Anti-ME1抗体(ab223761)
Key features and details
- Rabbit polyclonal to ME1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ME1抗体
参阅全部 ME1 一抗 -
描述
兔多克隆抗体to ME1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Pig
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免疫原
Recombinant fragment corresponding to Human ME1 aa 250-450.
Database link: P48163 -
阳性对照
- WB: Human plasma (IgG/HSA depleted); Human tonsil lysate, IHC-P: Human adrenal gland tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223761于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 64 kDa.
|
|
| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 64 kDa. |
|
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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组织特异性
Expressed in all tissues tested including liver, placenta and white adipose tissue. -
序列相似性
Belongs to the malic enzymes family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 4199 Human
- Entrez Gene: 17436 Mouse
- Entrez Gene: 397538 Pig
- Entrez Gene: 24552 Rat
- Omim: 154250 Human
- SwissProt: P48163 Human
- SwissProt: P06801 Mouse
- SwissProt: Q29558 Pig
see all -
别名
- Cytosolic malic enzyme 1 antibody
- HUMNDME antibody
- Malate dehydrogenase antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME1 antibody (ab223761)
Paraffin-embedded human adrenal gland tissue stained for ME1 using ab223761 at 1/50 dilution in immunohistochemical analysis.
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Western blot - Anti-ME1 antibody (ab223761)All lanes : Anti-ME1 antibody (ab223761) at 1/100 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) cell lysate
Lane 3 : Human plasma (IgG/HSA depleted)
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Predicted band size: 64 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab223761 尚未被引用在任何文献中。