AB239826

重组Anti-MEKK3抗体[ep600y] - BSA and Azide free

Anti-MEKK3 antibody [ep600y] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal MEKK3 antibody. Carrier free. Suitable for IHC-P, IP, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 1 publication.

查看别名

MAPKKK3, MEKK3, MAP3K3, Mitogen-activated protein kinase kinase kinase 3, MAPK/ERK kinase kinase 3, MEK kinase 3, MEKK 3

5 Images

Flow Cytometry (Intracellular) - Anti-MEKK3 antibody [ep600y] - BSA and Azide free (AB239826)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-MEKK3 antibody [ep600y] - BSA and Azide free (AB239826)

This data was developed using ab40756, the same antibody clone in a different buffer formulation.

Flow Cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling MEKK3 with purified ab40756 at 1/100 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEKK3 antibody [ep600y] - BSA and Azide free (AB239826)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEKK3 antibody [ep600y] - BSA and Azide free (AB239826)

Immunohistochemical analysis of paraffin-embedded human ovary carcinoma using ab40756 diluted 1 : 100

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40756).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-MEKK3 antibody [ep600y] - BSA and Azide free (AB239826)

This data was developed using ab40756, the same antibody clone in a different buffer formulation.

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling MEKK3 with purified ab40756 at 1/1000 dilution (1 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunocytochemistry/ Immunofluorescence - Anti-MEKK3 antibody [ep600y] - BSA and Azide free (AB239826)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MEKK3 antibody [ep600y] - BSA and Azide free (AB239826)

Immunofluorescent staining of HeLa cells using ab40756 diluted 1 : 100This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40756).

Lab

Immunoprecipitation - Anti-MEKK3 antibody [ep600y] - BSA and Azide free (AB239826)

71 kDa MEKK3 was immunoprecipitated from 0.35mg of bEnd.3 (mouse brain endothelial cell) whole cell lysate with ab40756 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab40756 at 1/200 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution. Lane 1 (Input) : bEnd.3 (mouse brain endothelial cell) whole cell lysate 10 μg. Lane 2 (+) : bEnd.3 whole cell lysate. Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab40756 in bEnd.3 whole cell lysate Blocking buffer and concentration : 5% NFDM/TBST. Diluting buffer and concentration : 5% NFDM/TBST. Exposure time : 180 seconds. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40756).

All lanes:

Immunoprecipitation - Anti-MEKK3 antibody [ep600y] (<a href='/products/primary-antibodies/mekk3-antibody-ep600y-ab40756'>ab40756</a>) at 1/200 dilution

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Predicted band size: 71 kDa

Observed band size: 71 kDa

false

Exposure time: 180s

不同偶联物与剂型 (1)

Unconjugated

Anti-MEKK3 antibody [ep600y]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

ep600y

亚型

IgG

不含载体蛋白

Yes

反应种属

Human, Mouse

应用

ICC/IF, Flow Cyt (Intra), IP, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-low-endotoxin-azide-free-ab199376'>ab199376</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

ab239826 is the carrier-free version of ab40756.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MEKK3 also known as MAP3K3 is a serine/threonine kinase that plays an important role in the MAPK signaling cascade. This protein has a molecular mass of approximately 67 kDa. MEKK3 is widely expressed with notable presence in tissues like the heart placenta and skeletal muscles. It is important for transmitting signals from the cell surface to various intracellular targets impacting numerous cellular processes.

Biological function summary

MEKK3 participates in cell growth differentiation and apoptosis regulation. It often functions within a multi-protein complex including components like MEK5 and ERK5. MEKK3 activates downstream kinases influencing gene expression and cellular responses to external signals. Its involvement is essential for controlling reactions to stress and inflammatory stimuli.

Pathways

MEKK3 integrates into the MAPK and NF-κB pathways both critical for mediating signaling responses. In the MAPK pathway MEKK3 regulates kinases like MEK5 and ERK5. Additionally it connects with proteins such as IKK in the NF-κB pathway influencing immunity and cell survival. These interactions position MEKK3 centrally in cellular defense and adaptability mechanisms.

MEKK3 associates with pathological conditions like cardiovascular diseases and cancer. Its role in these disorders links closely with dysregulation in the MAPK pathway where MEK5 and ERK5 also play significant roles. Furthermore aberrations in the NF-κB pathway involving IKK can contribute to its involvement in these diseases highlighting MEKK3's impact on disease progression and potential as a therapeutic target.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators.

See full target information MAP3K3

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 44:1145-1162 PubMed38235747

2024

The role of serum/glucocorticoid-regulated kinase 1 in brain function following cerebral ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

Celeste Yin-Chieh Wu,Yulan Zhang,Li Xu,Zhihai Huang,Peibin Zou,Garrett A Clemons,Chun Li,Cristiane T Citadin,Quanguang Zhang,Reggie Hui-Chao Lee

View all publications

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Abcam Product Promise

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详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-MEKK3抗体[ep600y] - BSA and Azide free