重组Anti-MEK4/MKK4 (phospho S80)抗体[EP1075Y] (ab52958)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK4/MKK4 (phospho S80) antibody [EP1075Y] (ab52958)](https://www.abcam.cn/ps/products/52/ab52958/Images/ab52958-65511-anti-mek4-phospho-s80-antibody-ep1075y-immunohistochemistry.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK4/MKK4 (phospho S80) antibody [EP1075Y] (ab52958)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1075Y] to MEK4/MKK4 (phospho S80)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-MEK4/MKK4 (phospho S80)抗体[EP1075Y]
参阅全部 MEK4/MKK4 一抗 -
描述
兔单克隆抗体[EP1075Y] to MEK4/MKK4 (phospho S80) -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: 3T3 cell lysate treated with PDGF. IHC-P: Human muscle tissue.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1075Y -
同种型
IgG -
研究领域
相关产品
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52958于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
|
| WB | (1) |
1/5000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
|
| 说明 |
|---|
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
WB
1/5000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). |
靶标
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功能
Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1). -
组织特异性
Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues. -
序列相似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases. - Information by UniProt
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数据库链接
- Entrez Gene: 6416 Human
- Entrez Gene: 26398 Mouse
- Omim: 601335 Human
- SwissProt: P45985 Human
- SwissProt: P47809 Mouse
- Unigene: 514681 Human
- Unigene: 412922 Mouse
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别名
- c Jun N terminal kinase kinase 1 antibody
- C-JUN N-terminal kinase kinase 1 antibody
- Dual specificity mitogen activated protein kinase kinase 4 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK4/MKK4 (phospho S80) antibody [EP1075Y] (ab52958)
ab52958 at 1/50 dilution staining MEK4/MKK4 in human muscles by Immunohistochemistry, Paraffin embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-MEK4/MKK4 (phospho S80) antibody [EP1075Y] (ab52958)](https://www.abcam.cn/ps/products/52/ab52958/Images/ab52958-65512-anti-mek4-phospho-s80-antibody-ep1075y-western-blot.gif)
Western blot - Anti-MEK4/MKK4 (phospho S80) antibody [EP1075Y] (ab52958)All lanes : Anti-MEK4/MKK4 (phospho S80) antibody [EP1075Y] (ab52958) at 1/5000 dilution
Lane 1 : 3T3 cell lysate untreated
Lane 2 : 3T3 cell lysate treated with PDGF
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab52958 被引用在 2 文献中.
- Yu W et al. TRPV3 inhibits colorectal cancer cell proliferation and migration by regulating the MAPK signaling pathway. J Gastrointest Oncol 13:2447-2457 (2022). PubMed: 36388700
- Wang J et al. Inhibition of ASC enhances the protective role of salvianolic acid A in traumatic brain injury via inhibition of inflammation and recovery of mitochondrial function. Folia Neuropathol 59:50-66 (2021). PubMed: 33969677