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AB245083

重组Anti-MCP4抗体[EPR19877-83] - BSA and Azide free (Detector)

Anti-MCP4 antibody [EPR19877-83] - BSA and Azide free (Detector)

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Anti-MCP4 antibody [EPR19877-83] - BSA and Azide free (Detector) (ab245083) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting MCP4 in ELISA. Suitable for Human, Monkey.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency

查看别名

MCP4, NCC1, SCYA13, CCL13, C-C motif chemokine 13, CK-beta-10, Monocyte chemoattractant protein 4, Monocyte chemotactic protein 4, NCC-1, Small-inducible cytokine A13, MCP-4

2 Images
Sandwich ELISA - Anti-MCP4 antibody [EPR19877-83] - BSA and Azide free (Detector) (AB245083)
  • sELISA

Unknown

Sandwich ELISA - Anti-MCP4 antibody [EPR19877-83] - BSA and Azide free (Detector) (AB245083)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab239426).

Sandwich ELISA - Anti-MCP4 antibody [EPR19877-83] - BSA and Azide free (Detector) (AB245083)
  • sELISA

Lab

Sandwich ELISA - Anti-MCP4 antibody [EPR19877-83] - BSA and Azide free (Detector) (AB245083)

Sandwich ELISA of ab244169 with the capture antibody ab244810 dilution at 2 μg/mL and detector antibody ab245083 dilution at 0.5 μg/mL.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR19877-83

亚型

IgG

不含载体蛋白

Yes

反应种属

Human, Monkey

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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搭配使用产品

Assay Kits

AB239426

Human MCP4 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human MCP4 ELISA Kit (AB239426)

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Sample Prep & Detection Kits

AB102899

PE/Cy5.5® Conjugation Kit - Lightning-Link®

Flow Cytometry - PE/Cy5.5® Conjugation Kit - Lightning-Link® (AB102899)

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Sample Prep & Detection Kits

AB188215

Gold Conjugation Kit (20 nm, 20 OD)

Conjugation - Gold Conjugation Kit (20 nm, 20 OD) (AB188215)

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Primary Antibodies

AB252575

Anti-GM-CSF antibody [EPR22272-113] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-GM-CSF antibody [EPR22272-113] - BSA and Azide free (Capture) (AB252575)

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Primary Antibodies

AB242994

Anti-IL-22 antibody [EPR19944-215] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-IL-22 antibody [EPR19944-215] - BSA and Azide free (Detector) (AB242994)

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Primary Antibodies

AB244964

Anti-LTA antibody [EPR20507-260] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-LTA antibody [EPR20507-260] - BSA and Azide free (Detector) (AB244964)

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Primary Antibodies

AB242515

Anti-IL-8 antibody [EPR19358-37] - BSA and Azide free (Capture)

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-8 antibody [EPR19358-37] - BSA and Azide free (Capture) (AB242515)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Monkey": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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产品详情

ab245083 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human/Monkey MCP4. The recommended pair for sandwich ELISA is:
Capture: ab244810, Human/Monkey MCP4 Capture Antibody (unconjugated)
Detector: ab245083, Human/Monkey MCP4 Detector Antibody (unconjugated)
The reference range value is 3.9 - 250 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MCP4 also known as CCL13 is a chemokine with a molecular mass of approximately 11 kDa. It plays a critical role in attracting immune cells to sites of inflammation. MCP4 achieves this through its interaction with G protein-coupled receptors particularly CCR2 and CCR3. The protein is primarily expressed in tissues involved in immune responses including lymph nodes spleen and bone marrow. It exhibits high expression levels at sites of allergic inflammation suggesting a specific role in these processes.
Biological function summary

MCP4 functions by mediating the recruitment of monocytes eosinophils and T lymphocytes. It is not part of a larger protein complex but rather operates as part of the chemokine signaling family. MCP4 is particularly active in coordinating responses to allergens and pathogens therefore highlighting its importance in the body's defense mechanisms. As a member of the C-C motif chemokine family it shares structural characteristics with other chemokines like CCL11 which participate in similar immune cell trafficking functions.

Pathways

MCP4 is deeply involved in inflammatory and immune pathways specifically the chemokine signaling pathway and the cytokine-cytokine receptor interaction pathway. MCP4 guides immune cells allowing them to respond effectively to cytokine signals ensuring their migration to necessary sites. Through the chemokine signaling pathway it collaborates with related chemokines like CCL5 to enhance immune responses. Its interaction with these pathways highlights its role in both routine immunity and inflammatory responses positioning it as an important player in maintaining immune homeostasis.

MCP4 correlates with conditions such as asthma and allergic rhinitis. Both of these conditions involve chronic inflammation where MCP4's role becomes more pronounced. In asthma the protein contributes to the recruitment of eosinophils and other immune cells that drive the inflammatory processes. MCP4 interactions with proteins like CCR3 are significant in exacerbating symptoms of allergic disorders making it an important target for therapeutic research aimed at modulating inflammatory responses in these diseases.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens.
See full target information CCL13
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