Anti-MD2抗体(ab24182)
Key features and details
- Rabbit polyclonal to MD2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-MD2抗体 -
描述
兔多克隆抗体to MD2 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
Via peptide column. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab24182于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (5) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 18 kDa).
|
| ICC/IF | (2) |
Use at an assay dependent concentration.
|
| IHC-P | (5) |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 18 kDa). |
|
ICC/IF
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Cooperates with TLR4 in the innate immune response to bacterial lipopolysaccharide (LPS), and with TLR2 in the response to cell wall components from Gram-positive and Gram-negative bacteria. Enhances TLR4-dependent activation of NF-kappa-B. Cells expressing both MD2 and TLR4, but not TLR4 alone, respond to LPS. -
翻译后修饰
N-glycosylated; high-mannose. -
细胞定位
Secreted > extracellular space. - Information by UniProt
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数据库链接
- Entrez Gene: 23643 Human
- Entrez Gene: 17087 Mouse
- Entrez Gene: 448830 Rat
- Omim: 605243 Human
- SwissProt: Q9Y6Y9 Human
- SwissProt: Q9JHF9 Mouse
- Unigene: 726603 Human
- Unigene: 116844 Mouse
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别名
- ESOP 1 antibody
- ESOP-1 antibody
- ESOP1 antibody
see all
图片
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Western blot - Anti-MD2 antibody (ab24182)This image is courtesy of an Abreview submitted by C. GuerriAll lanes : Anti-MD2 antibody (ab24182) at 2 µg/ml (in 5% milk in TBS/T 0.1% (blocking buffer) for 12 hours at 4°C)
Lane 1 : Whole cell lysate of rat cortical astrocytes treated with control siRNA for 96 hours
Lane 2 : Whole cell lysate of rat cortical astrocytes treated with control siRNA for 96 hours and LPS (50 ng/ml) for 10 minutes
Lane 3 : Whole cell lysate of rat cortical astrocytes treated with control siRNA for 96 hours and ethanol (50 mM) for 10 minutes
Lane 4 : Whole cell lysate of rat cortical astrocytes treated with MD2 siRNA for 96 hours
Lane 5 : Whole cell lysate of rat cortical astrocytes treated with MD2 siRNA for 96 hours and LPS (50 ng/ml) for 10 minutes
Lane 6 : Whole cell lysate of rat cortical astrocytes treated with MD2 siRNA for 96 hours and ethanol (50 mM) for 10 minutes
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : An HRP-conjugated Goat anti-rabbit IgG at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
Blocking Step: 5% Milk for 1 hour at room temperature. -
Western blot - Anti-MD2 antibody (ab24182)Anti-MD2 antibody (ab24182) at 1 µg/ml + Daudi cell lysate at 15 µg
Secondary
Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 18 kDa -
Western blot - Anti-MD2 antibody (ab24182) -
Western blot - Anti-MD2 antibody (ab24182)All lanes : Anti-MD2 antibody (ab24182) at 1 µg/ml
Lane 1 : Mouse spleen lysate
Lane 2 : Mouse thymus lysate
Lane 3 : Mouse testis lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 18 kDa -
Western blot - Anti-MD2 antibody (ab24182)All lanes : Anti-MD2 antibody (ab24182)
All lanes : Rat spleen lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 18 kDa -
Western blot - Anti-MD2 antibody (ab24182)Anti-MD2 antibody (ab24182) at 1 µg/ml (mouse spleen lysate)
Predicted band size: 18 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MD2 antibody (ab24182)ab24182 at 2µg/ml staining of MD2 in rat spleen cells by IHC
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Immunocytochemistry/ Immunofluorescence - Anti-MD2 antibody (ab24182)Immunofluorescent analysis of MD2 in Rat Spleen cells with ab24182 at 10 μg/mL in ICC/IF.
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Immunocytochemistry/ Immunofluorescence - Anti-MD2 antibody (ab24182)Immunofluorescent analysis of MD2 in human spleen cells with ab24182 at 20 μg/mL in ICC/IF. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MD2 antibody (ab24182)Immunohistochemical analysis of human spleen tissue stained for MD2 using ab24182 at 2.5μg/mL.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (43)
ab24182 被引用在 43 文献中.
- Chen S et al. Rutin alleviates ventilator-induced lung injury by inhibiting NLRP3 inflammasome activation. iScience 26:107866 (2023). PubMed: 37817937
- Fang Q et al. Caffeic acid phenethyl ester suppresses metastasis of breast cancer cells by inactivating FGFR1 via MD2. PLoS One 18:e0289031 (2023). PubMed: 37490511
- Xiaofeng G et al. PERK-STING-RIPK3 pathway facilitates cognitive impairment by inducing neuronal necroptosis in sepsis-associated encephalopathy. CNS Neurosci Ther 29:1178-1191 (2023). PubMed: 36694328
- Lin K et al. Direct cardio-protection of Dapagliflozin against obesity-related cardiomyopathy via NHE1/MAPK signaling. Acta Pharmacol Sin 43:2624-2635 (2022). PubMed: 35217813
- Chen S et al. IL35 attenuated LPS-induced acute lung injury by regulating macrophage polarization. Mol Biol Rep 49:5811-5820 (2022). PubMed: 35748972