Anti-MED28/EG1抗体(ab73294)
Key features and details
- Rabbit polyclonal to MED28/EG1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-MED28/EG1抗体 -
描述
兔多克隆抗体to MED28/EG1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab73294于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
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| IHC-P |
Use a concentration of 2.5 µg/ml.
|
| 说明 |
|---|
|
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa). |
|
IHC-P
Use a concentration of 2.5 µg/ml. |
靶标
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功能
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways. -
组织特异性
Widely expressed. Highly expressed in vascular tissues such as placenta, testis and liver. -
序列相似性
Belongs to the Mediator complex subunit 28 family. -
细胞定位
Nucleus. Cytoplasm. Membrane. According to PubMed:15467741, it is cytoplasmic and mainly membrane-associated. - Information by UniProt
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数据库链接
- Entrez Gene: 80306 Human
- Omim: 610311 Human
- SwissProt: Q9H204 Human
- Unigene: 434075 Human
- Unigene: 644788 Human
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别名
- EG1 antibody
- Endothelial-derived protein 1 antibody
- FKSG20 antibody
see all
图片
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Western blot - Anti-MED28/EG1 antibody (ab73294)All lanes : Anti-MED28/EG1 antibody (ab73294) at 1 µg/ml
Lane 1 : human brain tissue lysate
Lane 2 : human brain tissue lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 20 kDa
Observed band size: 20 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED28/EG1 antibody (ab73294)ab73294 at 2.5µg/ml staining MED28/EG1 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab73294 尚未被引用在任何文献中。