AB9669

Anti-MCP1抗体

Name: MCP1抗体 (ab9669)| Abcam中文官网
Brand: Abcam Limited
SKU: AB9669
Price: 5234 CNY
Availability: InStock
Rating: 5 (4 reviews)

Anti-MCP1 antibody

(4 Reviews)

(119 Publications)

Rabbit Polyclonal MCP1 antibody. Suitable for WB, sELISA and reacts with Recombinant full length protein - Human samples. Cited in 119 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CCL2.

查看别名

MCP1, SCYA2, CCL2, C-C motif chemokine 2, HC11, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, Small-inducible cytokine A2, MCAF, MCP-1

4 Images

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Western blot - Anti-MCP1 antibody (AB9669)

HL-60 cells were incubated at 37°C for 24hrs with vehicle control (0 μM) and varied concentrations of 2-Arachidonylglycerol (ab120098). Increased expression of MCP1 in HL-60 cells correlates with an increase in 2-Arachidonylglycerol concentration, as described in literature.

Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab9669 at 1 μg/ml and ab8227 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

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Western blot - Anti-MCP1 antibody (ab9669)

Predicted band size: 11 kDa

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AbReview23240****

Western blot - Anti-MCP1 antibody (AB9669)

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Western blot - Anti-MCP1 antibody (ab9669) at 1/4000 dilution

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lysate prepared from human smooth muscle treated with 100µg/ml glycated LDL at 10 µg

Secondary

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Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab6721'>ab6721</a>) at 1/20000 dilution

Predicted band size: 11 kDa

Observed band size: 14-Dec kDa

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Exposure time: 1min

Image courtesy of Dr Laura Toma by Abreview.

Sandwich ELISA - Anti-MCP1 antibody (AB9669)

sELISA

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Sandwich ELISA - Anti-MCP1 antibody (AB9669)

Standard curve for MCP1 (Analyte : ab73866); dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal to MCP1 (ab9858) at 1μg/ml and Detector Antibody Rabbit polyclonal to MCP1 (ab9669) at 0.5μg/ml.

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Western blot - Anti-MCP1 antibody (AB9669)

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Western blot - Anti-MCP1 antibody (ab9669) at 0.2 µg/mL

Lane 1:

Western blot - Recombinant human MCP1 protein (<a href='/products/proteins-peptides/recombinant-human-mcp1-protein-ab73866'>ab73866</a>) at 0.01 µg

Lane 2:

Western blot - Recombinant human MCP1 protein (<a href='/products/proteins-peptides/recombinant-human-mcp1-protein-ab73866'>ab73866</a>) at 0.001 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 11 kDa

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Exposure time: 1min

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

轻链类型

unknown

不含载体蛋白

反应种属

Human

应用

sELISA, WB

applications

免疫原

Recombinant Fragment Protein within Human CCL2. The exact immunogen used to generate this antibody is proprietary information.

P13500

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "predicted", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.2 µg/mL", "WB-species-notes": "<p>To detect hMCAF/MCP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant MCP-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.</p>", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "0.5-2 µg/mL", "sELISA-species-notes": "<p>To detect MCP-1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of ab9669 is required. This antigen affinity purified antibody, in conjunction with <a href='/products/primary-antibodies/biotin-mcp1-antibody-ab271250'>ab271250</a> as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant MCP-1.</p>" } } }

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产品详情

Although some customers have been successful in IHC we no longer batch test in this application. For and IHC validated antibody please see ab73680

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性能和储存信息

形式

Lyophilized

复溶

Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.

纯化工艺

Affinity purification Immunogen

运输条件

Blue Ice

分装信息

Upon delivery aliquot

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MCP-1 also known as CCL2 is a chemokine involved in the recruitment of monocytes to sites of injury and inflammation. This protein consists of 76 amino acids and has a molecular weight of about 11 kDa. MCP-1 is expressed in a wide range of cell types including endothelial cells fibroblasts and monocytes. It acts by binding to the chemokine receptor CCR2 stimulating intracellular signaling pathways that mediate cell migration.

Biological function summary

The role of MCP-1 is significant in guiding immune cells to areas requiring immune response. It does not form part of a complex but operates as a singular entity to affect immune signaling. MCP-1 influences the behavior of monocytes and macrophages by inducing chemotaxis and activating their effector functions. Through this mechanism it aids in the inflammatory response and tissue repair.

Pathways

MCP-1 participates in the inflammatory pathways by cooperating with key proteins like CCR2. It facilitates the JAK-STAT signaling pathway which is vital for transmitting extracellular information into cellular responses. The interaction of MCP-1 with CCR2 influences the activation of downstream proteins like STAT3 enabling various immune responses. MCP-1 thereby plays a central role in modulating inflammation-related pathways.

