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AB787

Anti-Melanoma gp100 抗体 [HMB45]

Anti-Melanoma gp100 antibody [HMB45]

3

(3 Reviews)

|

(43 Publications)

Mouse Monoclonal Melanoma gp100 antibody. Suitable for Flow Cyt, IHC-P, ICC/IF and reacts with Human samples. Cited in 43 publications. Immunogen corresponding to Cell preparation containing PMEL protein.

查看别名

D12S53E, PMEL17, SILV, PMEL, Melanocyte protein PMEL, ME20-M, Melanocyte protein Pmel 17, Melanocytes lineage-specific antigen GP100, Melanoma-associated ME20 antigen, P1, P100, Premelanosome protein, Silver locus protein homolog, ME20M

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [HMB45] (AB787)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [HMB45] (AB787)

Formalin-fixed, paraffin-embedded human testis tissue stained with ab787 at 1 μg/ml in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [HMB45] (AB787)
  • IHC-P

AbReview17800****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [HMB45] (AB787)

ab787 staining Melanoma in the human Melanoma cell line WM3248 xenopraph by IHC-P (formaldehyde-fixed paraffin-embedded sections). Tissue samples were fixed with formaldehyde; permeabilized with 0.1% Triton X-100 and blocked with 100% Dakocytomation X0909 for 1 hour at room temperature; antigen retrival was by heat mediation in Citric buffer (pH6). The sample was incubated with primary antibody (1/25) at 4°C for 18 hours. An HRP-conjugated Goat polyclonal to mouse IgG (1/100) was used as secondary antibody.

This image is courtesy of an Abreview submitted by Dr. Hongwei Shao

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [HMB45] (AB787)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [HMB45] (AB787)

Formalin-fixed, paraffin-embedded human melanoma tissue stained with ab787 at 1 μg/ml in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [HMB45] (AB787)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [HMB45] (AB787)

ab787 - immunohistochemistry

Formalin fixed paraffin embedded human melanoma stained with ab787, using ABC and AEC chromogen.

Flow Cytometry - Anti-Melanoma gp100 antibody [HMB45] (AB787)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-Melanoma gp100 antibody [HMB45] (AB787)

Overlay histogram showing Malme-3 cells stained with ab787 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab787, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Malme-3 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

HMB45

亚型

IgG1

轻链类型

kappa

不含载体蛋白

No

反应种属

Human

应用

ICC/IF, IHC-P, Flow Cyt

applications

免疫原

Cell preparation containing PMEL protein. The exact immunogen used to generate this antibody is proprietary information.

P40967

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/10", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p>Incubate for 30 minutes at room temperature. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/10", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p>Incubate for 30 minutes at room temperature. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes</p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Dog": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/10", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p>Incubate for 30 minutes at room temperature. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes</p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

性能和储存信息

形式
Liquid
纯度
Tissue culture supernatant
存储溶液
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: Tissue culture supernatant, 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
储存信息
Avoid freeze / thaw cycle

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

Forms physiological amyloids that play a central role in melanosome morphogenesis and pigmentation. The maturation of unpigmented premelanosomes from stage I to II is marked by assembly of processed amyloidogenic fragments into parallel fibrillar sheets, which elongate the vesicle into a striated ellipsoidal shape. In pigmented stage III and IV melanosomes, the amyloid matrix serves as a platform where eumelanin precursors accumulate at high local concentrations for pigment formation. May prevent pigmentation-associated toxicity by sequestering toxic reaction intermediates of eumelanin biosynthesis pathway.. Represents a potent melanoma-specific antigen. Among melanoma non-mutated self-peptides, G9-154 (KTWGQYWQV), G9-209 (ITDQVPFSV) and G9-280 (YLEPGPVTA), appear to act as immunodominant common epitopes that stimulate anti-tumor immune response mediated by HLA-A-restricted cytotoxic T cells.
See full target information PMEL

文献 (43)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 35:102357 PubMed39618825

2024

Local CpG- siRNA treatment improves antitumor effects of immune checkpoint inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyan Zhang,Rui Huang,Lyuzhi Ren,Antons Martincuks,JiEun Song,Marcin Kortylewski,Piotr Swiderski,Stephen J Forman,Hua Yu

Investigative ophthalmology & visual science 65:7 PubMed38833258

2024

Radiation-Induced DNA Damage in Uveal Melanoma Is Influenced by Dose Delivery and Chromosome 3 Status.

