Anti-MEF2D抗体(ab32845)
Key features and details
- Rabbit polyclonal to MEF2D
- Suitable for: IP, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-MEF2D抗体
参阅全部 MEF2D 一抗 -
描述
兔多克隆抗体to MEF2D -
宿主
Rabbit -
经测试应用
适用于: IP, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment, corresponding to amino acids 292-514 of Human MEF2D
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituent: Whole serum -
Concentration information loading...
-
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32845于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IP |
Use at an assay dependent concentration. Use 4µl for 1ml cell lysate.
|
|
IHC-P | (1) |
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
说明 |
---|
IP
Use at an assay dependent concentration. Use 4µl for 1ml cell lysate. |
IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
-
功能
Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific, growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. Plays a critical role in the regulation of neuronal apoptosis. -
序列相似性
Belongs to the MEF2 family.
Contains 1 MADS-box domain.
Contains 1 Mef2-type DNA-binding domain. -
发展阶段
Present in myotubes and also in undifferentiated myoblasts. -
结构域
The beta domain, missing in a number of isoforms, is required for enhancement of transcriptional activity. -
翻译后修饰
Phosphorylated on Ser-444 by CDK5 is required for Lys-439 sumoylation and inhibits transcriptional activity. In neurons, enhanced CDK5 activity induced by neurotoxins promotes caspase 3-mediated cleavage leading to neuron apoptosis. Phsophorylation on Ser-180 can be enhanced by EGF.
Acetylated on Lys-439 by CREBBP. Deacetylated by SIRT1.
Sumoylated on Lys-439 by SUMO2 but not SUMO1; which inhibits transcriptional activity and myogenic activity. Desumoylated by SENP3.
Proteolytically cleaved in cerebellar granule neurons on several sites by caspase 7 following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation. -
细胞定位
Nucleus. Translocated by HDAC4 to nuclear dots. - Information by UniProt
-
数据库链接
- Entrez Gene: 4209 Human
- Omim: 600663 Human
- SwissProt: Q14814 Human
- Unigene: 314327 Human
-
别名
- DKFZp686I1536 antibody
- MADS box transcription factor 2 polypeptide D antibody
- Mef2d antibody
see all
图片
-
Immunoprecipitation of 35S-methionine labelled HeLa cells with anti-MEF2D sera. 1: pre-immune sera; 2: ab32845 anti-MEF2D sera.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2D antibody (ab32845)ab32845 (2µg/ml) staining MEF2D in human skin using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
实验方案
数据表及文件
-
Datasheet download
文献 (15)
ab32845 被引用在 15 文献中.
- Pingul BY et al. Dissection of the MEF2D-IRF8 transcriptional circuit dependency in acute myeloid leukemia. iScience 25:105139 (2022). PubMed: 36193052
- Cilenti F et al. A PGE2-MEF2A axis enables context-dependent control of inflammatory gene expression. Immunity 54:1665-1682.e14 (2021). PubMed: 34129840
- Ling B et al. Nucleolar and spindle-associated protein 1 promotes non-small cell lung cancer progression and serves as an effector of myocyte enhancer factor 2D. Oncol Rep 45:1044-1058 (2021). PubMed: 33650655
- Huang L et al. Protocatechuic acid attenuates ß-secretase activity and okadaic acid-induced autophagy via the Akt/GSK-3ß/MEF2D pathway in PC12 cells. Mol Med Rep 21:1328-1335 (2020). PubMed: 31894327
- Cheng Y et al. NeuroD1 Dictates Tumor Cell Differentiation in Medulloblastoma. Cell Rep 31:107782 (2020). PubMed: 32579914