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重组RabMAb

重组Anti-MEIS2抗体[EPR12100] (ab174270)

Reviews (1) Submit a question
Flow Cytometry (Intracellular) - Anti-MEIS2 antibody [EPR12100] (ab174270)
  • Western blot - Anti-MEIS2 antibody [EPR12100] (ab174270)
  • Anti-MEIS2 antibody [EPR12100] (ab174270)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12100] to MEIS2
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-MEIS2抗体[EPR12100]
    参阅全部 MEIS2 一抗

  • 描述

    兔单克隆抗体[EPR12100] to MEIS2

  • 宿主

    Rabbit

  • 特异性

    The immunogen of ab174270 is, according to BLAST results, highly conserved (93%) with human MEIS1. According to this, the antibody is very likely to cross-react with MEIS1 but this has, to our knowledge, not been experimentally tested so far.

  • 经测试应用

    适用于: Flow Cyt (Intra), WBmore details
    不适用于: ICC/IF,IHC-P or IP

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human MEIS2 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: O14770

  • 阳性对照

    • Fetal thymus, HeLa, HepG2 and K562 lysates. Permeabilized K562 cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab174270于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
说明
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
应用说明
Is unsuitable for ICC/IF,IHC-P or IP.

靶标

  • 组织特异性

    Expressed in various tissues. In hematopoietic tissues, the lymphoid organs express high levels of MEIS2. Also expressed in some regions of the brain, such as the putamen.

  • 序列相似性

    Belongs to the TALE/MEIS homeobox family.
    Contains 1 homeobox DNA-binding domain.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Homeobox protein Meis2 antibody
      • HsT18361 antibody
      • Meis (mouse) homolog 2 antibody
      • Meis homeobox 2 antibody
      • Meis homolog 2 antibody
      • Meis1 myeloid ecotropic viral integration site 1 homolog 2 (mouse) antibody
      • Meis1 myeloid ecotropic viral integration site 1 homolog 2 antibody
      • Meis1 related gene 1 antibody
      • Meis1 related protein 1 antibody
      • Meis1-related protein 1 antibody
      • MEIS2 antibody
      • MEIS2_HUMAN antibody
      • MGC2820 antibody
      • MRG1 antibody
      • TALE homeobox protein Meis2 antibody
      see all

    图片

    • Flow Cytometry (Intracellular) - Anti-MEIS2 antibody [EPR12100] (ab174270)
      Flow Cytometry (Intracellular) - Anti-MEIS2 antibody [EPR12100] (ab174270)

      Intracellular flow cytometric analysis of permeabilized K562 cells usingab174270 at a 1/10 dilution (red) or a rabbit IgG (negative) (green). 

       

       

    • Western blot - Anti-MEIS2 antibody [EPR12100] (ab174270)
      Western blot - Anti-MEIS2 antibody [EPR12100] (ab174270)
      All lanes : Anti-MEIS2 antibody [EPR12100] (ab174270) at 1/1000 dilution

      Lane 1 : Fetal thymus lysates
      Lane 2 : HeLa lysates
      Lane 3 : HepG2 lysates
      Lane 4 : K562 lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 52 kDa

    • Anti-MEIS2 antibody [EPR12100] (ab174270)
      Anti-MEIS2 antibody [EPR12100] (ab174270)

    文献 (0)

    发表研究结果有使用 ab174270？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab174270 尚未被引用在任何文献中。

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    Western blot abreview for Anti-MEIS2 antibody [EPR12100]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10 µg
    Treatment
    Overexpression
    Specification
    HEK293
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Sep 11 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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