AB129290

Anti-MCT2抗体

Anti-MCT2 antibody

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(4 Publications)

Goat Polyclonal MCT2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human SLC16A7 aa 450 to C-terminus.

查看别名

MCT2, SLC16A7, Monocarboxylate transporter 2, MCT 2, Solute carrier family 16 member 7

2 Images

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Western blot - Anti-MCT2 antibody (AB129290)

All lanes:

Western blot - Anti-MCT2 antibody (ab129290) at 1 µg/mL

All lanes:

A549 lysate in RIPA buffer at 35 µg

Predicted band size: 52 kDa

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCT2 antibody (AB129290)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCT2 antibody (AB129290)

ab129290, at 3.75μg/ml, staining MCT2 in Formalin-fixed, Paraffin-embedded Human testis tissue by Immunohistochemistry.

关键信息

宿主种属

Goat

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IHC-P

applications

免疫原

Synthetic Peptide within Human SLC16A7 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O60669

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2.5-3.75 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Common marmoset": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MCT2 also known as monocarboxylate transporter 2 or SLC16A7 plays a mechanical role in the transport of monocarboxylates such as lactate pyruvate and ketone bodies across cell membranes. With a molecular mass of approximately 53 kDa MCT2 is expressed in various tissues including the brain liver and skeletal muscles. It is in the plasma membrane where it facilitates the rapid exchange of substrates important for energy metabolism in cells.

Biological function summary

MCT2 facilitates the movement of lactate and other monocarboxylates impacting cellular energy production and homeostasis. It may associate with chaperone proteins like basigin or CD147 which assist in its proper localization in the membrane. This transporter plays a role in metabolic communication between cells reflecting its importance in maintaining high-energy demands especially in neurons and muscle cells.

Pathways

MCT2 has a vital function in glycolysis and oxidative phosphorylation by mediating lactate transport. It participates in the Cori cycle which integrates with glucose metabolism and works alongside lactate dehydrogenase (LDH) to shuttle lactate for gluconeogenesis in the liver. MCT2's operation affects cellular energy balance and tissue responses to metabolic fluctuations.

MCT2 has connections to cancer and neurological disorders such as epilepsy. In cancer altered MCT2 expression affects tumor metabolism and progression interacting with hypoxia-inducible factor-1 (HIF-1) which controls glycolytic enzymes. In neurological conditions deficient or excessive lactate transport by MCT2 could impact neuronal excitability and energy balance potentially linking it to disease-related proteins like glutamate receptors.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Proton-coupled monocarboxylate symporter. Catalyzes the rapid transport across the plasma membrane of monocarboxylates such as L-lactate, pyruvate and ketone bodies, acetoacetate, beta-hydroxybutyrate and acetate (PubMed : 32415067, PubMed : 9786900). Dimerization is functionally required and both subunits work cooperatively in transporting substrate (PubMed : 32415067).

See full target information SLC16A7

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Medical oncology (Northwood, London, England) 36:76 PubMed31342270

2019

Programmed death-1 receptor (PD-1) and PD-ligand-1 (PD-L1) expression in non-small cell lung cancer and the immune-suppressive effect of anaerobic glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra Giatromanolaki,Ioannis M Koukourakis,Konstantina Balaska,Achilleas G Mitrakas,Adrian L Harris,Michael I Koukourakis

Scientific reports 8:9814 PubMed29959350

2018

Utilization of lactic acid in human myotubes and interplay with glucose and fatty acid metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Jenny Lund,Vigdis Aas,Ragna H Tingstad,Alfons Van Hees,Nataša Nikolić

Cancer biology & medicine 14:396-404 PubMed29372106

2018

Thermogenic protein UCP1 and UCP3 expression in non-small cell lung cancer: relation with glycolysis and anaerobic metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra Giatromanolaki,Konstantina Balaska,Dimitra Kalamida,Christos Kakouratos,Efthimios Sivridis,Michael I Koukourakis

PloS one 9:e105038 PubMed25225794

2014

Monocarboxylate transporters 1-4 in NSCLC: MCT1 is an independent prognostic marker for survival.

Applications

IHC-P

Species

Human

Marte Eilertsen,Sigve Andersen,Samer Al-Saad,Yury Kiselev,Tom Donnem,Helge Stenvold,Ingvild Pettersen,Khalid Al-Shibli,Elin Richardsen,Lill-Tove Busund,Roy M Bremnes

View all publications

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Anti-MCT2抗体

Anti-MCT2 antibody

Western blot - Anti-MCT2 antibody (AB129290)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCT2 antibody (AB129290)