Anti-MEK1 (phospho T286)抗体(ab129431)
Key features and details
- Rabbit polyclonal to MEK1 (phospho T286)
- Suitable for: IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-MEK1 (phospho T286)抗体
参阅全部 MEK1 一抗 -
描述
兔多克隆抗体to MEK1 (phospho T286) -
宿主
Rabbit -
经测试应用
适用于: IP, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Cow, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese hamster, Elephant -
免疫原
Synthetic peptide corresponding to amino acids surrounding Threonine 286 of Human MEK1 when the Threonine residue is phosphorylated (NP_002746.1).
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阳性对照
- HeLa whole cell lysate, treated with nocodazole.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab129431 was affinity purified using an epitope specific to Phospho MEK1 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab129431于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use a concentration of 2 - 10 µg/ml.
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IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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IP
Use a concentration of 2 - 10 µg/ml. |
IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases. -
组织特异性
Widely expressed, with extremely low levels in brain. -
疾病相关
Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant. -
序列相似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity.
Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway. - Information by UniProt
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数据库链接
- Entrez Gene: 533199 Cow
- Entrez Gene: 5604 Human
- Entrez Gene: 26395 Mouse
- Entrez Gene: 100233191 Pig
- Entrez Gene: 170851 Rat
- Omim: 176872 Human
- SwissProt: Q63980 Chinese hamster
- SwissProt: Q02750 Human
see all -
别名
- Dual specificity mitogen activated protein kinase kinase 1 antibody
- Dual specificity mitogen-activated protein kinase kinase 1 antibody
- ERK activator kinase 1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 (phospho T286) antibody (ab129431)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal cell carcinoma tissue labelling MEK1 (phospho T286) with ab129431 at 1/1000 (1µg/ml). Detection: DAB.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 (phospho T286) antibody (ab129431)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MEK1 (phospho T286) with ab129431 at 1/5000 (0.2µg/ml). Detection: DAB.
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Detection of MEK1 by Western Blot of Immunprecipitate.
ab129431 at 1µg/ml (lanes 1-4) or Control IgG (lanes 5-6)staining MEK1 in HeLa whole cell lysate immunoprecipitated using ab129431 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 1: HeLa whole cell lysate, mock treated.
Lane 2: HeLa whole cell lysate, nocodazole treated.
Lane 3: Immunoprecipitation of MEK1 (phospho T286) from mock treated HeLa whole cell lysate.
Lane 4: Immunoprecipitation of MEK1 (phospho T286) from nocodazole treated HeLa whole cell lysate.
Lane 5: HeLa whole cell lysate, mock treated.
Lane 6: HeLa whole cell lysate, nocodazole treated.
Predicted band size : 43 kDa.
Detection: Chemiluminescence with exposure time 10 seconds.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab129431 尚未被引用在任何文献中。