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Anti-MDH2抗体[2F5AF8] (ab110317)

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Immunocytochemistry/ Immunofluorescence - Anti-MDH2 antibody [2F5AF8] (ab110317)
  • Immunoprecipitation - Anti-MDH2 antibody [2F5AF8] (ab110317)

Key features and details

  • Mouse monoclonal [2F5AF8] to MDH2
  • Suitable for: ICC/IF, IP
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-MDH2抗体[2F5AF8]
    参阅全部 MDH2 一抗

  • 描述

    小鼠单克隆抗体[2F5AF8] to MDH2

  • 宿主

    Mouse

  • 经测试应用

    适用于: ICC/IF, IPmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Full length protein. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • HDFn fibroblast cells Human heart homogenate Rat liver homogenate Mouse liver homogenate HepG2 whole cell lysate (ab7900)

  • 常规说明

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab110317于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Use a concentration of 1 - 4 µg/ml.
IP
Use at an assay dependent concentration.
说明
ICC/IF
Use a concentration of 1 - 4 µg/ml.
IP
Use at an assay dependent concentration.

靶标

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-MDH2 antibody [2F5AF8] (ab110317)
    Immunocytochemistry/ Immunofluorescence - Anti-MDH2 antibody [2F5AF8] (ab110317)

    Immunocytochemistry image of stained HDFn (Human dermal fibroblasts) fibroblast cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with the ab110317 (1 µg/mL) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondrial matrix.

  • Immunoprecipitation - Anti-MDH2 antibody [2F5AF8] (ab110317)
    Immunoprecipitation - Anti-MDH2 antibody [2F5AF8] (ab110317)
    ab110317 pulls down the 33kDa MDH2 protein from Human heart homogenate (1), Rat liver homogenate (2), Mouse liver homogenate (3) and HepG2 whole cell lysate (4). The identity of this protein was confirmed by mass spectrometry. This gel was stained with coomassie.

    Lane 1 : Anti-MDH2 antibody [2F5AF8] (ab110317)


    All lanes :

文献 (3)

发表研究结果有使用 ab110317？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab110317 被引用在 3 文献中.

  • Li Y  et al. Intermittent fasting promotes adipocyte mitochondrial fusion through Sirt3-mediated deacetylation of Mdh2. Br J Nutr 130:1473-1486 (2023). PubMed: 36815302
  • Ou T  et al. SIRT5 deficiency enhances the proliferative and therapeutic capacities of adipose-derived mesenchymal stem cells via metabolic switching. Clin Transl Med 10:e172 (2020). PubMed: 32997407
  • Ma X  et al. Dietary L-arginine supplementation affects the skeletal longissimus muscle proteome in finishing pigs. PLoS One 10:e0117294 (2015). WB ; Pig . PubMed: 25635834

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