重组Anti-MEK3抗体[EP555Y] (ab40836)
![Western blot - Anti-MEK3 antibody [EP555Y] (ab40836)](https://www.abcam.cn/ps/products/40/ab40836/Images/ab40836-260986-ab40836KOWB.jpg "Western blot - Anti-MEK3 antibody [EP555Y] (ab40836)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP555Y] to MEK3
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-MEK3抗体[EP555Y]
参阅全部 MEK3 一抗 -
描述
兔单克隆抗体[EP555Y] to MEK3 -
宿主
Rabbit -
特异性
This antibody is specific for MEK3.
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经测试应用
适用于: WBmore details
不适用于: ICC/IF or IHC -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Jurkat, Hela, MCF-7 and K562 cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EP555Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab40836于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/5000 - 1/20000. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).
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| 说明 |
|---|
|
WB
1/5000 - 1/20000. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa). |
靶标
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功能
Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. -
组织特异性
Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues. -
疾病相关
Note=Defects in MAP2K3 may be involved in colon cancer. -
序列相似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated.
Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity.
Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway. - Information by UniProt
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数据库链接
- Entrez Gene: 5606 Human
- Omim: 602315 Human
- SwissProt: P46734 Human
- Unigene: 514012 Human
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别名
- AW212142 antibody
- dual specificity mitogen activated protein kinase kinase 3 antibody
- Dual specificity mitogen-activated protein kinase kinase 3 antibody
see all
图片
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Western blot - Anti-MEK3 antibody [EP555Y] (ab40836)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: MEK3knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab40836 observed at 150 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab40836 was shown to recognize MEK3 when MEK3 knockout samples were used, along with additional cross-reactive bands. Wild-type and MEK3 knockout samples were subjected to SDS-PAGE. ab40836 and ab8245 (loading control to GAPDH) were both diluted to 1/5000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-MEK3 antibody [EP555Y] (ab40836)](https://www.abcam.cn/ps/products/40/ab40836/Images/ab40836.jpg)
Western blot - Anti-MEK3 antibody [EP555Y] (ab40836)All lanes : Anti-MEK3 antibody [EP555Y] (ab40836) at 1/20000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Hela cell lysate
Lane 3 : MCF-7 cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 39 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted? -
![Anti-MEK3 antibody [EP555Y] (ab40836)](https://www.abcam.cn/ps/products/40/ab40836/Images/ab40836-3-benefits-of-recombinant-antibodies.png)
Anti-MEK3 antibody [EP555Y] (ab40836)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab40836 尚未被引用在任何文献中。