Anti-MCP1抗体(ab9669)
Key features and details
- Rabbit polyclonal to MCP1
- Suitable for: WB, Sandwich ELISA
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-MCP1抗体
参阅全部 MCP1 一抗 -
描述
兔多克隆抗体to MCP1 -
宿主
Rabbit -
经测试应用
适用于: WB, Sandwich ELISAmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human MCP1.
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常规说明
Although some customers have been successful in IHC we no longer batch test in this application. For and IHC validated antibody please see ab73680
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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sELISA pair antibody
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) |
Use a concentration of 0.1 - 0.2 µg/ml.
To detect hMCAF/MCP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant MCP-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
| Sandwich ELISA |
Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [2.2-4H5-1A11] to MCP1 (ab9858).
To detect MCP-1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of ab9669 is required. This antigen affinity purified antibody, in conjunction with ab271250 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant MCP-1. |
| 说明 |
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WB
Use a concentration of 0.1 - 0.2 µg/ml. To detect hMCAF/MCP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant MCP-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
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Sandwich ELISA
Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [2.2-4H5-1A11] to MCP1 (ab9858). To detect MCP-1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of ab9669 is required. This antigen affinity purified antibody, in conjunction with ab271250 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant MCP-1. |
靶标
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功能
Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. -
序列相似性
Belongs to the intercrine beta (chemokine CC) family. -
翻译后修饰
Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 6347 Human
- Omim: 158105 Human
- SwissProt: P13500 Human
- Unigene: 303649 Human
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别名
- C-C motif chemokine 2 antibody
- CCL2 antibody
- CCL2_HUMAN antibody
see all
图片
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Western blot - Anti-MCP1 antibody (ab9669)
HL-60 cells were incubated at 37°C for 24hrs with vehicle control (0 µM) and varied concentrations of 2-Arachidonylglycerol (ab120098). Increased expression of MCP1 in HL-60 cells correlates with an increase in 2-Arachidonylglycerol concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab9669 at 1 µg/ml and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
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Sandwich ELISA - Anti-MCP1 antibody (ab9669) -
Western blot - Anti-MCP1 antibody (ab9669)All lanes : Anti-MCP1 antibody (ab9669) at 0.2 µg/ml
Lane 1 : Recombinant human MCP1 protein (ab73866) at 0.01 µg
Lane 2 : Recombinant human MCP1 protein (ab73866) at 0.001 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 1 minute -
Western blot - Anti-MCP1 antibody (ab9669)Image courtesy of Dr Laura Toma by Abreview.Anti-MCP1 antibody (ab9669) at 1/4000 dilution + lysate prepared from human smooth muscle treated with 100µg/ml glycated LDL at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/20000 dilution
Developed using the ECL technique.
Observed band size: 12-14 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
实验方案
数据表及文件
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Datasheet download
文献 (103)
ab9669 被引用在 103 文献中.
- Toma L et al. Oscillating Glucose Induces the Increase in Inflammatory Stress through Ninjurin-1 Up-Regulation and Stimulation of Transport Proteins in Human Endothelial Cells. Biomolecules 13:N/A (2023). PubMed: 37189375
- Lee AR et al. Establishment of a humanized mouse model of keloid diseases following the migration of patient immune cells to the lesion: Patient-derived keloid xenograft (PDKX) model. Exp Mol Med 55:1713-1719 (2023). PubMed: 37524866
- An R et al. SAMD1 attenuates antiphospholipid syndrome-induced pregnancy complications. Immun Inflamm Dis 11:e1006 (2023). PubMed: 37904675
- Peng H et al. MiR-15a-5p accelerated vascular smooth muscle cells viabilities and migratory abilities via targeting Bcl-2. Physiol Res 71:667-675 (2022). PubMed: 36047726
- Park TI et al. Routine culture and study of adult human brain cells from neurosurgical specimens. Nat Protoc 17:190-221 (2022). PubMed: 35022619
