Anti-MEK3 (phospho T222)抗体(ab79586)
Key features and details
- Rabbit polyclonal to MEK3 (phospho T222)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-MEK3 (phospho T222)抗体
参阅全部 MEK3 一抗 -
描述
兔多克隆抗体to MEK3 (phospho T222) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human MEK3 (phospho T222).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab79586 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab79586于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 39 kDa.
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| IHC-P |
1/50 - 1/100.
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| 说明 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 39 kDa. |
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IHC-P
1/50 - 1/100. |
靶标
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功能
Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. -
组织特异性
Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues. -
疾病相关
Note=Defects in MAP2K3 may be involved in colon cancer. -
序列相似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated.
Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity.
Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway. - Information by UniProt
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数据库链接
- Entrez Gene: 5606 Human
- Entrez Gene: 26397 Mouse
- Entrez Gene: 303200 Rat
- Omim: 602315 Human
- SwissProt: P46734 Human
- SwissProt: O09110 Mouse
- Unigene: 514012 Human
- Unigene: 18494 Mouse
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别名
- AW212142 antibody
- dual specificity mitogen activated protein kinase kinase 3 antibody
- Dual specificity mitogen-activated protein kinase kinase 3 antibody
see all
图片
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Western blot - Anti-MEK3 (phospho T222) antibody (ab79586)All lanes : Anti-MEK3 (phospho T222) antibody (ab79586) at 1/500 dilution
Lane 1 : Jurkat cell extracts treated
with serum (20 %, 15 minutes)
Lane 2 : Jurkat cell extracts treated
with serum (20 %, 15 minutes) with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 39 kDa
Observed band size: 39 kDa
Additional bands at: 32 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK3 (phospho T222) antibody (ab79586)ab79586, at a 1/50 dilution, staining MEK3 in paraffin embedded human brain tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunizing peptide.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab79586 被引用在 1 文献中.
- Ding J et al. The angiotensin receptor neprilysin inhibitor LCZ696 attenuates renal fibrosis via ASK1/JNK/p38 MAPK-mediated apoptosis in unilateral ureteral obstruction. PLoS One 18:e0286903 (2023). PubMed: 37310976