Anti-MDH1抗体(ab175455)
Key features and details
- Goat polyclonal to MDH1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
概述
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产品名称
Anti-MDH1抗体
参阅全部 MDH1 一抗 -
描述
山羊多克隆抗体to MDH1 -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human, Pig
预测可用于: Chicken, Cow, Cat, Dog, Xenopus laevis, Xenopus tropicalis
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免疫原
Synthetic peptide corresponding to Human MDH1 aa 135-149 (internal sequence). NP_001186040.1; NP_005908.1; NP_001186041.1.
Sequence:TNCLTASKSAPSIPK
Database link: P40925 -
阳性对照
- WB: Human, mouse, pig and rat heart lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab175455于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
|
WB
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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序列相似性
Belongs to the LDH/MDH superfamily. MDH type 2 family. -
翻译后修饰
ISGylated. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 493924 Cat
- Entrez Gene: 535182 Cow
- Entrez Gene: 4190 Human
- Entrez Gene: 17449 Mouse
- Entrez Gene: 396894 Pig
- Entrez Gene: 24551 Rat
- Entrez Gene: 398872 Xenopus laevis
- Omim: 154200 Human
see all -
别名
- cytoplasmic antibody
- Cytosolic malate dehydrogenase antibody
- Diiodophenylpyruvate reductase antibody
see all
图片
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Western blot - Anti-MDH1 antibody (ab175455)All lanes : Anti-MDH1 antibody (ab175455) at 0.01 µg/ml
Lane 1 : Human heart lysate (in RIPA buffer)
Lane 2 : Mouse heart lysate (in RIPA buffer)
Lane 3 : Rat heart lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 36 kDa
Observed band size: 36 kDaPrimary incubation was 1 hour.
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Western blot - Anti-MDH1 antibody (ab175455)Anti-MDH1 antibody (ab175455) at 0.01 µg/ml + Pig heart lysate ( in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 36 kDa
Observed band size: 36 kDaPrimary incubation was 1 hour.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab175455 被引用在 1 文献中.
- Li B et al. Overexpression of PTEN may increase the effect of pemetrexed on A549 cells via inhibition of the PI3K/AKT/mTOR pathway and carbohydrate metabolism. Mol Med Rep 20:3793-3801 (2019). PubMed: 31485626