重组Anti-Melanoma抗体[HMB45 + M2-7C10 + M2-9E3] (ab732)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse recombinant multiclonal [HMB45 + M2-7C10 + M2-9E3] to Melanoma
- Suitable for: IHC-Fr, IHC-P, Flow Cyt
- Reacts with: Human
概述
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产品名称
Anti-Melanoma抗体[HMB45 + M2-7C10 + M2-9E3]
参阅全部 Melanoma 一抗 -
描述
小鼠重组multiclonal [HMB45 + M2-7C10 + M2-9E3] to Melanoma -
宿主
Mouse -
经测试应用
适用于: IHC-Fr, IHC-P, Flow Cytmore details -
种属反应性
与反应: Human
预测可用于: Rat -
免疫原
Recombinant full length protein corresponding to Melanoma. HMB45 - Pigmented melanoma metastases from LN MART-1 - Recombinant human MART-1 protein
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表位
HMB45 and MART-1 -
阳性对照
- IHC-P: Metastatic melanoma in lymph node.
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常规说明
This cocktail is HMB45 + two different clones of MART-1[M2-7C10] + [M2-9E3]. HMB34 isotype: IgG1/kappa and both clones of MART-1: IgG2b + IgG2b
HMB-45 and MART-1 are coexpressed in the majority of melanomas, as well as uniquely expressed in certain cases. Thus, the HMB-45 and MART-1 cocktail is potentially more sensitive than HMB-45 or MART-1 alone. MART-1 is a cocktail of clones M2-7C10 and M2-9E3. The combination of HMB-45 and the MART-1 cocktail make this triple antibody cocktail a first-order pan melanoma screener.
This product was changed from ascites to tissue culture supernatant on 14/07/17. The following lots are from ascites and are still in stock as of 14/07/17 – GR313023 and GR3175007. Lot numbers higher than GR313023 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.099% Sodium azide
Constituents: 0.9% Proprietary component, 99% Water -
Concentration information loading...
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纯度
Tissue culture supernatant -
Primary antibody说明
HMB-45 and MART-1 are coexpressed in the majority of melanomas, as well as uniquely expressed in certain cases. Thus, the HMB-45 and MART-1 cocktail is potentially more sensitive than HMB-45 or MART-1 alone. MART-1 is a cocktail of clones DT101 and BC199. The combination of HMB-45 and the MART-1 cocktail make this triple antibody cocktail a first-order pan melanoma screener. -
克隆
Recombinant Multiclonal -
克隆编号
HMB45 + M2-7C10 + M2-9E3 -
骨髓瘤
unknown -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
应用 | Ab评论 | 说明 |
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IHC-Fr |
Use at an assay dependent concentration.
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IHC-P | (1) |
1/100 - 1/200.
The staining intensity of this melanoma cocktail is enhanced by performing heat mediated antigen retrieval using a high pH buffer. The HMB-45 portion of this antibody will stain with citrate buffer, pH 6.0; however, data shows the MART-1 portion of this cocktail may be negative unless a high pH buffer is used. We therefore, strongly recommend that you do not use citrate buffer for this antibody cocktail. |
Flow Cyt |
Use at an assay dependent concentration. PubMed: 22147606
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
说明 |
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IHC-Fr
Use at an assay dependent concentration. |
IHC-P
1/100 - 1/200. The staining intensity of this melanoma cocktail is enhanced by performing heat mediated antigen retrieval using a high pH buffer. The HMB-45 portion of this antibody will stain with citrate buffer, pH 6.0; however, data shows the MART-1 portion of this cocktail may be negative unless a high pH buffer is used. We therefore, strongly recommend that you do not use citrate buffer for this antibody cocktail. |
Flow Cyt
Use at an assay dependent concentration. PubMed: 22147606 ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
靶标
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相关性
Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre existing benign nevus, which occurs most often in the skin but also may involve other sites. -
细胞定位
Membrane; Single-pass membrane protein -
数据库链接
- Entrez Gene: 293890 Rat
- Omim: 605513 Human
- SwissProt: Q16655 Human
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别名
- Antigen LB39 AA antibody
- Antigen SK29 AA antibody
- MART1 antibody
see all
图片
数据表及文件
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Datasheet download
文献 (23)
ab732 被引用在 23 文献中.
- Netanely D et al. Classification of node-positive melanomas into prognostic subgroups using keratin, immune, and melanogenesis expression patterns. Oncogene 40:1792-1805 (2021). PubMed: 33564068
- Lee RJ et al. Brain microenvironment-driven resistance to immune and targeted therapies in acral melanoma. ESMO Open 5:N/A (2020). PubMed: 32817058
- Golan T et al. Adipocytes sensitize melanoma cells to environmental TGF-ß cues by repressing the expression of miR-211. Sci Signal 12:N/A (2019). PubMed: 31337739
- Li H et al. YAP/TAZ Activation Drives Uveal Melanoma Initiation and Progression. Cell Rep 29:3200-3211.e4 (2019). PubMed: 31801083
- Han N et al. Enhanced IL-34 expression in Nivolumab-resistant metastatic melanoma. Inflamm Regen 38:3 (2018). PubMed: 29515691