Anti-MEK4/MKK4 (phospho S80)抗体(ab131353)
Key features and details
- Rabbit polyclonal to MEK4/MKK4 (phospho S80)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-MEK4/MKK4 (phospho S80)抗体
参阅全部 MEK4/MKK4 一抗 -
描述
兔多克隆抗体to MEK4/MKK4 (phospho S80) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human MEK4/MKK4 (internal sequence) (phospho S80) conjugated to keyhole limpet haemocyanin.
Database link: NP_003001.1 -
阳性对照
- Methanol fixed HeLa cells; HepG2 cell extracts, treated with EGF; Human breast carcinoma tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS (without Mg2+and Ca2+) -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab131353 was purified by using epitope specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131353于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
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| IHC-P |
1/50 - 1/100.
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| ICC/IF |
1/100 - 1/200.
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| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). |
|
IHC-P
1/50 - 1/100. |
|
ICC/IF
1/100 - 1/200. |
靶标
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功能
Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1). -
组织特异性
Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues. -
序列相似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases. - Information by UniProt
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数据库链接
- Entrez Gene: 6416 Human
- Omim: 601335 Human
- SwissProt: P45985 Human
- Unigene: 514681 Human
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别名
- c Jun N terminal kinase kinase 1 antibody
- C-JUN N-terminal kinase kinase 1 antibody
- Dual specificity mitogen activated protein kinase kinase 4 antibody
see all
图片
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Western blot - Anti-MEK4/MKK4 (phospho S80) antibody (ab131353)All lanes : Anti-MEK4/MKK4 (phospho S80) antibody (ab131353) at 1/500 dilution
Lane 1 : HepG2 cell extracts, untreated
Lane 2 : HepG2 cell extracts, treated with EGF
Predicted band size: 44 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-MEK4/MKK4 (phospho S80) antibody (ab131353)Immunofluorescent analysis of methanol fixed HeLa cells labelling MEK4/MKK4 (phospho S80) with ab131353 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK4/MKK4 (phospho S80) antibody (ab131353)Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling MEK4/MKK4 (phospho S80) with ab131353 at 1/50 dilution (left). The image on the right is treated with blocking peptide.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab131353 被引用在 3 文献中.
- Lu Z et al. PP2A protects podocytes against Adriamycin-induced injury and epithelial-to-mesenchymal transition via suppressing JIP4/p38-MAPK pathway. Cytotechnology 73:697-713 (2021). PubMed: 34629746
- Wu X et al. Amyloid precursor protein promotes the migration and invasion of breast cancer cells by regulating the MAPK signaling pathway. Int J Mol Med 45:162-174 (2020). PubMed: 31746365
- Tang Y et al. Electroacupuncture Ameliorates Cognitive Impairment by Inhibiting the JNK Signaling Pathway in a Mouse Model of Alzheimer's Disease. Front Aging Neurosci 12:23 (2020). PubMed: 32116652