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AB122368

Anti-MED12L抗体

Anti-MED12L antibody

4

(1 Review)

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(1 Publication)

Rabbit Polyclonal MED12L antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MED12L aa 600-750.

查看别名

KIAA1635, TNRC11L, TRALP, TRALPUSH, PRO0314, MED12L, Mediator of RNA polymerase II transcription subunit 12-like protein, Mediator complex subunit 12-like protein, Thyroid hormone receptor-associated-like protein, Trinucleotide repeat-containing gene 11 protein-like

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-MED12L antibody (AB122368)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MED12L antibody (AB122368)

Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleoli. Recommended concentration of ab122368 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED12L antibody (AB122368)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED12L antibody (AB122368)

ab122368, at 1/100 dilution, staining MED12L in paraffin-embedded Human liver tissue by immunohistochemistry.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

IHC-P, ICC/IF

applications

免疫原

Recombinant Fragment Protein within Human MED12L aa 600-750. The exact immunogen used to generate this antibody is proprietary information.

Q86YW9

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MED12L also known as Mediator of RNA polymerase II transcription subunit 12-like is a protein encoded by the MED12L gene in humans. It has a molecular weight of approximately 250 kDa. MED12L expression occurs widely throughout the body with a notable presence in tissues such as the brain liver and kidney. This protein plays a mechanical role in the transcriptional regulation interacting with multiple components during the regulatory process.
Biological function summary

The MED12L protein functions as part of the Mediator complex which is essential for the transcription of nearly all RNA polymerase II-dependent genes. The Mediator complex acts as a bridge between gene-specific regulatory proteins and the polymerase machinery facilitating gene expression. Through this complex MED12L contributes significantly to regulating developmental processes and cellular differentiation.

Pathways

MED12L is intimately involved in the Notch signaling pathway and the Wnt signaling pathway. These pathways are critical for cell fate determination proliferation and communication during embryonic development and in adult tissue homeostasis. MED12L interacts with other Mediator complex proteins such as MED1 and MED19 to facilitate these pathways and ensure proper signal transduction and transcriptional regulation.

MED12L has associations with intellectual disabilities and certain cancers. Mutations or dysregulation of MED12L can lead to disrupted Mediator complex function impacting cognitive development and potentially contributing to tumorigenesis. In cancer MED12L-related pathways may intersect with pathways involving proteins such as β-Catenin and Notch receptors therefore playing a role in oncogenic transformation and disease progression.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

May be a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).
See full target information MED12L
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Asian journal of andrology 27:245-253 PubMed39686771

2024

Novel homozygous SPAG17 variants cause human male infertility through multiple morphological abnormalities of spermatozoal flagella related to axonemal microtubule doublets.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Liu,Fazal Rahim,Meng-Lei Yang,Meftah Uddin,Jing-Wei Ye,Imtiaz Ali,Yousaf Raza,Abu Mansoor,Muhammad Shoaib,Mujahid Hussain,Ihsan Khan,Basit Shah,Asad Khan,Ahmad Nisar,Hui Ma,Bo Xu,Wasim Shah,Qing-Hua Shi
View all publications
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