Anti-MCP1抗体(ab7202)

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

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Thank you for your note.



This is to let you know that I have just contacted and asked our Account Department to raise a credit note for you - for the cost of one vial of ab7202. For your information, the internal reference note for this credit is CN20561. You can use this credit in the near future for any of the products which are in the catalogue.



I hope this helps and if I can assist further, please do not hesitate to contact me.

Thank you for your enquiry regarding ab7202 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is having problems with this antibody.

The protocol looks fine to me. It may well be the antibody has been accidentally damaged.

I can offer a new vial free of charge for your troubled customer but all our vials are from the same belled and purification. Could you please confirm the Abcam Order and the date of purchase and let me know how your customer would like to proceed with this enquiry.

I look forward to hearing from you soon.

Thank you for your phone call today, and I apologize for any miscommunication with my colleague yesterday. Your credit note ID for ab20641 is: ***. Your credit note ID for ab7202 is: ***. I am sorry that these antibodies did not perform as stated on the datasheet. If payment has already been made on the original orders and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.  

Thank you for your call today and for your questions about ab7202. As we discussed, we recommend using a microwave antigen retrieval method with this antibody. However, if the antibody is still staining the wrong localization, please let me know and we will be happy to send a replacement or issue a credit or refund. I look forward to hearing from you. Let me know if you have any questions.

I'm sorry to hear the customer is having problems with his new vial of ab7202. Thank you for taking the time to fill in the questionnaire, I can see clearly that the protocol looks very good and the antibody should perform if the customer has previously had good results. Could you please confirm the lot number or the order number for this vial as we have no record of a lot 7052 and confirm the storage conditions, my apologies for the inconvenience. Could you please also send us an image of the positive staining with lot 56165? Thank you for your patience, we look forward to hearing from you soon,

I'm sorry to hear you are having problem with ab7102. We have not tested it in mouse aorta or have any reviews yet from customers using it in aorta. So please do let us know when your staining works and we will reward you with an Amazon gift voucher (a review can be submitted via our online datasheet). I would like to suggest the following modifications to your protocol: -wash between all steps minimum 4 times 5min in PBS -fix for 10min in methanol or acetone pre-cooled in the freezer -block endogenous peroxidases with hydrogen peroxide (0.3% v/v in PBST) -block the slides with normal goat serum 10% v/v 1hr -use PBST (0.1-0.5%v/v triton) to dilute the serum, the primary and secondary antibody. -Incubate the biotin antibody for 2 hours at 1:400 in PBST, no BSA. -the dilution of the detection kit from Vector can be altered to decrease the background, make sure you prepare the ABC in PBS and leave to rest for 30min before applying onto slides. Try 1:500 for 1hr. It is best to leave it longer but more dilute. -When washing the slides after the DAB reaction, use Tris buffer 0.5M rather than PBS. This will decrease background as well. If you want to use the FITC-conjugated antibody dilute it in PBST (pH 7 or 8). For background problems please always check the secondary antibodies are not responsible. To do so, do the same protocol simply omitting the primary antibody. Please let me know if this helps and do not hesitate to contact us for further advice,

Thank you for your phone call. There are no special conditions that need to be followed to use ab7202 in IHC-frozen sections and so I suggest fixing your sections with acetone as per our general IHC protocol for frozen sections (the protocol can be found in the protocols tab of the online datasheet).