Applications

Unspecified application

Species

Unspecified reactive species

Aysegül Tura,Yingda Zhu,Siranush Vardanyan,Michelle Prasuhn,Vinodh Kakkassery,Julia Lüke,Hartmut Merz,Frank Paulsen,Dirk Rades,Florian Cremers,Karl-Ulrich Bartz-Schmidt,Salvatore Grisanti

Proceedings of the National Academy of Sciences of the United States of America 121:e2321323121 PubMed38607931

2024

Extracellular vesicles released by keratinocytes regulate melanosome maturation, melanocyte dendricity, and pigment transfer.

Applications

Unspecified application

Species

Unspecified reactive species

Marie-Thérèse Prospéri,Cécile Giordano,Mireia Gomez-Duro,Ilse Hurbain,Anne-Sophie Macé,Graça Raposo,Gisela D'Angelo

Nature communications 14:7759 PubMed38030596

2023

HDAC8-mediated inhibition of EP300 drives a transcriptional state that increases melanoma brain metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Michael F Emmons,Richard L Bennett,Alberto Riva,Kanchan Gupta,Larissa Anastasio Da Costa Carvalho,Chao Zhang,Robert Macaulay,Daphne Dupéré-Richér,Bin Fang,Edward Seto,John M Koomen,Jiannong Li,Y Ann Chen,Peter A Forsyth,Jonathan D Licht,Keiran S M Smalley

Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) 29:e13345 PubMed37231929

2023

The effects of miRNA-27a-3p on human epidermal melanocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Li,Suqin Wang,Jing Ning,Xiaoqian Mao,Kang Ge,Ruzhi Zhang

International journal of molecular sciences 24: PubMed37175529

2023

Efficient Isolation and Expansion of Limbal Melanocytes for Tissue Engineering.

Applications

Unspecified application

Species

Unspecified reactive species

Naresh Polisetti,Thomas Reinhard,Günther Schlunck

The Journal of investigative dermatology 143:317-327.e6 PubMed36063887

2022

S-Palmitoylation of Tyrosinase at Cysteine Regulates Melanogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yoko Niki,Naoko Adachi,Masaki Fukata,Yuko Fukata,Shinichiro Oku,Chieko Makino-Okamura,Seiji Takeuchi,Kazumasa Wakamatsu,Shosuke Ito,Lieve Declercq,Daniel B Yarosh,Tomas Mammone,Chikako Nishigori,Naoaki Saito,Takehiko Ueyama

Frontiers in oncology 11:644592 PubMed34178631

2021

Normalization of Enzyme Expression and Activity Regulating Vitamin A Metabolism Increases RAR-Beta Expression and Reduces Cellular Migration and Proliferation in Diseases Caused by Tuberous Sclerosis Gene Mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Elhusseiny Mohamed Mahmoud Abdelwahab,Judit Bovari-Biri,Gabor Smuk,Tunde Harko,Janos Fillinger,Judit Moldvay,Vera P Krymskaya,Judit E Pongracz

Cell and tissue banking 22:67-75 PubMed32978700

2020

Dedifferentiation of human epidermal melanocytes in vitro by long-term trypsinization.

Applications

Unspecified application

Species

Unspecified reactive species

Ren-He Chen,Li Xiao,Ru-Zhi Zhang,Sheng-Yi Wang,Yue Li

Nature communications 11:2988 PubMed32532976

2020

Coupling of melanocyte signaling and mechanics by caveolae is required for human skin pigmentation.

Applications

Unspecified application

Species

Unspecified reactive species

Lia Domingues,Ilse Hurbain,Floriane Gilles-Marsens,Julia Sirés-Campos,Nathalie André,Melissa Dewulf,Maryse Romao,Christine Viaris de Lesegno,Anne-Sophie Macé,Cédric Blouin,Christelle Guéré,Katell Vié,Graça Raposo,Christophe Lamaze,Cédric Delevoye
View all publications

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