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重组RabMAb

重组Anti-MED4抗体[EPR7816] (ab129170)

Reviews (2) Submit a question References (4)
Western blot - Anti-MED4 antibody [EPR7816] (ab129170)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED4 antibody [EPR7816] (ab129170)
  • Immunocytochemistry/ Immunofluorescence - Anti-MED4 antibody [EPR7816] (ab129170)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED4 antibody [EPR7816] (ab129170)
  • OI-RD Scanning - Anti-MED4 antibody [EPR7816] (ab129170)
  • Anti-MED4 antibody [EPR7816] (ab129170)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7816] to MED4
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-MED4抗体[EPR7816]
    参阅全部 MED4 一抗

  • 描述

    兔单克隆抗体[EPR7816] to MED4

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-P, ICC/IFmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat, Xenopus laevis

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HepG2, T47D and K562 cell lysates. IHC-P: Human breast carcinoma and colon tissues. ICC/IF: HepG2 cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab129170于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (2)
1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 30 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
说明
WB
1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 30 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.

靶标

  • 功能

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

  • 序列相似性

    Belongs to the Mediator complex subunit 4 family.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Activator-recruited cofactor 36 kDa component antibody
    • ARC36 antibody
    • DRIP36 antibody
    • FLJ10956 antibody
    • HSPC126 antibody
    • med4 antibody
    • MED4_HUMAN antibody
    • Mediator complex subunit 4 antibody
    • Mediator of RNA polymerase II transcription subunit 4 antibody
    • Mediator of RNA polymerase II transcription, subunit 4 homolog antibody
    • OTTHUMP00000018390 antibody
    • OTTHUMP00000018391 antibody
    • OTTHUMP00000018392 antibody
    • RP11-90M2.2 antibody
    • TRAP/SMCC/PC2 subunit p36 subunit antibody
    • TRAP36 antibody
    • VDRIP antibody
    • Vitamin D3 receptor-interacting protein complex 36 kDa component antibody
    see all

图片

  • Western blot - Anti-MED4 antibody [EPR7816] (ab129170)
    Western blot - Anti-MED4 antibody [EPR7816] (ab129170)
    All lanes : Anti-MED4 antibody [EPR7816] (ab129170) at 1/1000 dilution

    Lane 1 : HepG2 cell lysates
    Lane 2 : T-47D cell lysates
    Lane 3 : K562 cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

    Predicted band size: 30 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED4 antibody [EPR7816] (ab129170)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED4 antibody [EPR7816] (ab129170)

    ab129170, at 1/100 dilution staining MED4 in paraffin-embedded Human breast carcinoma tissue, by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-MED4 antibody [EPR7816] (ab129170)
    Immunocytochemistry/ Immunofluorescence - Anti-MED4 antibody [EPR7816] (ab129170)

    Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling MED4 with ab129170 at 1/1000. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
    Control: PBS only.
    Nuclear counter stain: DAPI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED4 antibody [EPR7816] (ab129170)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED4 antibody [EPR7816] (ab129170)

    ab129170, at 1/100 dilution staining MED4 in paraffin-embedded Human colon tissue, by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-MED4 antibody [EPR7816] (ab129170)
    OI-RD Scanning - Anti-MED4 antibody [EPR7816] (ab129170)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-MED4 antibody [EPR7816] (ab129170)
    Anti-MED4 antibody [EPR7816] (ab129170)

文献 (4)

发表研究结果有使用 ab129170？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab129170 被引用在 4 文献中.

  • Pintacuda G  et al. Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders. Cell Genom 3:100250 (2023). PubMed: 36950384
  • Zhang N  et al. MED13L integrates Mediator-regulated epigenetic control into lung cancer radiosensitivity. Theranostics 10:9378-9394 (2020). PubMed: 32802198
  • Tuffy KM  et al. HIV-1 Gag Forms Ribonucleoprotein Complexes with Unspliced Viral RNA at Transcription Sites. Viruses 12:N/A (2020). PubMed: 33182496
  • Parrini M  et al. The C-Terminal Transactivation Domain of STAT1 Has a Gene-Specific Role in Transactivation and Cofactor Recruitment. Front Immunol 9:2879 (2018). PubMed: 30574148

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-MED4 antibody [EPR7816]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - nuclear (A549 cells)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
35 µg
Specification
A549 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 08 2016

Western blot abreview for Anti-MED4 antibody [EPR7816]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Xenopus laevis Cell lysate - other (Egg)
Loading amount
25 µg
Specification
Egg
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 27 2013

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