Anti-MDM2 (phospho S166)抗体(ab131355)
Key features and details
- Rabbit polyclonal to MDM2 (phospho S166)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-MDM2 (phospho S166)抗体
参阅全部 MDM2 一抗 -
描述
兔多克隆抗体to MDM2 (phospho S166) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Synthetic peptide corresponding to Human MDM2 conjugated to keyhole limpet haemocyanin. Synthetic phosphopeptide conjugated to KLH, surrounding phosphorylation site of Serine 166 (
A-I-S(p)-E-T
) derived from Human MDM2 (NP_002383.2).
Database link: Q00987-1 -
阳性对照
- 293 cell extract treated with Hydroxyurea, HeLa cells, Human breast carcinoma tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab131355 is purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131355于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 55 kDa).
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|
IHC-P |
1/50 - 1/100.
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ICC/IF |
1/100 - 1/200.
|
说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 55 kDa). |
IHC-P
1/50 - 1/100. |
ICC/IF
1/100 - 1/200. |
靶标
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功能
E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as an ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. -
组织特异性
Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues. -
疾病相关
Note=Seems to be amplified in certain tumors (including soft tissue sarcomas, osteosarcomas and gliomas). A higher frequency of splice variants lacking p53 binding domain sequences was found in late-stage and high-grade ovarian and bladder carcinomas. Four of the splice variants show loss of p53 binding. -
序列相似性
Belongs to the MDM2/MDM4 family.
Contains 1 RanBP2-type zinc finger.
Contains 1 RING-type zinc finger.
Contains 1 SWIB domain. -
结构域
Region I is sufficient for binding p53 and inhibiting its G1 arrest and apoptosis functions. It also binds p73 and E2F1. Region II contains most of a central acidic region required for interaction with ribosomal protein L5 and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc interacts specifically with RNA whether or not zinc is present and mediates the heterooligomerization with MDM4. It is also essential for its ubiquitin ligase E3 activity toward p53 and itself. -
翻译后修饰
Phosphorylated in response to ionizing radiation in an ATM-dependent manner.
Auto-ubiquitinated; which leads to proteasomal degradation. Deubiquitinated by USP2 leads to its accumulation and increases deubiquitinilation and degradation of p53/TP53. Deubiquitinated by USP7; leading to stabilize it. -
细胞定位
Nucleus > nucleoplasm. Cytoplasm. Nucleus > nucleolus. Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins. Colocalizes with RASSF1 isoform A in the nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 4193 Human
- Entrez Gene: 17246 Mouse
- Omim: 164785 Human
- SwissProt: Q00987 Human
- SwissProt: P23804 Mouse
- Unigene: 484551 Human
- Unigene: 22670 Mouse
- Unigene: 447669 Mouse
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别名
- ACTFS antibody
- Double minute 2 protein antibody
- E3 ubiquitin-protein ligase Mdm2 antibody
see all
图片
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All lanes : Anti-MDM2 (phospho S166) antibody (ab131355) at 1/500 dilution
Lane 1 : 293 cell extract untreated
Lane 2 : 293 cell extract treated with hydroxyurea
Predicted band size: 55 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDM2 (phospho S166) antibody (ab131355)Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling MDM2 (phospho S166) with ab131355 at a dilution of 1/50. Picture on the right was preincubated with blocking peptide.
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Immunofluorescence of methanol-fixed HeLa cells staining MDM2 (phospho S166) with ab131355 at a dilution of 1/100.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab131355 被引用在 5 文献中.
- Barrio E et al. Preconditioning-Activated AKT Controls Neuronal Tolerance to Ischemia through the MDM2-p53 Pathway. Int J Mol Sci 22:N/A (2021). PubMed: 34298892
- Fukui Y et al. Investigation of the molecular causes underlying physical abnormalities in Diamond-Blackfan anemia patients with RPL5 haploinsufficiency. Pathol Int 71:803-813 (2021). PubMed: 34587661
- Rehman A et al. The desmosomal cadherin desmoglein-3 acts as a keratinocyte anti-stress protein via suppression of p53. Cell Death Dis 10:750 (2019). PubMed: 31582719
- Wu DM et al. MircoRNA-1275 promotes proliferation, invasion and migration of glioma cells via SERPINE1. J Cell Mol Med 22:4963-4974 (2018). PubMed: 30024092
- Lim EG et al. Ethanol extract from Cnidium monnieri (L.) Cusson induces cell cycle arrest and apoptosis via regulation of the p53-independent pathway in HepG2 and Hep3B hepatocellular carcinoma cells. Mol Med Rep 17:2572-2580 (2018). PubMed: 29207130