase with a molecular mass of about 74 kDa. MELK is broadly expressed in both human and animal tissues with significant presence in embryonic and adult stem cells. Researchers often recognize it as MELK or MELK-0A21 in studies. The protein is characterized by its regulation of cell division and apoptosis making it an important player in cellular proliferation.

Biological function summary

MELK regulates signals that control the cell cycle apoptosis and embryonic development. MELK works as a component of complexes involved in maintaining cellular homeostasis. The protruding roles involve influencing cellular architecture and governance of stem cell maintenance. Studies in animal models show MELK involvement in maintaining pluripotency and regulation during the development phase.

Pathways

MELK participates in key processes such as the cell cycle and apoptosis pathways. Its diverse functionality connects MELK to proteins like Bcl-2-associated death promoter (BAD) and p53 which further influence main cellular events. These pathways and interactions make MELK a target of interest in the study of cellular growth and programmed cell death.

Researchers link MELK to cancer and neurodegenerative diseases. The overexpression of MELK is frequently observed in various cancer types making it relevant to oncogenesis. Studies examine its relation to proteins like cyclin-dependent kinases (CDKs) and survivin in cancer progression. Additionally MELK-0A21 variants may offer insights in disorders like Glioblastoma where abnormal MELK activity aligns with disease aggression.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.

See full target information MELK

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文献 (16)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in microbiology 16:1600935 PubMed40539108

2025

Screening kinase inhibitors identifies MELK as a prime target against influenza virus infections through inhibition of viral mRNA splicing.

Applications

Unspecified application

Species

Unspecified reactive species

Xuanye Yang,Xili Feng,Qianyun Liu,Lele An,Zhongren Ma,Xiaoxia Ma

Experimental and therapeutic medicine 26:480 PubMed37745040

2023

Integrated bioinformatics analysis and experimental validation reveals hub genes of rheumatoid arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Luo,Yumei Zhong,Yanding Guo,Jingwei Nie,Yimei Xu,Haiyan Zhou

G3 (Bethesda, Md.) 11: PubMed34550356

2021

A synthetic lethal screen identifies HDAC4 as a potential target in MELK overexpressing cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Zhou,Siqi Zheng,Fernando R Rosas Bringas,Bjorn Bakker,Judith E Simon,Petra L Bakker,Hinke G Kazemier,Michael Schubert,Maurits Roorda,Marcel A T M van Vugt,Michael Chang,Floris Foijer

Stem cell research & therapy 11:455 PubMed33109266

2020

microRNA-375 released from extracellular vesicles of bone marrow mesenchymal stem cells exerts anti-oncogenic effects against cervical cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Ding,Jinhua Liu,Xiaofei Zhang

Oncology reports 44:1037-1048 PubMed32705239

2020

Maternal embryonic leucine zipper kinase serves as a poor prognosis marker and therapeutic target in osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Salim F A Jeddo,Xianfu Wei,Ka Li,Xin Li,Qiang Yang,Samina Dongol,Jianmin Li

Cell transplantation 28:37S-50S PubMed31813279

2019

MELK is Upregulated in Advanced Clear Cell Renal Cell Carcinoma and Promotes Disease Progression by Phosphorylating PRAS40.

Applications

Unspecified application

Species

Unspecified reactive species

Han Zhang,Pengtao Wei,Wenwei Lv,Xingtao Han,Jinhui Yang,Shuaifeng Qin,Yue Zhang

Cell death & disease 10:568 PubMed31358735

2019

Long noncoding RNA LINC02418 regulates MELK expression by acting as a ceRNA and may serve as a diagnostic marker for colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yinghui Zhao,Tiantian Du,Lutao Du,Peilong Li,Juan Li,Weili Duan,Yunshan Wang,Chuanxin Wang

iScience 9:149-160 PubMed30391850

2018

A Conditional Dependency on MELK for the Proliferation of Triple-Negative Breast Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yubao Wang,Ben B Li,Jing Li,Thomas M Roberts,Jean J Zhao

eLife 7: PubMed29417930

2018

MELK expression correlates with tumor mitotic activity but is not required for cancer growth.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher J Giuliano,Ann Lin,Joan C Smith,Ann C Palladino,Jason M Sheltzer

Cancer cell international 17:102 PubMed29151817

2017

MicroRNA-214-3p inhibits proliferation and cell cycle progression by targeting MELK in hepatocellular carcinoma and correlates cancer prognosis.

Applications

IHC-P, WB

Species

Human, Human

Yue Li,You Li,Yao Chen,Qian Xie,Ningning Dong,Yanjun Gao,Huan Deng,Chunhua Lu,Suihai Wang

View all publications

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重组Anti-MELK抗体[EPR3981]