Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-MDMX/MDM4 (phospho S367)抗体[15] (ab122926)

Submit a review Q&A (1)References (2)
Western blot - Anti-MDMX/MDM4 (phospho S367) antibody [15] (ab122926)

    Key features and details

    • Mouse monoclonal [15] to MDMX/MDM4 (phospho S367)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG2b

    概述

    • 产品名称

      Anti-MDMX/MDM4 (phospho S367)抗体[15]
      参阅全部 MDMX/MDM4 一抗

    • 描述

      小鼠单克隆抗体[15] to MDMX/MDM4 (phospho S367)

    • 宿主

      Mouse

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

    • 阳性对照

      • MCF cells reincubated with the proteasome inhibitor MG132 (20 uM) and exposed to DNA damaging agent, adriamycin (3 uM) or etoposide (20 uM).

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab122926于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    Use a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa.
    说明
    WB
    Use a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa.

    靶标

    • 功能

      Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions.

    • 组织特异性

      Expressed in all tissues tested with high levels in thymus.

    • 序列相似性

      Belongs to the MDM2/MDM4 family.
      Contains 1 RanBP2-type zinc finger.
      Contains 1 RING-type zinc finger.
      Contains 1 SWIB domain.

    • 结构域

      Region I is sufficient for binding TP53 and inhibiting its G1 arrest and apoptosis functions. It also binds TP73. Region II contains most of a central acidic region and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc mediates the heterooligomerization with MDM2.

    • 翻译后修饰

      Ubiquitinated. Deubiquitinated by USP2; leading to stabilize it.

    • 细胞定位

      Nucleus.
    • Information by UniProt

    • 数据库链接

      • 别名

        • DKFZp781B1423 antibody
        • Double minute 4 antibody
        • Double minute 4 human homolog of p53 binding protein antibody
        • Double minute 4 protein antibody
        • HDMX antibody
        • MDM 4 antibody
        • Mdm2 like p53 binding protein antibody
        • Mdm2-like p53-binding protein antibody
        • MDM4 antibody
        • Mdm4 p53 binding protein homolog mouse antibody
        • Mdm4 protein antibody
        • MDM4 related protein 1 antibody
        • Mdm4 transformed 3T3 cell double minute 4 antibody
        • Mdm4 transformed 3T3 cell double minute 4 p53 binding protein antibody
        • Mdm4 transformed 3T3 cell double minute 4 p53 binding protein mouse antibody
        • MDM4_HUMAN antibody
        • Mdmx protein antibody
        • MGC132766 antibody
        • Mouse double minute 4 homolog antibody
        • Mouse double minute 4 human homolog of p53 binding protein antibody
        • MRP 1 antibody
        • MRP1 antibody
        • p53 binding protein antibody
        • p53 BINDING PROTEIN MDM4 antibody
        • p53-binding protein Mdm4 antibody
        • Protein Mdm4 antibody
        • Protein Mdmx antibody
        see all

      图片

      • Western blot - Anti-MDMX/MDM4 (phospho S367) antibody [15] (ab122926)
        Western blot - Anti-MDMX/MDM4 (phospho S367) antibody [15] (ab122926)
        All lanes : Anti-MDMX/MDM4 (phospho S367) antibody [15] (ab122926) at 1 µg/ml

        Lane 1 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM)
        Lane 2 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to adriamycin (3 uM) for 1 hour
        Lane 3 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to adriamycin (3 uM) for 2 hour
        Lane 4 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to adriamycin (3 uM) for 3 hour
        Lane 5 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to adriamycin (3 uM) for 4 hour
        Lane 6 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to etoposide (20 uM) for 1 hour
        Lane 7 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to etoposide (20 uM) for 2 hour
        Lane 8 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to etoposide (20 uM) for 3 hour
        Lane 9 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to etoposide (20 uM) for 4 hour

        Predicted band size: 55 kDa



        The upper lanes show incubation with a non-phosphos antibody to MDMX/MDM4. The lower lanes as indicated above.
        The cell lysates were immunoprecipitated with another anti-MDMX/MDM4 antibody before western blotting.

      文献 (2)

      发表研究结果有使用 ab122926？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab122926 被引用在 2 文献中.

      • Zhang W  et al. PTPRO-mediated autophagy prevents hepatosteatosis and tumorigenesis. Oncotarget 6:9420-33 (2015). WB ; Mouse . PubMed: 25826083
      • Okamoto K  et al. DNA damage-induced phosphorylation of MdmX at serine 367 activates p53 by targeting MdmX for Mdm2-dependent degradation. Mol Cell Biol 25:9608-20 (2005). PubMed: 16227609

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      Question

      I look into your data sheet cannot find reference/pdfs for these antibodies:

      ab122926
      ab55334
      ab59425
      ab28820

      We areinterested inpublications for experiences example with those antibodies (WB, IHC, IF).

      Thank you very much for your help!

      Read More
      Answer

      Thank you for your inquiry.


      If we know of our antibodies being used in any references/publications, we will list these under the "Specific References" section of our datasheet, which can be found under the shipping and pricing information online.


      We know of one reference for ab112926: Okamoto K et al. DNA damage-induced phosphorylation of MdmX at serine 367 activates p53 by targeting MdmX for Mdm2-dependent degradation. Mol Cell Biol 25:9608-20 (2005). PubMed: 16227609


      https://www.abcam.com/MDMX-phospho-S367-antibody-15-ab122926-references.html


      We do not know of any publications for ab55334, ab59425, or ab28820. We would still guarantee these antibodies to work as stated on the datasheets in accordance with our Abpromise.


      I hope this information helps. Please contact us with any other questions.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com