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AB12502

Anti-Melanoma抗体[PNL2]

Anti-Melanoma antibody [PNL2]

4

(1 Review)

|

(4 Publications)

Mouse Monoclonal Melanoma antibody. Suitable for IHC-P and reacts with Human samples. Cited in 4 publications.

查看别名

Antigen LB39-AA, Antigen SK29-AA, MART-1, Melan A, Melan A protein, Melanoma antigen recognized by T-cells 1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [PNL2] (AB12502)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [PNL2] (AB12502)

IHC image of ab12502 staining in human melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab12502, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [PNL2] (AB12502)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [PNL2] (AB12502)

Formalin-fixed, paraffin-embedded human melanoma tissue stained for Melanoma using ab12502 at 1/50 dilution in immunohistochemical analysis.

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

PNL2

亚型

IgG1

不含载体蛋白

No

反应种属

Human

应用

IHC-P

applications

特异性

This antibody labels melanocytes in the cytoplasm and plasma membrane and is useful tool for the identification of melanomas and clear cell sarcomas. It seems to be a melanocyte antibody of autoimmune origin as it does not react with the non-melanocytic peptide antigen used for immunization.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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性能和储存信息

形式
Liquid
纯度
Tissue culture supernatant
存储溶液
pH: 7.3 - 7.5 Preservative: 0.05% Sodium azide Constituents: Tissue culture supernatant, 1% BSA
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

See full target information Melanoma
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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of toxicologic pathology 34:161-180 PubMed33976473

2021

Databases for technical aspects of immunohistochemistry: 2021 update.

Applications

Unspecified application

Species

Unspecified reactive species

Takayasu Moroki,Saori Matsuo,Hirofumi Hatakeyama,Seigo Hayashi,Izumi Matsumoto,Satoshi Suzuki,Takashi Kotera,Kazuyoshi Kumagai,Kiyokazu Ozaki

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc 30:315-318 PubMed29284381

2017

Biphasic malignant melanoma adenocarcinoma in the digit of a dog.

Applications

Unspecified application

Species

Unspecified reactive species

David B Needle,Olga Iglikova,Andrew D Miller

Journal of toxicologic pathology 30:79-107 PubMed28190929

2017

Databases for technical aspects of immunohistochemistry.

Applications

IHC

Species

Rat

Satoshi Furukawa,Mika Nagaike,Kiyokazu Ozaki

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 16:481-90 PubMed12748255

2003

PNL2, a new monoclonal antibody directed against a fixative-resistant melanocyte antigen.

Applications

Unspecified application

Species

Unspecified reactive species

Philippe Rochaix,Magali Lacroix-Triki,Laurence Lamant,Carole Pichereaux,Severine Valmary,Elena Puente,Talal Al Saati,Bernard Monsarrat,Christiane Susini,Louis Buscail,Georges Delsol,Jean-Jacques Voigt
View all publications
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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
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