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AB785

Anti-MelanA 抗体 [A103]

Anti-MelanA antibody [A103]

5

(1 Review)

|

(4 Publications)

Mouse Monoclonal MelanA antibody. Suitable for IHC-P, Flow Cyt, IHC-Fr and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MLANA.

查看别名

MART1, MLANA, Melanoma antigen recognized by T-cells 1, MART-1, Antigen LB39-AA, Antigen SK29-AA, Protein Melan-A

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [A103] (AB785)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [A103] (AB785)

IHC image of ab785 staining in Human Skin Melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab785, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [A103] (AB785)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [A103] (AB785)

Formalin-fixed, paraffin-embeded human melanoma tissue stained for Melan A using ab785 at 1/50 dilution in immunohistochemical analysis. Antigen retrieval with citrate buffer pH 6.0

Flow Cytometry - Anti-MelanA antibody [A103] (AB785)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-MelanA antibody [A103] (AB785)

Overlay histogram showing Malme-3 cells stained with ab785 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab785, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Malme-3 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

A103

亚型

IgG1

轻链类型

unknown

不含载体蛋白

No

反应种属

Human

应用

Flow Cyt, IHC-Fr, IHC-P

applications

免疫原

Recombinant Full Length Protein corresponding to Human MLANA.

Q16655

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
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您是否考虑以下替代产品？

AB210546

Anti-MelanA antibody [EPR20380]

5
15 Reviews
查看产品
为什么推荐这个？

为向您提供最高品质的产品，我们在不断升级产品范围。我们选择了这个替代产品供您参考，因为它提供了更好的性能，更容易和更方便的使用，或交期更快。

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性能和储存信息

形式
Liquid
纯度
Tissue culture supernatant
存储溶液
pH: 7.3 - 7.5 Preservative: 0.05% Sodium azide Constituents: 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
储存信息
Avoid freeze / thaw cycle
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.
See full target information MLANA
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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell 34:69-84.e14 PubMed30008323

2018

EZH2-Mediated Primary Cilium Deconstruction Drives Metastatic Melanoma Formation.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Zingg,Julien Debbache,Rodrigo Peña-Hernández,Ana T Antunes,Simon M Schaefer,Phil F Cheng,Dario Zimmerli,Jessica Haeusel,Raquel R Calçada,Eylul Tuncer,Yudong Zhang,Raphaël Bossart,Kwok-Kin Wong,Konrad Basler,Reinhard Dummer,Raffaella Santoro,Mitchell P Levesque,Lukas Sommer

Veterinary pathology 52:456-64 PubMed25161210

2014

The origin and role of autophagy in the formation of cytoplasmic granules in canine lingual granular cell tumors.

Applications

Unspecified application

Species

Dog

S Suzuki,K Uchida,T Harada,K Nibe,M Yamashita,K Ono,H Nakayama

Cancer research 71:3098-109 PubMed21393506

2011

Human CD271-positive melanoma stem cells associated with metastasis establish tumor heterogeneity and long-term growth.

Applications

IHC-P

Species

Human

Gianluca Civenni,Anne Walter,Nikita Kobert,Daniela Mihic-Probst,Marie Zipser,Benedetta Belloni,Burkhardt Seifert,Holger Moch,Reinhard Dummer,Maries van den Broek,Lukas Sommer

Proceedings of the National Academy of Sciences of the United States of America 93:5915-9 PubMed8650193

1996

Serological analysis of Melan-A(MART-1), a melanocyte-specific protein homogeneously expressed in human melanomas.

Applications

Unspecified application

Species

Unspecified reactive species

Y T Chen,E Stockert,A Jungbluth,S Tsang,K A Coplan,M J Scanlan,L J Old
View all publications
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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
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关联产品

Select an associated product type
Alternative Product
Primary Antibodies

AB225499

PE Anti-MelanA antibody [EPR20380]

primary-antibodies

pe-melana-antibody-epr20380-ab225499

0

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