AB73294

Anti-MED28/EG1抗体

Name: MED28/EG1抗体 (ab73294)| Abcam中文官网
Brand: Abcam Limited
SKU: AB73294
Price: 4606 CNY
Availability: InStock

Anti-MED28/EG1 antibody

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(1 Publication)

Rabbit Polyclonal MED28/EG1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.

查看别名

EG1, FKSG20, MED28, Mediator of RNA polymerase II transcription subunit 28, Endothelial-derived protein 1, Mediator complex subunit 28, Merlin and Grb2-interacting cytoskeletal protein, Tumor angiogenesis marker EG-1, Magicin

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED28/EG1 antibody (AB73294)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED28/EG1 antibody (AB73294)

ab73294 at 2.5μg/ml staining MED28/EG1 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

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Western blot - Anti-MED28/EG1 antibody (AB73294)

All lanes:

Western blot - Anti-MED28/EG1 antibody (ab73294) at 1 µg/mL

Lane 1:

human brain tissue lysate at 15 µg

Lane 2:

human brain tissue lysate at 15 µg with blocking peptide

Predicted band size: 20 kDa

Observed band size: 20 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2.5 µg/mL", "IHCP-species-notes": "<p></p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The protein MED28 also known as EG1 is an integral component of the mediator complex which plays an important role in regulating transcription. MED28 is a protein with a molecular mass of approximately 28 kDa. It is expressed in various tissues with notable presence in cardiac and skeletal muscle. The expression pattern suggests its involvement in complex cellular processes across different tissues.

Biological function summary

The MED28/EG1 protein serves as part of the larger mediator complex which connects transcription factors to RNA polymerase II during the transcription cycle. This complex acts as a transcriptional coactivator essential for the transcription of almost all class II gene promoters. MED28's contribution involves regulation of various genes hence it has a significant role in development and differentiation processes.

Pathways

MED28/EG1 is involved in essential pathways including the Wnt signaling pathway. It participates in modulating gene expression through interactions with other mediator complex proteins contributing to transcriptional regulation important for cellular responses and signaling networks. Other related proteins such as MED8 and MED12 alongside MED28 offer a cooperative function in mediator complex activities.

Aberrations in the function of MED28/EG1 associate with several conditions such as cancer and cardiovascular diseases. Dysregulation in its expression or mediator complex interactions could lead to inconsistencies in transcriptional activation contributing to the progression of these diseases. Studies have linked MED28 to proteins such as cyclin-dependent kinases which influence disease states by controlling cell cycle regulation and proliferation.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways.

See full target information MED28

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Regenerative therapy 21:87-95 PubMed35785044

2022

NUP160 knockdown inhibits the progression of diabetic nephropathy and .

Applications

Unspecified application

Species

Unspecified reactive species

Jiayong Xie,Zhi Chen,Gang Yao,Ying Yuan,Wenjuan Yu,Qiang Zhu

View all publications

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Anti-MED28/EG1抗体

Anti-MED28/EG1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED28/EG1 antibody (AB73294)

Western blot - Anti-MED28/EG1 antibody (AB73294)