Anti-MCPH1抗体(ab121277)
Key features and details
- Rabbit polyclonal to MCPH1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-MCPH1抗体 -
描述
兔多克隆抗体to MCPH1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human MCPH1 aa 555-701.
Database link: Q8NEM0 -
阳性对照
- IHC-P: Human cerebral cortex, cerebellum and testis tissue sections. WB: NTERA-2 cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121277于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
|
| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
|
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Implicated in chromosome condensation and DNA damage induced cellular responses. May play a role in neurogenesis and regulation of the size of the cerebral cortex. -
组织特异性
Expressed in fetal brain, liver and kidney. -
疾病相关
Defects in MCPH1 are the cause of microcephaly primary type 1 (MCPH1) [MIM:251200]; also known as true microcephaly or microcephaly vera. Microcephaly is defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits. This entity is inherited as autosomal recessive trait. -
序列相似性
Contains 3 BRCT domains. -
细胞定位
Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
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数据库链接
- Entrez Gene: 79648 Human
- Omim: 607117 Human
- SwissProt: Q8NEM0 Human
- Unigene: 656769 Human
- Unigene: 708770 Human
- Unigene: 721952 Human
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别名
- BRCT repeat inhibitor of TERT expression 1 antibody
- BRIT 1 antibody
- FLJ12847 antibody
see all
图片
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Western blot - Anti-MCPH1 antibody (ab121277)Anti-MCPH1 antibody (ab121277) at 0.4 µg/ml + NTERA-2 (human malignant pluripotent embryonic carcinoma cell line) cell lysate
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCPH1 antibody (ab121277)Immunohistochemical analysis of paraffin embedded human testis tissue labeling MCPH1 with ab121277 antibody at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCPH1 antibody (ab121277)Immunohistochemical analysis of paraffin embedded human cerebral cortex tissue labeling MCPH1 with ab121277 antibody at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCPH1 antibody (ab121277)Immunohistochemical analysis of paraffin embedded human cerebellum tissue labeling MCPH1 with ab121277 antibody at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCPH1 antibody (ab121277)Immunohistochemical analysis of paraffin embedded human liver tissue labeling MCPH1 with ab121277 antibody at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab121277 被引用在 3 文献中.
- Liu L et al. Silencing BRIT1 Facilitates the Abilities of Invasiveness and Migration in Trophoblast Cells. Med Sci Monit 24:7451-7458 (2018). PubMed: 30337515
- Ge C et al. The UBC Domain Is Required for BRUCE to Promote BRIT1/MCPH1 Function in DSB Signaling and Repair Post Formation of BRUCE-USP8-BRIT1 Complex. PLoS One 10:e0144957 (2015). PubMed: 26683461
- Ge C et al. BRUCE regulates DNA double-strand break response by promoting USP8 deubiquitination of BRIT1. Proc Natl Acad Sci U S A 112:E1210-9 (2015). PubMed: 25733871