重组Anti-MCM7/PRL抗体[EPR1973Y] (ab134194)
![Western blot - Anti-MCM7/PRL antibody [EPR1973Y] (ab134194)](https://www.abcam.cn/ps/products/134/ab134194/Images/ab134194-148526-anti-mcm7-antibody-epr1973y-western-blot.jpg "Western blot - Anti-MCM7/PRL antibody [EPR1973Y] (ab134194)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1973Y] to MCM7/PRL
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-MCM7/PRL抗体[EPR1973Y]
参阅全部 MCM7/PRL 一抗 -
描述
兔单克隆抗体[EPR1973Y] to MCM7/PRL -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: ICC/IF,IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human MCM7/PRL aa 1-100. The exact sequence is proprietary.
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阳性对照
- WB: HeLa and Jurkat cell lysates. Flow Cyt (intra): HeLa cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR1973Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134194于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/600.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/1000 - 1/2000. Predicted molecular weight: 81 kDa.
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| 说明 |
|---|
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Flow Cyt (Intra)
1/600. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/1000 - 1/2000. Predicted molecular weight: 81 kDa. |
靶标
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功能
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. -
序列相似性
Belongs to the MCM family.
Contains 1 MCM domain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 4176 Human
- Omim: 600592 Human
- SwissProt: P33993 Human
- Unigene: 438720 Human
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别名
- CDABP0042 antibody
- CDC 47 antibody
- CDC47 antibody
see all
图片
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Western blot - Anti-MCM7/PRL antibody [EPR1973Y] (ab134194)All lanes : Anti-MCM7/PRL antibody [EPR1973Y] (ab134194) at 1/2000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 81 kDa -
![Flow Cytometry (Intracellular) - Anti-MCM7/PRL antibody [EPR1973Y] (ab134194)](https://www.abcam.cn/ps/products/134/ab134194/Images/ab134194-4-anti-mcm7-prl-antibody-epr1973-flowcytometry-hela-human.jpg)
Flow Cytometry (Intracellular) - Anti-MCM7/PRL antibody [EPR1973Y] (ab134194)Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling MCM7/PRL with purified ab134194 at 1/600 dilution (1µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
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![Anti-MCM7/PRL antibody [EPR1973Y] (ab134194)](https://www.abcam.cn/ps/products/134/ab134194/Images/ab134194-2-benefits-of-recombinant-antibodies.png)
Anti-MCM7/PRL antibody [EPR1973Y] (ab134194)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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