Anti-MCM7/PRL抗体- BSA and Azide free (ab179904)
Key features and details
- Rabbit polyclonal to MCM7/PRL
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-MCM7/PRL抗体- BSA and Azide free
参阅全部 MCM7/PRL 一抗 -
描述
兔多克隆抗体to MCM7/PRL -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Cow -
免疫原
Recombinant fragment (His-tag) corresponding to Human MCM7/PRL aa 562-719 (C terminal).
Sequence:YIAMCREKQPMVPESLADYITAAYVEMRREAWASKDATYTSARTLLAILR LSTALARLRMVDVVEKEDVNEAIRLMEMSKDSLLGDKGQTARTQRPADVI FATVRELVS GGRSVRFSEAEQRCVSRGFTPAQFQAALDEYEELNVWQVNASRTRITFV
Database link: P33993 -
阳性对照
- Human HeLa, SiHa, C33A, WI38 cell extracts; Mouse NIH 3T3 cell extract.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
ab179904 is filter-sterilised. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab179904于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 81 kDa.
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ICC/IF |
1/200 - 1/1000.
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|
IP |
Use at an assay dependent concentration.
|
说明 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 81 kDa. |
ICC/IF
1/200 - 1/1000. |
IP
Use at an assay dependent concentration. |
靶标
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功能
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. -
序列相似性
Belongs to the MCM family.
Contains 1 MCM domain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 539924 Cow
- Entrez Gene: 4176 Human
- Entrez Gene: 17220 Mouse
- Omim: 600592 Human
- SwissProt: Q3ZBH9 Cow
- SwissProt: P33993 Human
- SwissProt: Q61881 Mouse
- Unigene: 438720 Human
see all -
别名
- CDABP0042 antibody
- CDC 47 antibody
- CDC47 antibody
see all
图片
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All lanes : Anti-MCM7/PRL antibody - BSA and Azide free (ab179904) at 1/1000 dilution
Lane 1 : SiHa whole cell extract
Lane 2 : C33A whole cell extract
Lane 3 : WI38 whole cell extract
Lysates/proteins at 100000 cells per lane.
Predicted band size: 81 kDa -
All lanes : Anti-MCM7/PRL antibody - BSA and Azide free (ab179904) at 1/2000 dilution
Lane 1 : HeLa whole cell extract
Lane 2 : HeLa whole cell extract treated with 100 nM adriamycin for 24 hours
Lane 3 : NIH 3T3 whole cell extract
Predicted band size: 81 kDa -
Immunoprecipitation analysis on crude extracts of fibroblast cell line WI38 using ab179904.
Lane 1; Immunoprecipitation with pre-immune serum.
Lane 2; Immnoprecipitation with ab179904 antiserum.
Cells were labeled with S35 methionine and MCM7/PRL was immunoprecipitated with ab179904 followed by SDS-PAGE and autoradiography.
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Immunofluorecent staining and confocal microscopic analysis of formaldehyde-fixed G1 phase HeLa cell nucleus, labeling MCM7/PRL using ab179904 at 1/200 dilution, after treatment with protein cross-linking reagent, DSP and chromatin extraction.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab179904 被引用在 4 文献中.
- Wang Y et al. LSD1 is required for euchromatic origin firing and replication timing. Signal Transduct Target Ther 7:102 (2022). PubMed: 35414135
- Fujita M et al. Nuclear organization of DNA replication initiation proteins in mammalian cells. J Biol Chem 277:10354-61 (2002). PubMed: 11779870
- Fujita M et al. In vivo interaction of human MCM heterohexameric complexes with chromatin. Possible involvement of ATP. J Biol Chem 272:10928-35 (1997). PubMed: 9099751
- Fujita M et al. hCDC47, a human member of the MCM family. Dissociation of the nucleus-bound form during S phase. J Biol Chem 271:4349-54 (1996). PubMed: 8626784