重组Anti-MDA5抗体[EPR6743] (ab126630)
![Western blot - Anti-MDA5 antibody [EPR6743] (ab126630)](https://www.abcam.cn/ps/products/126/ab126630/Images/ab126630-517143-anti-mda5-antibody-epr6743-western-blot-wildtype-jurkat-ifih1-knockout-jurkat.jpg "Western blot - Anti-MDA5 antibody [EPR6743] (ab126630)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6743] to MDA5
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-MDA5抗体[EPR6743]
参阅全部 MDA5 一抗 -
描述
兔单克隆抗体[EPR6743] to MDA5 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- THP1 treated with LPS cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6743 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab126630于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 117 kDa.
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| 说明 |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 117 kDa. |
应用说明
Is unsuitable for Flow Cyt,IHC-P or IP.
靶标
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功能
RNA helicase that, through its ATP-dependent unwinding of RNA, may function to promote message degradation by specific RNases. Seems to have growth suppressive properties. Involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). ATPase activity is specifically induced by dsRNA. Essential for the production of interferons in response to picornaviruses. -
组织特异性
Widely expressed, at a low level. Expression is detected at slightly highest levels in placenta, pancreas and spleen and at barely levels in detectable brain, testis and lung. -
疾病相关
Genetic variation in IFIH1 is associated with diabetes mellitus insulin-dependent type 19 (IDDM19) [MIM:610155]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
Note=IFIH1 is the CADM-140 autoantigen, involved in clinically amyopathic dermatomyositis (CADM). This is a chronic inflammatory disorder that shows typical skin manifestations of dermatomyositis but has no or little evidence of clinical myositis. Anti-CADM-140 antibodies appear to be specific to dermatomyositis, especially CADM. Patients with anti-CADM-140 antibodies frequently develop life-threatening acute progressive interstitial lung disease (ILD). -
序列相似性
Belongs to the helicase family.
Contains 2 CARD domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
翻译后修饰
During apoptosis, processed into 3 cleavage products. The helicase-containing fragment, once liberated from the CARD domains, translocate from the cytoplasm to the nucleus. The processed protein significantly sensitizes cells to DNA degradation. -
细胞定位
Cytoplasm. Nucleus. May be found in the nucleus, during apoptosis. - Information by UniProt
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数据库链接
- Entrez Gene: 64135 Human
- Omim: 606951 Human
- SwissProt: Q9BYX4 Human
- Unigene: 163173 Human
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别名
- CADM-140 autoantigen antibody
- Clinically amyopathic dermatomyositis autoantigen 140 kDa antibody
- DEAD/H (Asp Glu Ala Asp/His) box polypeptide antibody
see all
图片
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Western blot - Anti-MDA5 antibody [EPR6743] (ab126630)All lanes : Anti-MDA5 antibody [EPR6743] (ab126630) at 1/1000 dilution
Lane 1 : Wild-type Jurkat, LPS (1 ug/mL, 24 h) cell lysate
Lane 2 : Wild-type Jurkat, vehicle control LPS (0 ug/mL, 24 h) cell lysate
Lane 3 : IFIH1 knockout Jurkat, LPS (1 ug/mL, 24 h) cell lysate
Lane 4 : IFIH1 knockout Jurkat, vehicle control LPS (0 ug/mL, 24 h) cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 117 kDa
Observed band size: 140, 90 kDa why is the actual band size different from the predicted?Western blot: Anti-IFIH1 antibody [EPR6743] (ab126630) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab126630 was shown to bind specifically to IFIH1. A band was observed at 140, 90 kDa in wild-type Jurkat cell lysates with no signal observed at this size in IFIH1 knockout cell line. To generate this image, wild-type and IFIH1 knockout Jurkat cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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![Western blot - Anti-MDA5 antibody [EPR6743] (ab126630)](https://www.abcam.cn/ps/products/126/ab126630/Images/ab126630-140636-anti-mda5-antibody-epr6743-western-blot.jpg)
Western blot - Anti-MDA5 antibody [EPR6743] (ab126630)Anti-MDA5 antibody [EPR6743] (ab126630) at 1/1000 dilution + THP1 treated with LPS cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 117 kDa -
![Anti-MDA5 antibody [EPR6743] (ab126630)](https://www.abcam.cn/ps/products/126/ab126630/Images/ab126630-3-benefits-of-recombinant-antibodies.png)
Anti-MDA5 antibody [EPR6743] (ab126630)
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab126630 被引用在 8 文献中.
- Lopacinski AB et al. Modeling the complete kinetics of coxsackievirus B3 reveals human determinants of host-cell feedback. Cell Syst 12:304-323.e13 (2021). PubMed: 33740397
- Werner A et al. Widespread formation of double-stranded RNAs in testis. Genome Res 31:1174-86 (2021). PubMed: 34158368
- Xu W et al. Long non-coding RNA GAS5 accelerates oxidative stress in melanoma cells by rescuing EZH2-mediated CDKN1C downregulation. Cancer Cell Int 20:116 (2020). PubMed: 32308561
- Zhao X et al. Activation of C-Type Lectin Receptor and (RIG)-I-Like Receptors Contributes to Proinflammatory Response in Middle East Respiratory Syndrome Coronavirus-Infected Macrophages. J Infect Dis 221:647-659 (2020). PubMed: 31562757
- Zhuang T et al. Intracellular virus sensor MDA5 exacerbates vitiligo by inducing the secretion of chemokines in keratinocytes under virus invasion. Cell Death Dis 11:453 (2020). PubMed: 32532953
- Mateer EJ et al. Visualization of Double-Stranded RNA Colocalizing With Pattern Recognition Receptors in Arenavirus Infected Cells. Front Cell Infect Microbiol 8:251 (2018). PubMed: 30087859
- Yi L et al. Interferon regulatory factor 3 mediates Poly(I:C)-induced innate immune response and apoptosis in non-small cell lung cancer. Int J Oncol 52:1623-1632 (2018). PubMed: 29512705
- Zhang Y et al. Hantaan virus infection induces CXCL10 expression through TLR3, RIG-I, and MDA-5 pathways correlated with the disease severity. Mediators Inflamm 2014:697837 (2014). WB ; Human . PubMed: 24701034