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Anti-Melanocyte cell surface antigen抗体[Mel.2] (ab128759)

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Key features and details

  • Mouse monoclonal [Mel.2] to Melanocyte cell surface antigen
  • Suitable for: IHC-Fr, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-Melanocyte cell surface antigen抗体[Mel.2]

  • 描述

    小鼠单克隆抗体[Mel.2] to Melanocyte cell surface antigen

  • 宿主

    Mouse

  • 特异性

    ab128759 recognizes Melanocyte cell surface antigen expressed exclusively by Human melanocytes and pigmented Human tumours.

  • 经测试应用

    适用于: IHC-Fr, ICC/IFmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Tissue, cells or virus corresponding to Human Melanocyte cell surface antigen. Human KW mel (2.1) melanoma cells.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

  • 存储溶液

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: 98% PBS
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 纯化说明

    Purified IgG prepared by affinity chromatography on Protein A

  • 克隆

    单克隆

  • 克隆编号

    Mel.2

  • 同种型

    IgG1

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab128759于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
说明
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.

靶标

  • 相关性

    It has been reported that the pathogenesis of melanoma proceeds through multiple stages, ranging from benign proliferation of melanocytic cells to acquisition of the capacity to invade tissues and metastasise. Cell surface antigens that are expressed by melanocytes are not expressed by melanoma cell lines.

  • 细胞定位

    Cell Membrane

文献 (0)

发表研究结果有使用 ab128759？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab128759 尚未被引用在任何文献中。

客户评价及客户问答

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1-3 of 3 Abreviews or Q&A

Question

Thanks very much for your email and protocol. You see, I talked about your Mel.2 Ab with my boss for our Dynabeads experiment. We are really interested in this Ab, since they are targeting the outside of the melanocytes. However, there is no any reference about relevant study at this point, it would be reasonable to send us a little bit Ab as a sample for letting us to try it on. If our experiment is going well, It could be served as your future reference.

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Answer

Thank you very much for your interest in ab128759. I will be helping you while Tom is out of the office.

We do not currently have an image of ab128759 in ICC/IF and we are unable to send small aliquots because we cannot store multple vial sizes in our automated freezers.

However by participating in our AbTrial program you can now use our products without financial risk.

Simply follow these easy steps below to apply for our AbTrial Program:

1. Reply to this email, letting us know you are interested in testing this product.

2. Our scientists will email you an inactive personal discount code for the value of the product.

3. Purchase and test the product at the regular price.

4. Submit an image of your results, including your discount code in the additional notes section of your Abreview.

5. Once the Abreview is submitted, the discount code will become active.

6. Apply your discount code on your next order to receive that value off.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: https://www.abcam.com/abtrial.

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Question

Is it known if the epitope this antibody recognizes is extracellular? We are hoping to isolate a population of live melanocytes. We will first validate the antibody by ICC. Please send the ICC protocol.

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Answer

We have not performed any epitope mapping with this antibody. While it is known that the antibody is localized in or on the cell plasma membrane, but we do not know the specific region of binding. This antibody has been successfully tested for use in ELISA using fresh melanoma cells and in IHC-frozen at 8um thin sections. Successful fluorescence staining of melanoma cells on glass has been observed after being, washed, fixed for 30 seconds using ice cold acetone, and air dried. This is the extent of the protocol information we have available to share from localization experiments. I have included our ICC protocol with this email.

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Question

Is it known if the epitope this antibody recognizes is extracellular? We are hoping to isolate a population of live melanocytes. We will first validate the antibody by ICC. Please send the ICC protocol.

Read More
Answer

Unfortunately we have not performed any epitope mapping with this antibody. We know that the epitope is localized in or on the cell plasma membrane, but we do not know the specific region of binding.

This antibody has been successfully tested for use in ELISA using fresh melanoma cells and in IHC-frozen at 8um thin sections. Successful fluorescence staining of melanoma cells on glass has been observed by an outside laboratory after being, washed, fixed for 30 seconds using ice cold acetone, and air dried. This is the extent of the protocol information we have available to share from localization experiments.

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