Anti-Melanoma抗体[PNL2] (ab12502)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [PNL2] (ab12502)](https://www.abcam.cn/ps/products/12/ab12502/Images/ab12502-20060-anti-melanoma-antibody-pnl2-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [PNL2] (ab12502)")
Key features and details
- Mouse monoclonal [PNL2] to Melanoma
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Melanoma抗体[PNL2]
参阅全部 Melanoma 一抗 -
描述
小鼠单克隆抗体[PNL2] to Melanoma -
宿主
Mouse -
特异性
This antibody labels melanocytes in the cytoplasm and plasma membrane and is useful tool for the identification of melanomas and clear cell sarcomas. It seems to be a melanocyte antibody of autoimmune origin as it does not react with the non-melanocytic peptide antigen used for immunization. -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
The details of the immunogen for this antibody are not available.
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阳性对照
- Melanoma.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: Tissue culture supernatant, 1% BSA -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
PNL2 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab12502于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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相关性
Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre existing benign nevus, which occurs most often in the skin but also may involve other sites. -
细胞定位
Membrane; Single-pass membrane protein -
数据库链接
- Omim: 605513 Human
- SwissProt: Q16655 Human
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别名
- Antigen LB39 AA antibody
- Antigen SK29 AA antibody
- MART1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [PNL2] (ab12502)IHC image of ab12502 staining in human melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab12502, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [PNL2] (ab12502)](https://www.abcam.cn/ps/products/12/ab12502/Images/ab12502-295035-anti-melanoma-antibody-pnl2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [PNL2] (ab12502)Formalin-fixed, paraffin-embedded human melanoma tissue stained for Melanoma using ab12502 at 1/50 dilution in immunohistochemical analysis.
数据表及文件
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Datasheet download
文献 (4)
ab12502 被引用在 4 文献中.
- Moroki T et al. Databases for technical aspects of immunohistochemistry: 2021 update. J Toxicol Pathol 34:161-180 (2021). PubMed: 33976473
- Needle DB et al. Biphasic malignant melanoma adenocarcinoma in the digit of a dog. J Vet Diagn Invest 30:315-318 (2018). PubMed: 29284381
- Furukawa S et al. Databases for technical aspects of immunohistochemistry. J Toxicol Pathol 30:79-107 (2017). IHC ; Rat . PubMed: 28190929
- Rochaix P et al. PNL2, a new monoclonal antibody directed against a fixative-resistant melanocyte antigen. Mod Pathol 16:481-90 (2003). PubMed: 12748255
