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AB9858

Anti-MCP1抗体[2.2-4H5-1A11]

Anti-MCP1 antibody [2.2-4H5-1A11]

0

(1 Review)

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(6 Publications)

Mouse Monoclonal MCP1 antibody. Suitable for ELISA, WB, sELISA, IHC-P and reacts with Recombinant full length protein - Human, Recombinant fragment - Human, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CCL2.

查看别名

MCP1, SCYA2, CCL2, C-C motif chemokine 2, HC11, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, Small-inducible cytokine A2, MCAF, MCP-1

2 Images
Sandwich ELISA - Anti-MCP1 antibody [2.2-4H5-1A11] (AB9858)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-MCP1 antibody [2.2-4H5-1A11] (AB9858)

In a sandwich ELISA (assuming 100 μl/well), a concentration of 1.0-2.0 μg/ml of this antibody will detect at least 100 pg/mL of Recombinant Human MCP-1 when used with Biotin Anti-MCP1 antibody (ab271250) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.

Sandwich ELISA - Anti-MCP1 antibody [2.2-4H5-1A11] (AB9858)
  • sELISA

Unknown

Sandwich ELISA - Anti-MCP1 antibody [2.2-4H5-1A11] (AB9858)

Standard curve for MCP1 (Analyte : ab73866); dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal to MCP1 (ab9858) at 1μg/ml and Detector Antibody Rabbit polyclonal to MCP1 (ab9669) at 0.5μg/ml.

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

2.2-4H5-1A11

亚型

IgG1

轻链类型

kappa

不含载体蛋白

No

反应种属

Human

应用

IHC-P, WB, ELISA, sELISA

applications

免疫原

Recombinant Full Length Protein corresponding to Human CCL2.

P13500

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反应性数据

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产品详情

This product is no longer batch tested in IHC, for an IHC validated antibody please see ab73680
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性能和储存信息

形式
Lyophilized
复溶
Reconstitute with 500µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
纯化工艺
Affinity purification Protein A
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MCP-1 also known as CCL2 is a chemokine involved in the recruitment of monocytes to sites of injury and inflammation. This protein consists of 76 amino acids and has a molecular weight of about 11 kDa. MCP-1 is expressed in a wide range of cell types including endothelial cells fibroblasts and monocytes. It acts by binding to the chemokine receptor CCR2 stimulating intracellular signaling pathways that mediate cell migration.
Biological function summary

The role of MCP-1 is significant in guiding immune cells to areas requiring immune response. It does not form part of a complex but operates as a singular entity to affect immune signaling. MCP-1 influences the behavior of monocytes and macrophages by inducing chemotaxis and activating their effector functions. Through this mechanism it aids in the inflammatory response and tissue repair.

Pathways

MCP-1 participates in the inflammatory pathways by cooperating with key proteins like CCR2. It facilitates the JAK-STAT signaling pathway which is vital for transmitting extracellular information into cellular responses. The interaction of MCP-1 with CCR2 influences the activation of downstream proteins like STAT3 enabling various immune responses. MCP-1 thereby plays a central role in modulating inflammation-related pathways.

MCP-1 has a connection to inflammatory bowel disease and atherosclerosis. In such conditions elevated levels of MCP-1 can exacerbate inflammatory responses by recruiting monocytes to dysfunctional sites worsening tissue damage. Through its interaction with CCR2 MCP-1 becomes a pivotal factor in chronic inflammation. Targeting this MCP-1/CCR2 axis can provide therapeutic opportunities for modulating disease progression in these inflammatory diseases.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Acts as a ligand for C-C chemokine receptor CCR2 (PubMed : 10529171, PubMed : 10587439, PubMed : 9837883). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (PubMed : 10587439, PubMed : 9837883). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (PubMed : 8195247, PubMed : 8627182, PubMed : 9792674). May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis (PubMed : 8107690).
See full target information CCL2
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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Cardiovascular diabetology 23:444 PubMed39696214

2024

Pericoronary adipose tissue radiomics features as imaging markers for coronary artery disease risk assessment: insights from gene expression analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Li,Weimin Zhang,Yahui Hu,Zheng Xu,Qiang Huo,Haicheng Qi,Qian Liu,Yan Xing

Heliyon 9:e17366 PubMed37426804

2023

circSnd1 promotes atherosclerosis progression through the miR-485-3p/Olr1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Yang,Yuhao Lin,Chao Wang,Pengcheng Fan

Diagnostics (Basel, Switzerland) 11: PubMed34067481

2021

Elevation of CD40/CD40L Inflammatory Pathway Molecules in Carotid Plaques from Moderate-and-Severe Obstructive Sleep Apnea Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Ewa Migacz,Wioletta Olejarz,Alicja Głuszko,Katarzyna Bednarek-Rajewska,Robert Proczka,David F Smith,Stacey L Ishman,Wojciech Kukwa

Oncology letters 15:7760-7768 PubMed29725470

2018

Mutant monocyte chemoattractant protein-1 protein (7ND) inhibits osteoclast differentiation and reduces oral squamous carcinoma cell bone invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Shuyu Luo,Chuanxiang Zhou,Jianming Zhang,Mengshan Chen,Hongjie Li,Shanchuan Zheng,Jingjing Quan

PloS one 9:e88952 PubMed24586454

2014

Monocyte chemotactic protein 1 (MCP-1) modulates pro-survival signaling to promote progression of head and neck squamous cell carcinoma.

Applications

IHC-P

Species

Human

Wen-Tsai Ji,Hau-Ren Chen,Chun-Hsuan Lin,Jeng-Woei Lee,Ching-Chih Lee

Frontiers in aging neuroscience 5:19 PubMed23675346

2013

Thrombin, a mediator of cerebrovascular inflammation in AD and hypoxia.

Applications

IHC-P

Species

Mouse

Debjani Tripathy,Alma Sanchez,Xiangling Yin,Jinhua Luo,Joseph Martinez,Paula Grammas
View all publications
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