Anti-MEK4/MKK4抗体(ab131494)
Key features and details
- Rabbit polyclonal to MEK4/MKK4
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-MEK4/MKK4抗体
参阅全部 MEK4/MKK4 一抗 -
描述
兔多克隆抗体to MEK4/MKK4 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human MEK4/MKK4 aa 259-263 (internal sequence) conjugated to keyhole limpet haemocyanin.
Database link: NP_003001.1 -
阳性对照
- Human breast carcinoma tissue
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS is without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab131494 was purified by affinity chromatography using an epitope specific phosphopeptide. Non phosphopeptide specific antibodies were removed by chromatography using non phosphopeptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131494于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/50 - 1/100.
|
|
| ICC/IF |
1/100 - 1/200. (methanol fixed cells)
|
| 说明 |
|---|
|
IHC-P
1/50 - 1/100. |
|
ICC/IF
1/100 - 1/200. (methanol fixed cells) |
靶标
-
功能
Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1). -
组织特异性
Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues. -
序列相似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases. - Information by UniProt
-
数据库链接
- Entrez Gene: 6416 Human
- Omim: 601335 Human
- SwissProt: P45985 Human
- Unigene: 514681 Human
-
别名
- c Jun N terminal kinase kinase 1 antibody
- C-JUN N-terminal kinase kinase 1 antibody
- Dual specificity mitogen activated protein kinase kinase 4 antibody
see all
图片
-
Immunocytochemistry/ Immunofluorescence - Anti-MEK4/MKK4 antibody (ab131494)
Immunofluorescence analysis of MEK4/MKK4 in methanol fixed HeLa cells stained with ab131494 at a 1/100 dilution.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK4/MKK4 antibody (ab131494)Immunohistochemical analysis of MEK4/MKK4 in paraffin embedded Human breast cancer tissue, using ab131494 at a 1/50 dilution. The image on the right was preincubated with blocking peptide.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab131494 被引用在 2 文献中.
- Tang Y et al. Electroacupuncture Ameliorates Cognitive Impairment by Inhibiting the JNK Signaling Pathway in a Mouse Model of Alzheimer's Disease. Front Aging Neurosci 12:23 (2020). PubMed: 32116652
- Zhou Q et al. Anti-inflammation effects of the total saponin fraction from Dioscorea nipponica Makino on rats with gouty arthritis by influencing MAPK signalling pathway. BMC Complement Med Ther 20:261 (2020). PubMed: 32843018