MCP-1 has a connection to inflammatory bowel disease and atherosclerosis. In such conditions elevated levels of MCP-1 can exacerbate inflammatory responses by recruiting monocytes to dysfunctional sites worsening tissue damage. Through its interaction with CCR2 MCP-1 becomes a pivotal factor in chronic inflammation. Targeting this MCP-1/CCR2 axis can provide therapeutic opportunities for modulating disease progression in these inflammatory diseases.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Acts as a ligand for C-C chemokine receptor CCR2 (PubMed : 10529171, PubMed : 10587439, PubMed : 9837883). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (PubMed : 10587439, PubMed : 9837883). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (PubMed : 8195247, PubMed : 8627182, PubMed : 9792674). May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis (PubMed : 8107690).

See full target information CCL2

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文献 (119)

Recent publications for all applications. Explore the full list and refine your search

ACS omega 10:23196-23213 PubMed40521559

2025

Integrating Network Pharmacology and Experimental Validation to Elucidate the Mechanisms of Tang Shen Ping Decoction in Diabetic Kidney Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zhou,Yingjie Chen,Zirong Pan,Wanzhang Li,Meimei Su,Liangliang Zhang,Zijuan Zhou,Yongqiang Fang,Tianchi Hu,Yuanpeng Huang

Cells 14: PubMed39791741

2025

Mesenchymal Stem/Stromal Cells Reverse Adipose Tissue Inflammation in Pigs with Metabolic Syndrome and Renovascular Hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander B C Krueger,Xiangyang Zhu,Sarosh Siddiqi,Emma C Whitehead,Hui Tang,Kyra L Jordan,Amir Lerman,Lilach O Lerman

Journal of ovarian research 18:3 PubMed39773697

2025

Association between cytokines and progression-free survival in ovarian cancer following CRS/HIPEC treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Chao-Yu Chen,Ting-Yao Wang,Jing-Lan Liu,Yu-Che Ou,Li-Wen Lee,Chien-Hui Hung,Chuan-Pin Lee,Jrhau Lung

Scientific reports 14:13321 PubMed38858497

2024

Suppression of smooth muscle cell inflammation by myocardin-related transcription factors involves inactivation of TANK-binding kinase 1.

Applications

Unspecified application

Species

Unspecified reactive species

Elisabeth Bankell,Li Liu,Jennifer van der Horst,Catarina Rippe,Thomas A Jepps,Bengt-Olof Nilsson,Karl Swärd

International journal of molecular sciences 25: PubMed38791147

2024

The Elevated Inflammatory Status of Neutrophils Is Related to In-Hospital Complications in Patients with Acute Coronary Syndrome and Has Important Prognosis Value for Diabetic Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Barbu,Andreea Cristina Mihaila,Ana-Maria Gan,Letitia Ciortan,Razvan Daniel Macarie,Monica Madalina Tucureanu,Alexandru Filippi,Andra Ioana Stoenescu,Stefanita Victoria Petrea,Maya Simionescu,Serban Mihai Balanescu,Elena Butoi

Scientific reports 14:6772 PubMed38514705

2024

Characterization of diseased primary human hepatocytes in an all-human cell-based triculture system.

Applications

Unspecified application

Species

Unspecified reactive species

Justin J Odanga,Sharon M Anderson,Erick K Breathwaite,Sharon C Presnell,Edward L LeCluyse,Jingsong Chen,Jessica R Weaver

Immunity, inflammation and disease 11:e1006 PubMed37904675

2023

SAMD1 attenuates antiphospholipid syndrome-induced pregnancy complications.

Applications

Unspecified application

Species

Unspecified reactive species

Ran An,Yanqi Yang,Lei Liu,Peiling Li

Experimental & molecular medicine 55:1713-1719 PubMed37524866

2023

Establishment of a humanized mouse model of keloid diseases following the migration of patient immune cells to the lesion: Patient-derived keloid xenograft (PDKX) model.

Applications

Unspecified application

Species

Unspecified reactive species

A Ram Lee,Seon-Yeong Lee,Jeong Won Choi,In Gyu Um,Hyun Sik Na,Jung Ho Lee,Mi-La Cho

Journal of the American Heart Association 12:e024397 PubMed37026550

2023

Activation of the High Mobility Group Box 1/Receptor for Advanced Glycation Endproducts /NOD-like Receptor Family Pyrin Domain-Containing 3 Axis Under Chronic Intermittent Hypoxia Induction Promotes the Progression of Atherosclerosis in ApoE Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Haoqu Tan,Jinfang Hu,Wei Zuo,Yun Huang,Jian Cui,Fei Gong,Wei Bai

Biomolecules 13: PubMed37189375

2023

Oscillating Glucose Induces the Increase in Inflammatory Stress through Ninjurin-1 Up-Regulation and Stimulation of Transport Proteins in Human Endothelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Toma,Gabriela M Sanda,Camelia S Stancu,Loredan S Niculescu,Mina Raileanu,Anca V Sima

View all publications

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