重组Anti-MelanA抗体[DT101 + BC199] (ab731)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [DT101 + BC199] (ab731)](https://www.abcam.cn/ps/products/0/ab731/Images/ab731-205305-anti-melana-antibody-dt101-bc199-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [DT101 + BC199] (ab731)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse recombinant multiclonal [DT101 + BC199] to MelanA
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Dog, Human
概述
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产品名称
Anti-MelanA抗体[DT101 + BC199]
参阅全部 MelanA 一抗 -
描述
小鼠重组multiclonal [DT101 + BC199] to MelanA -
宿主
Mouse -
经测试应用
适用于: IHC-P, IHC-Frmore details -
种属反应性
与反应: Mouse, Rat, Dog, Human -
免疫原
Recombinant full length protein corresponding to Human MelanA.
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阳性对照
- Melanoma
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常规说明
This product was changed from ascites to tissue culture supernatant on 8th March 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituents: PBS, Tissue culture supernatant
Proprietary preservative that is not sodium azide or thimerosal, protein carrier. -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
Recombinant Multiclonal -
克隆编号
DT101 + BC199 -
骨髓瘤
unknown -
同种型
IgG2b -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (2) |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC method.
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| IHC-Fr |
Use at an assay dependent concentration.
ABC method.It is sometimes difficult to interpret DAB stained melanomas due to endogenous pigment. We recommend you substitute an AEC or a Fast Red substrate protocol. An avidin biotin blocking may be required for certain types of melanomas. |
| 说明 |
|---|
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC method. |
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IHC-Fr
Use at an assay dependent concentration. ABC method.It is sometimes difficult to interpret DAB stained melanomas due to endogenous pigment. We recommend you substitute an AEC or a Fast Red substrate protocol. An avidin biotin blocking may be required for certain types of melanomas. |
靶标
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功能
Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes. -
组织特异性
Expression is restricted to melanoma and melanocyte cell lines and retina. -
翻译后修饰
Acylated. -
细胞定位
Endoplasmic reticulum membrane. Golgi apparatus. Golgi apparatus > trans-Golgi network membrane. Melanosome. Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post-Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation. - Information by UniProt
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数据库链接
- Entrez Gene: 403495 Dog
- Entrez Gene: 2315 Human
- Entrez Gene: 77836 Mouse
- Entrez Gene: 293890 Rat
- Omim: 605513 Human
- SwissProt: Q16655 Human
- Unigene: 154069 Human
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别名
- Antigen LB39 AA antibody
- Antigen LB39-AA antibody
- Antigen SK29 AA antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [DT101 + BC199] (ab731)
Immunohistochemistry analysis of Melanoma tissue labeling MelanA with ab731.
数据表及文件
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Datasheet download
文献 (15)
ab731 被引用在 15 文献中.
- Jeong YM et al. 3D-Printed Collagen Scaffolds Promote Maintenance of Cryopreserved Patients-Derived Melanoma Explants. Cells 10:N/A (2021). PubMed: 33800001
- Ishibashi T et al. Methyl-CpG binding domain protein 3: a new diagnostic marker and potential therapeutic target of melanoma. Biosci Trends 14:390-395 (2020). PubMed: 32963182
- Semba T et al. Periostin antisense oligonucleotide suppresses bleomycin-induced formation of a lung premetastatic niche for melanoma. Cancer Sci 109:1447-1454 (2018). PubMed: 29498146
- Zeng H et al. Bi-allelic Loss of CDKN2A Initiates Melanoma Invasion via BRN2 Activation. Cancer Cell 34:56-68.e9 (2018). PubMed: 29990501
- Cao J et al. Tuberous sclerosis complex inactivation disrupts melanogenesis via mTORC1 activation. J Clin Invest 127:349-364 (2017). PubMed: 27918305
