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Tags & Cell Markers Cell Type Markers Tumor Associated
重组

重组Anti-MelanA抗体[DT101 + BC199] (ab731)

  • Datasheet
Reviews (3)Q&A (1)References (15)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [DT101 + BC199] (ab731)

    Key features and details

    • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
    • Mouse recombinant multiclonal [DT101 + BC199] to MelanA
    • Suitable for: IHC-P, IHC-Fr
    • Reacts with: Mouse, Rat, Dog, Human

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    概述

    • 产品名称

      Anti-MelanA抗体[DT101 + BC199]
      参阅全部 MelanA 一抗
    • 描述

      小鼠重组multiclonal [DT101 + BC199] to MelanA
    • 宿主

      Mouse
    • 经测试应用

      适用于: IHC-P, IHC-Frmore details
    • 种属反应性

      与反应: Mouse, Rat, Dog, Human
    • 免疫原

      Recombinant full length protein corresponding to Human MelanA.

    • 阳性对照

      • Melanoma
    • 常规说明

      This product was changed from ascites to tissue culture supernatant on 8th March 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

      Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      Constituents: PBS, Tissue culture supernatant

      Proprietary preservative that is not sodium azide or thimerosal, protein carrier.
    • Concentration information loading...
    • 纯度

      Tissue culture supernatant
    • 克隆

      Recombinant Multiclonal
    • 克隆编号

      DT101 + BC199
    • 骨髓瘤

      unknown
    • 同种型

      IgG2b
    • 轻链类型

      unknown
    • 研究领域

      • Tags & Cell Markers
      • Cell Type Markers
      • Tumor Associated
      • Cancer
      • Tumor immunology
      • Tumor-associated antigens

    相关产品

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
    • Recombinant Protein

      • Recombinant Human MelanA protein (ab114312)
      • Recombinant Human MelanA protein (ab157272)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab731于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P (2)
    1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC method.
    IHC-Fr
    Use at an assay dependent concentration.

    ABC method.It is sometimes difficult to interpret DAB stained melanomas due to endogenous pigment.  We recommend you substitute an AEC or a Fast Red substrate protocol.  An avidin biotin blocking may be required for certain types of melanomas.

    说明
    IHC-P
    1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC method.
    IHC-Fr
    Use at an assay dependent concentration.

    ABC method.It is sometimes difficult to interpret DAB stained melanomas due to endogenous pigment.  We recommend you substitute an AEC or a Fast Red substrate protocol.  An avidin biotin blocking may be required for certain types of melanomas.

    靶标

    • 功能

      Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.
    • 组织特异性

      Expression is restricted to melanoma and melanocyte cell lines and retina.
    • 翻译后修饰

      Acylated.
    • 细胞定位

      Endoplasmic reticulum membrane. Golgi apparatus. Golgi apparatus > trans-Golgi network membrane. Melanosome. Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post-Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation.
    • Target information above from: UniProt accession Q16655 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 403495 Dog
      • Entrez Gene: 2315 Human
      • Entrez Gene: 77836 Mouse
      • Entrez Gene: 293890 Rat
      • Omim: 605513 Human
      • SwissProt: Q16655 Human
      • Unigene: 154069 Human
      • 别名

        • Antigen LB39 AA antibody
        • Antigen LB39-AA antibody
        • Antigen SK29 AA antibody
        • Antigen SK29-AA antibody
        • MAR1_HUMAN antibody
        • MART 1 antibody
        • MART-1 antibody
        • MART1 antibody
        • Melan A antibody
        • Melan A protein antibody
        • Melanoma antigen recognized by T cells 1 antibody
        • Melanoma antigen recognized by T-cells 1 antibody
        • MLAN A antibody
        • MLANA antibody
        • OTTHUMP00000021036 antibody
        • OTTHUMP00000021037 antibody
        • OTTHUMP00000021038 antibody
        • Protein Melan-A antibody
        see all

      图片

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [DT101 + BC199] (ab731)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [DT101 + BC199] (ab731)

        Immunohistochemistry analysis of Melanoma tissue labeling MelanA with ab731.

      实验方案

      • Immunohistochemistry protocols

      Click here to view the general protocols

      数据表及文件

      • Datasheet download

        Download

      文献 (15)

      发表研究结果有使用 ab731?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab731 被引用在 15 文献中.

      • Jeong YM  et al. 3D-Printed Collagen Scaffolds Promote Maintenance of Cryopreserved Patients-Derived Melanoma Explants. Cells 10:N/A (2021). PubMed: 33800001
      • Ishibashi T  et al. Methyl-CpG binding domain protein 3: a new diagnostic marker and potential therapeutic target of melanoma. Biosci Trends 14:390-395 (2020). PubMed: 32963182
      • Semba T  et al. Periostin antisense oligonucleotide suppresses bleomycin-induced formation of a lung premetastatic niche for melanoma. Cancer Sci 109:1447-1454 (2018). PubMed: 29498146
      • Zeng H  et al. Bi-allelic Loss of CDKN2A Initiates Melanoma Invasion via BRN2 Activation. Cancer Cell 34:56-68.e9 (2018). PubMed: 29990501
      • Cao J  et al. Tuberous sclerosis complex inactivation disrupts melanogenesis via mTORC1 activation. J Clin Invest 127:349-364 (2017). PubMed: 27918305
      View all Publications for this product

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-4 of 4 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-MelanA antibody [DT101 + BC199]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (Skin, melanocytes)
      Permeabilization
      No
      Specification
      Skin, melanocytes
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Mar 27 2019

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MelanA antibody [DT101 + BC199]

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Blocking step
      BSA as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: citrate buffer, pH6.0
      Sample
      Human Tissue sections (adult skin)
      Specification
      adult skin
      Permeabilization
      Yes - Tween-20
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Mr. Heiko Locher

      Verified customer

      提交于 Sep 20 2013

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MelanA antibody [DT101 + BC199]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (lymph node)
      Specification
      lymph node
      Fixative
      Acetone
      Antigen retrieval step
      None
      Permeabilization
      No
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 May 01 2009

      Question

      Hello, I wondered if you could help me please. I am looking to stain human biopsy tissue sections taken from melanoma tumours. I am interested in tumour antigens gp100, MART-1 and tyrosinase. Do you have any antibodies to these tumour antigens suitable for immunohistochemistry use? The biopsy tissue sections can be formalin fixed or frozen. I notice that you have several MART-1 antibodies, could you recommend which may be best for my purposes. Could you also forward a protocol or the method, if possible, and let me know whether I will require a secondary Ab, and if so which you would recommend. Kind Regards, Claire

      Read More

      Abcam community

      Verified customer

      Asked on Nov 20 2003

      Answer

      I suggest that you conduct a search through the Abcam Website. I also recommend that you should click on the links “Product Reviews” and “Technical Enquiries” both accessible via the green menu bar on the datasheet. Here you will find customer reviews and past questions and answers specifically relating to that product. If you cannot find what you are looking for within the Abcam catalog, I suggest that you click on the link to "The World's Antibody Gateway". The World's Antibody Gateway is a free search engine service provided by Abcam to help you to quickly find the antibodies that you are looking for. It is a free-text search engine developed by the Abcam team so that it searches the catalogs of all online antibody companies (currently 249).

      Read More

      Abcam Scientific Support

      回复于 Nov 20 2003

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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